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[Determination of gallic acid in Melastoma dodecandrum by RP-HPLC].
Zhongguo Zhong Yao Za Zhi. 2005 Feb; 30(3):180-1.ZZ

Abstract

OBJECTIVE

To establish a RP-HPLC method for the determination of gallic acid in Chinese herb Melastoma dodecandrum, and to evaluate the quality of the herb.

METHOD

The RP-HPLC analysis was achieved by using a Polaris C18 column and tetrahydrofuran-methanol-0.2% phosphoric acid (0.5:0.5:99) as the mobile phase, with a flow rate of 1.0 mL x min(-1), and detected by UV at 274 nm.

RESULT

The content of gallic acid was from 0.020% to 0.081%, in ten groups of M. dodecandrum collected from different locations.

CONCLUSION

The method is a simple, convenient and rapid. It can be used for quality evaluation of M. dodecandrum.

Authors+Show Affiliations

School of Pharmacy, Guiyang Medical College, Guiyang 550004, China. hexun224@gmc.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
English Abstract
Journal Article

Language

chi

PubMed ID

15719632

Citation

He, Xun, et al. "[Determination of Gallic Acid in Melastoma Dodecandrum By RP-HPLC]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 30, no. 3, 2005, pp. 180-1.
He X, Li YJ, Liu LN, et al. [Determination of gallic acid in Melastoma dodecandrum by RP-HPLC]. Zhongguo Zhong Yao Za Zhi. 2005;30(3):180-1.
He, X., Li, Y. J., Liu, L. N., Lan, Y. Y., Wang, A. M., & Wang, Y. L. (2005). [Determination of gallic acid in Melastoma dodecandrum by RP-HPLC]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 30(3), 180-1.
He X, et al. [Determination of Gallic Acid in Melastoma Dodecandrum By RP-HPLC]. Zhongguo Zhong Yao Za Zhi. 2005;30(3):180-1. PubMed PMID: 15719632.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of gallic acid in Melastoma dodecandrum by RP-HPLC]. AU - He,Xun, AU - Li,Yong-jun, AU - Liu,Li-na, AU - Lan,Yan-yu, AU - Wang,Ai-min, AU - Wang,Yong-lin, PY - 2005/2/22/pubmed PY - 2005/7/13/medline PY - 2005/2/22/entrez SP - 180 EP - 1 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 30 IS - 3 N2 - OBJECTIVE: To establish a RP-HPLC method for the determination of gallic acid in Chinese herb Melastoma dodecandrum, and to evaluate the quality of the herb. METHOD: The RP-HPLC analysis was achieved by using a Polaris C18 column and tetrahydrofuran-methanol-0.2% phosphoric acid (0.5:0.5:99) as the mobile phase, with a flow rate of 1.0 mL x min(-1), and detected by UV at 274 nm. RESULT: The content of gallic acid was from 0.020% to 0.081%, in ten groups of M. dodecandrum collected from different locations. CONCLUSION: The method is a simple, convenient and rapid. It can be used for quality evaluation of M. dodecandrum. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/15719632/[Determination_of_gallic_acid_in_Melastoma_dodecandrum_by_RP_HPLC]_ DB - PRIME DP - Unbound Medicine ER -