Tags

Type your tag names separated by a space and hit enter

An alpha-glucan elicitor from the cell wall of a biocontrol binucleate Rhizoctonia isolate.
Carbohydr Res. 2005 Mar 21; 340(4):619-27.CR

Abstract

Binucleate Rhizoctonia (BNR) isolate (232-C6) is an effective biocontrol agent for protection of potato from Rhizoctonia canker, a disease caused by Rhizoctonia solani. Production of hydrolytic enzymes is one of the best known inducible defense responses following microbial infection. We isolated and characterized a cell wall alpha-glucan from BNR, which induces beta-1,3 glucanase activities in potato sprouts, the primary site of infection by R. solani. An autoclaving method, previously reported for isolation of oligosaccharide elicitors was used, and the glucan purified by chromatographic techniques. Maximal induction of beta-1,3 glucanase activity in potato sprouts was obtained with 250 microg of the alpha-glucan elicitor after 6 days from inoculation time. Both, BNR mycelium and the alpha-glucan produced a similar kinetic response of beta-1,3 glucanase. However, the alpha-glucan did not induce phytoalexin accumulation, previously correlated with the defense response. Uronic acids (approximately 10% with respect to total neutral sugars) were determined and identified as glucuronic acid by high-pH anion-exchange chromatography. Methylation analysis showed that the glucan consists of (1-->3) and (1-->4)-linked glucose units with preponderance of the first ones. Some of the (1-->4) linkages were branched at position 6. The glucan was partially degraded with amyloglucosidase. This, together with the NMR spectra data and the high optical rotation of the original (+195 degrees) and degraded glucans (+175 degrees) proved the alpha configuration. Further methylation of the amyloglucosidase degraded glucans indicated that they consist of (1-->3)-linked glucoses. The present study is the first report on the isolation and characterization of an alpha-glucan from Rhizoctonia, that may be important as a biocontrol factor.

Authors+Show Affiliations

Wolski EA
Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, PO Box 1245, Buenos Aires, Argentina.
Lima C
No affiliation info available
Agusti R
No affiliation info available
Daleo GR
No affiliation info available
Andreu AB
No affiliation info available
de Lederkremer RM
No affiliation info available

MeSH

Cell WallEnzyme InductionGlucan Endo-1,3-beta-D-GlucosidaseGlucansKineticsMethylationNuclear Magnetic Resonance, BiomolecularPest Control, BiologicalRhizoctonia

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15721332

Citation

Wolski, Erika A., et al. "An Alpha-glucan Elicitor From the Cell Wall of a Biocontrol Binucleate Rhizoctonia Isolate." Carbohydrate Research, vol. 340, no. 4, 2005, pp. 619-27.
Wolski EA, Lima C, Agusti R, et al. An alpha-glucan elicitor from the cell wall of a biocontrol binucleate Rhizoctonia isolate. Carbohydr Res. 2005;340(4):619-27.
Wolski, E. A., Lima, C., Agusti, R., Daleo, G. R., Andreu, A. B., & de Lederkremer, R. M. (2005). An alpha-glucan elicitor from the cell wall of a biocontrol binucleate Rhizoctonia isolate. Carbohydrate Research, 340(4), 619-27.
Wolski EA, et al. An Alpha-glucan Elicitor From the Cell Wall of a Biocontrol Binucleate Rhizoctonia Isolate. Carbohydr Res. 2005 Mar 21;340(4):619-27. PubMed PMID: 15721332.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An alpha-glucan elicitor from the cell wall of a biocontrol binucleate Rhizoctonia isolate. AU - Wolski,Erika A, AU - Lima,Carlos, AU - Agusti,Rosalía, AU - Daleo,Gustavo R, AU - Andreu,Adriana B, AU - de Lederkremer,Rosa M, PY - 2004/06/29/received PY - 2004/12/10/accepted PY - 2005/2/22/pubmed PY - 2005/7/20/medline PY - 2005/2/22/entrez SP - 619 EP - 27 JF - Carbohydrate research JO - Carbohydr Res VL - 340 IS - 4 N2 - Binucleate Rhizoctonia (BNR) isolate (232-C6) is an effective biocontrol agent for protection of potato from Rhizoctonia canker, a disease caused by Rhizoctonia solani. Production of hydrolytic enzymes is one of the best known inducible defense responses following microbial infection. We isolated and characterized a cell wall alpha-glucan from BNR, which induces beta-1,3 glucanase activities in potato sprouts, the primary site of infection by R. solani. An autoclaving method, previously reported for isolation of oligosaccharide elicitors was used, and the glucan purified by chromatographic techniques. Maximal induction of beta-1,3 glucanase activity in potato sprouts was obtained with 250 microg of the alpha-glucan elicitor after 6 days from inoculation time. Both, BNR mycelium and the alpha-glucan produced a similar kinetic response of beta-1,3 glucanase. However, the alpha-glucan did not induce phytoalexin accumulation, previously correlated with the defense response. Uronic acids (approximately 10% with respect to total neutral sugars) were determined and identified as glucuronic acid by high-pH anion-exchange chromatography. Methylation analysis showed that the glucan consists of (1-->3) and (1-->4)-linked glucose units with preponderance of the first ones. Some of the (1-->4) linkages were branched at position 6. The glucan was partially degraded with amyloglucosidase. This, together with the NMR spectra data and the high optical rotation of the original (+195 degrees) and degraded glucans (+175 degrees) proved the alpha configuration. Further methylation of the amyloglucosidase degraded glucans indicated that they consist of (1-->3)-linked glucoses. The present study is the first report on the isolation and characterization of an alpha-glucan from Rhizoctonia, that may be important as a biocontrol factor. SN - 0008-6215 UR - https://www.unboundmedicine.com/medline/citation/15721332/An_alpha_glucan_elicitor_from_the_cell_wall_of_a_biocontrol_binucleate_Rhizoctonia_isolate_ DB - PRIME DP - Unbound Medicine ER -