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Effects of angiotensin II type 1 receptor blockade on the oxidative stress in spontaneously hypertensive rat tissues.
Regul Pept. 2005 May 15; 128(1):1-5.RP

Abstract

The aim of this work was to investigate the production of oxidative damage in homogenized kidney, liver and brain of spontaneously hypertensive rats (SHR), as well as the involvement of angiotensin (Ang) II in this process. Groups of 12-week-old SHR and Wistar Kyoto rats (WKY) were given 10 mg/kg/day losartan in the drinking water during 14 days. Other groups of WKY and SHR without treatment were used as controls. The production of thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH) and the activity of the antioxidant enzymes catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (Gpx) were determined. No significant difference in TBARS was observed between untreated SHR or WKY rats; GSH content was lower in the liver but higher in the brain of SHR compared to WKY rats. In tissues from the SHR group, SOD and Gpx activities were reduced, whereas CAT activity was slightly increased in kidney. TBARS levels did not change in WKY rats after losartan administration, but were reduced in SHR liver and brain. Losartan treatment decreased GSH content in WKY kidney, but increased GSH in SHR liver. The activity of the antioxidant enzymes was not modified by losartan in WKY rats; however, their activities increased in tissues from treated SHR. The lower activity of antioxidant enzymes in tissues from hypertensive rats compared to those detected in normotensive controls, indicates oxidative stress production. Ang II seems to play no role in this process in normotensive animals, although AT1 receptor blockade in SHR enhances the enzymatic activity indicating that Ang II is implicated in oxidative stress generation in the hypertensive animals.

Authors+Show Affiliations

Departamento de Química Biológica, Instituto de Química y Fisicoquímica Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113 Buenos Aires, Argentina.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15721481

Citation

Polizio, Ariel Héctor, and Clara Peña. "Effects of Angiotensin II Type 1 Receptor Blockade On the Oxidative Stress in Spontaneously Hypertensive Rat Tissues." Regulatory Peptides, vol. 128, no. 1, 2005, pp. 1-5.
Polizio AH, Peña C. Effects of angiotensin II type 1 receptor blockade on the oxidative stress in spontaneously hypertensive rat tissues. Regul Pept. 2005;128(1):1-5.
Polizio, A. H., & Peña, C. (2005). Effects of angiotensin II type 1 receptor blockade on the oxidative stress in spontaneously hypertensive rat tissues. Regulatory Peptides, 128(1), 1-5.
Polizio AH, Peña C. Effects of Angiotensin II Type 1 Receptor Blockade On the Oxidative Stress in Spontaneously Hypertensive Rat Tissues. Regul Pept. 2005 May 15;128(1):1-5. PubMed PMID: 15721481.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of angiotensin II type 1 receptor blockade on the oxidative stress in spontaneously hypertensive rat tissues. AU - Polizio,Ariel Héctor, AU - Peña,Clara, PY - 2004/09/13/received PY - 2004/12/01/accepted PY - 2005/2/22/pubmed PY - 2005/9/27/medline PY - 2005/2/22/entrez SP - 1 EP - 5 JF - Regulatory peptides JO - Regul Pept VL - 128 IS - 1 N2 - The aim of this work was to investigate the production of oxidative damage in homogenized kidney, liver and brain of spontaneously hypertensive rats (SHR), as well as the involvement of angiotensin (Ang) II in this process. Groups of 12-week-old SHR and Wistar Kyoto rats (WKY) were given 10 mg/kg/day losartan in the drinking water during 14 days. Other groups of WKY and SHR without treatment were used as controls. The production of thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH) and the activity of the antioxidant enzymes catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (Gpx) were determined. No significant difference in TBARS was observed between untreated SHR or WKY rats; GSH content was lower in the liver but higher in the brain of SHR compared to WKY rats. In tissues from the SHR group, SOD and Gpx activities were reduced, whereas CAT activity was slightly increased in kidney. TBARS levels did not change in WKY rats after losartan administration, but were reduced in SHR liver and brain. Losartan treatment decreased GSH content in WKY kidney, but increased GSH in SHR liver. The activity of the antioxidant enzymes was not modified by losartan in WKY rats; however, their activities increased in tissues from treated SHR. The lower activity of antioxidant enzymes in tissues from hypertensive rats compared to those detected in normotensive controls, indicates oxidative stress production. Ang II seems to play no role in this process in normotensive animals, although AT1 receptor blockade in SHR enhances the enzymatic activity indicating that Ang II is implicated in oxidative stress generation in the hypertensive animals. SN - 0167-0115 UR - https://www.unboundmedicine.com/medline/citation/15721481/Effects_of_angiotensin_II_type_1_receptor_blockade_on_the_oxidative_stress_in_spontaneously_hypertensive_rat_tissues_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0167-0115(04)00447-1 DB - PRIME DP - Unbound Medicine ER -