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Changes in lipid metabolism and antioxidant defense status in spontaneously hypertensive rats and Wistar rats fed a diet enriched with fructose and saturated fatty acids.
Nutrition. 2005 Feb; 21(2):240-8.N

Abstract

OBJECTIVE

Larger doses of fructose and saturated fat have been associated with oxidative stress and development of hypertension. The effects of modest amounts of fructose and saturated fatty acids on oxidative stress are unknown.

METHODS

To increase knowledge on this question, 10-wk-old spontaneously hypertensive rats and Wistar rats were fed for 8 wk with a control diet or an experimental diet enriched with fructose (18%) and saturated fatty acids (11%; FS diet). The total antioxidant status of organs and red blood cells was assayed by monitoring the rate of free radical-induced red blood cell hemolysis. Sensitivity of very low-density lipoprotein and low-density lipoprotein (VLDL-LDL) to copper-induced lipid peroxidation was determined as the production of thiobarbituric acid-reactive substances. Antioxidant enzymes and vitamins were also measured to establish the oxidative stress effect.

RESULTS

The FS diet did not affect blood pressure in either strain, but it increased plasma insulin concentrations only in Wistar rats without affecting those of glucose of either strain. The FS diet significantly enhanced plasma and VLDL-LDL triacylglycerol concentrations without affecting concentrations of VLDL-LDL thiobarbituric acid-reactive substances. The decreased content of arachidonic acid and total polyunsaturated fatty acids in VLDL-LDL by the FS diet may have prevented lipid peroxidation in this fraction. Moreover, FS consumption by both strains was accompanied by a significant increase in total antioxidant capacity of adipose tissue, muscle, heart, and liver. This may have resulted from increased tissue ascorbic acid levels and glutathione peroxidase and glutathione reductase activities in tissues.

CONCLUSIONS

These findings clearly indicate that the FS diet did not alter blood pressure of spontaneously hypertensive rats and Wistar rats. The FS diet resulted in hypertriglyceridemia but increased the total antioxidant status, which may prevent lipid peroxidation in these rats.

Authors+Show Affiliations

Université de Bourgogne, UPRES Lipides Nutrition EA 2422, Faculté des Sciences Gabriel, Dijon, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15723754

Citation

Girard, Aurélie, et al. "Changes in Lipid Metabolism and Antioxidant Defense Status in Spontaneously Hypertensive Rats and Wistar Rats Fed a Diet Enriched With Fructose and Saturated Fatty Acids." Nutrition (Burbank, Los Angeles County, Calif.), vol. 21, no. 2, 2005, pp. 240-8.
Girard A, Madani S, El Boustani ES, et al. Changes in lipid metabolism and antioxidant defense status in spontaneously hypertensive rats and Wistar rats fed a diet enriched with fructose and saturated fatty acids. Nutrition. 2005;21(2):240-8.
Girard, A., Madani, S., El Boustani, E. S., Belleville, J., & Prost, J. (2005). Changes in lipid metabolism and antioxidant defense status in spontaneously hypertensive rats and Wistar rats fed a diet enriched with fructose and saturated fatty acids. Nutrition (Burbank, Los Angeles County, Calif.), 21(2), 240-8.
Girard A, et al. Changes in Lipid Metabolism and Antioxidant Defense Status in Spontaneously Hypertensive Rats and Wistar Rats Fed a Diet Enriched With Fructose and Saturated Fatty Acids. Nutrition. 2005;21(2):240-8. PubMed PMID: 15723754.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in lipid metabolism and antioxidant defense status in spontaneously hypertensive rats and Wistar rats fed a diet enriched with fructose and saturated fatty acids. AU - Girard,Aurélie, AU - Madani,Sihem, AU - El Boustani,Es Saddik, AU - Belleville,Jacques, AU - Prost,Josiane, PY - 2003/03/13/received PY - 2003/03/13/revised PY - 2004/04/20/accepted PY - 2005/2/23/pubmed PY - 2005/5/12/medline PY - 2005/2/23/entrez SP - 240 EP - 8 JF - Nutrition (Burbank, Los Angeles County, Calif.) JO - Nutrition VL - 21 IS - 2 N2 - OBJECTIVE: Larger doses of fructose and saturated fat have been associated with oxidative stress and development of hypertension. The effects of modest amounts of fructose and saturated fatty acids on oxidative stress are unknown. METHODS: To increase knowledge on this question, 10-wk-old spontaneously hypertensive rats and Wistar rats were fed for 8 wk with a control diet or an experimental diet enriched with fructose (18%) and saturated fatty acids (11%; FS diet). The total antioxidant status of organs and red blood cells was assayed by monitoring the rate of free radical-induced red blood cell hemolysis. Sensitivity of very low-density lipoprotein and low-density lipoprotein (VLDL-LDL) to copper-induced lipid peroxidation was determined as the production of thiobarbituric acid-reactive substances. Antioxidant enzymes and vitamins were also measured to establish the oxidative stress effect. RESULTS: The FS diet did not affect blood pressure in either strain, but it increased plasma insulin concentrations only in Wistar rats without affecting those of glucose of either strain. The FS diet significantly enhanced plasma and VLDL-LDL triacylglycerol concentrations without affecting concentrations of VLDL-LDL thiobarbituric acid-reactive substances. The decreased content of arachidonic acid and total polyunsaturated fatty acids in VLDL-LDL by the FS diet may have prevented lipid peroxidation in this fraction. Moreover, FS consumption by both strains was accompanied by a significant increase in total antioxidant capacity of adipose tissue, muscle, heart, and liver. This may have resulted from increased tissue ascorbic acid levels and glutathione peroxidase and glutathione reductase activities in tissues. CONCLUSIONS: These findings clearly indicate that the FS diet did not alter blood pressure of spontaneously hypertensive rats and Wistar rats. The FS diet resulted in hypertriglyceridemia but increased the total antioxidant status, which may prevent lipid peroxidation in these rats. SN - 0899-9007 UR - https://www.unboundmedicine.com/medline/citation/15723754/Changes_in_lipid_metabolism_and_antioxidant_defense_status_in_spontaneously_hypertensive_rats_and_Wistar_rats_fed_a_diet_enriched_with_fructose_and_saturated_fatty_acids_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0899-9007(04)00245-X DB - PRIME DP - Unbound Medicine ER -