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Molecular characterization of DNA sequences from the Primula vulgaris S-locus.
J Exp Bot. 2005 Apr; 56(414):1177-88.JE

Abstract

Primula species provide possibly the best known examples of heteromorphic flower development and this breeding system has attracted considerable attention, including that of Charles Darwin. However, despite considerable recent advances in molecular genetics, nothing is known about the molecular basis of floral heteromorphy. The first molecular marker for the Primula S-locus is reported here. This DNA sequence was identified by random amplification of polymorphic DNA (RAPD)-PCR, further defined as a sequence characterized amplified region (SCAR) marker, and subsequently shown to correspond to a restriction fragment length polymorphism (RFLP) that is linked to the thrum allele of the Primula S-locus. The sequence of 8.8 kb of genomic DNA encompassing this thrum-specific RFLP is presented. Analysis of this DNA reveals a highly repetitive sequence structure similar to that found at the S-locus in other species; it also contains sequences similar to elements of a Gypsy-like retrotransposon. The identification of a specific DNA sequence associated with the thrum allele of the Primula S-locus provides the first molecular probe with which to investigate the molecular basis of heteromorphic flower development in Primula.

Authors+Show Affiliations

Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15723823

Citation

Manfield, Iain W., et al. "Molecular Characterization of DNA Sequences From the Primula Vulgaris S-locus." Journal of Experimental Botany, vol. 56, no. 414, 2005, pp. 1177-88.
Manfield IW, Pavlov VK, Li J, et al. Molecular characterization of DNA sequences from the Primula vulgaris S-locus. J Exp Bot. 2005;56(414):1177-88.
Manfield, I. W., Pavlov, V. K., Li, J., Cook, H. E., Hummel, F., & Gilmartin, P. M. (2005). Molecular characterization of DNA sequences from the Primula vulgaris S-locus. Journal of Experimental Botany, 56(414), 1177-88.
Manfield IW, et al. Molecular Characterization of DNA Sequences From the Primula Vulgaris S-locus. J Exp Bot. 2005;56(414):1177-88. PubMed PMID: 15723823.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of DNA sequences from the Primula vulgaris S-locus. AU - Manfield,Iain W, AU - Pavlov,Vassily K, AU - Li,Jinhong, AU - Cook,Holly E, AU - Hummel,Florian, AU - Gilmartin,Philip M, Y1 - 2005/02/21/ PY - 2005/2/23/pubmed PY - 2005/7/6/medline PY - 2005/2/23/entrez SP - 1177 EP - 88 JF - Journal of experimental botany JO - J Exp Bot VL - 56 IS - 414 N2 - Primula species provide possibly the best known examples of heteromorphic flower development and this breeding system has attracted considerable attention, including that of Charles Darwin. However, despite considerable recent advances in molecular genetics, nothing is known about the molecular basis of floral heteromorphy. The first molecular marker for the Primula S-locus is reported here. This DNA sequence was identified by random amplification of polymorphic DNA (RAPD)-PCR, further defined as a sequence characterized amplified region (SCAR) marker, and subsequently shown to correspond to a restriction fragment length polymorphism (RFLP) that is linked to the thrum allele of the Primula S-locus. The sequence of 8.8 kb of genomic DNA encompassing this thrum-specific RFLP is presented. Analysis of this DNA reveals a highly repetitive sequence structure similar to that found at the S-locus in other species; it also contains sequences similar to elements of a Gypsy-like retrotransposon. The identification of a specific DNA sequence associated with the thrum allele of the Primula S-locus provides the first molecular probe with which to investigate the molecular basis of heteromorphic flower development in Primula. SN - 0022-0957 UR - https://www.unboundmedicine.com/medline/citation/15723823/Molecular_characterization_of_DNA_sequences_from_the_Primula_vulgaris_S_locus_ L2 - https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/eri110 DB - PRIME DP - Unbound Medicine ER -