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CD25(+)CD4(+) regulatory T cells exert in vitro suppressive activity independent of CTLA-4.
Int Immunol. 2005 Apr; 17(4):421-7.II

Abstract

Cytotoxic T lymphocyte antigen-4 (CTLA-4) is constitutively expressed on CD25(+)CD4(+) regulatory T cells (Treg) and is suggested to play a role in Treg-mediated suppression. However, the results of analysis with anti-CTLA-4 have been controversial. We addressed this issue by analyzing mice over-expressing or deficient in CTLA-4. For over-expression, CTLA-4 transgenic mice expressing a full-length (FL) or a truncated (TL) mutant of CTLA-4 were analyzed. FL T cells expressed similar levels of CTLA-4 to Treg, whereas TL T cells expressed much higher levels on the cell surface. The number of Treg in both mice was decreased, although Foxp3 expression was not altered. Treg from both mice exerted suppressive activity, whereas CD25(-) T cells from FL mice showed no suppression. Furthermore, CD25(+)CD4 thymocytes from young CTLA-4-deficient mice were analyzed and found to exhibit suppressive activity. These results indicate that Treg exert in vitro suppressive activity independent of CTLA-4 expression.

Authors+Show Affiliations

Department of Molecular Genetics, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15724061

Citation

Kataoka, Hiroshi, et al. "CD25(+)CD4(+) Regulatory T Cells Exert in Vitro Suppressive Activity Independent of CTLA-4." International Immunology, vol. 17, no. 4, 2005, pp. 421-7.
Kataoka H, Takahashi S, Takase K, et al. CD25(+)CD4(+) regulatory T cells exert in vitro suppressive activity independent of CTLA-4. Int Immunol. 2005;17(4):421-7.
Kataoka, H., Takahashi, S., Takase, K., Yamasaki, S., Yokosuka, T., Koike, T., & Saito, T. (2005). CD25(+)CD4(+) regulatory T cells exert in vitro suppressive activity independent of CTLA-4. International Immunology, 17(4), 421-7.
Kataoka H, et al. CD25(+)CD4(+) Regulatory T Cells Exert in Vitro Suppressive Activity Independent of CTLA-4. Int Immunol. 2005;17(4):421-7. PubMed PMID: 15724061.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CD25(+)CD4(+) regulatory T cells exert in vitro suppressive activity independent of CTLA-4. AU - Kataoka,Hiroshi, AU - Takahashi,Shigekazu, AU - Takase,Kan, AU - Yamasaki,Sho, AU - Yokosuka,Tadashi, AU - Koike,Takao, AU - Saito,Takashi, Y1 - 2005/02/21/ PY - 2005/2/23/pubmed PY - 2005/8/10/medline PY - 2005/2/23/entrez SP - 421 EP - 7 JF - International immunology JO - Int Immunol VL - 17 IS - 4 N2 - Cytotoxic T lymphocyte antigen-4 (CTLA-4) is constitutively expressed on CD25(+)CD4(+) regulatory T cells (Treg) and is suggested to play a role in Treg-mediated suppression. However, the results of analysis with anti-CTLA-4 have been controversial. We addressed this issue by analyzing mice over-expressing or deficient in CTLA-4. For over-expression, CTLA-4 transgenic mice expressing a full-length (FL) or a truncated (TL) mutant of CTLA-4 were analyzed. FL T cells expressed similar levels of CTLA-4 to Treg, whereas TL T cells expressed much higher levels on the cell surface. The number of Treg in both mice was decreased, although Foxp3 expression was not altered. Treg from both mice exerted suppressive activity, whereas CD25(-) T cells from FL mice showed no suppression. Furthermore, CD25(+)CD4 thymocytes from young CTLA-4-deficient mice were analyzed and found to exhibit suppressive activity. These results indicate that Treg exert in vitro suppressive activity independent of CTLA-4 expression. SN - 0953-8178 UR - https://www.unboundmedicine.com/medline/citation/15724061/CD25_+_CD4_+__regulatory_T_cells_exert_in_vitro_suppressive_activity_independent_of_CTLA_4_ L2 - https://academic.oup.com/intimm/article-lookup/doi/10.1093/intimm/dxh221 DB - PRIME DP - Unbound Medicine ER -