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[Multiplex PCR assay against E. coli O157 in foodstuffs].
Wei Sheng Yan Jiu. 2004 Nov; 33(6):716-9.WS

Abstract

A multiplex PCR assay for detection of Escherichia coli O157:H7 was developed with 4 pairs primers which focus on its specific fliC, rfbE, SLT I and SLT II genes. The target genes fragments of the PCR assay were 560 bp, 678 bp, 210 bp and 484 bp. The multiplex PCR had very high specificity to match all the 71 tested strains including 58 E. coli strains and other 18 strains. The strain of E. coli O157:H7 was identified in one step, and the type of SLT was also identified as well. With the enrichment procedure, the strain of E. coli O157:H7 were recovered at the detection limit of 0.1 cfu/ml from artificial inoculated pasteurized milk.

Authors+Show Affiliations

Zhao Z
Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Liu X
No affiliation info available

MeSH

AnimalsDNA PrimersEscherichia coli O157Food MicrobiologyMilkPolymerase Chain ReactionSensitivity and Specificity

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15727186

Citation

Zhao, Zhijing, and Xiumei Liu. "[Multiplex PCR Assay Against E. Coli O157 in Foodstuffs]." Wei Sheng Yan Jiu = Journal of Hygiene Research, vol. 33, no. 6, 2004, pp. 716-9.
Zhao Z, Liu X. [Multiplex PCR assay against E. coli O157 in foodstuffs]. Wei Sheng Yan Jiu. 2004;33(6):716-9.
Zhao, Z., & Liu, X. (2004). [Multiplex PCR assay against E. coli O157 in foodstuffs]. Wei Sheng Yan Jiu = Journal of Hygiene Research, 33(6), 716-9.
Zhao Z, Liu X. [Multiplex PCR Assay Against E. Coli O157 in Foodstuffs]. Wei Sheng Yan Jiu. 2004;33(6):716-9. PubMed PMID: 15727186.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Multiplex PCR assay against E. coli O157 in foodstuffs]. AU - Zhao,Zhijing, AU - Liu,Xiumei, PY - 2005/2/25/pubmed PY - 2006/9/9/medline PY - 2005/2/25/entrez SP - 716 EP - 9 JF - Wei sheng yan jiu = Journal of hygiene research JO - Wei Sheng Yan Jiu VL - 33 IS - 6 N2 - A multiplex PCR assay for detection of Escherichia coli O157:H7 was developed with 4 pairs primers which focus on its specific fliC, rfbE, SLT I and SLT II genes. The target genes fragments of the PCR assay were 560 bp, 678 bp, 210 bp and 484 bp. The multiplex PCR had very high specificity to match all the 71 tested strains including 58 E. coli strains and other 18 strains. The strain of E. coli O157:H7 was identified in one step, and the type of SLT was also identified as well. With the enrichment procedure, the strain of E. coli O157:H7 were recovered at the detection limit of 0.1 cfu/ml from artificial inoculated pasteurized milk. SN - 1000-8020 UR - https://www.unboundmedicine.com/medline/citation/15727186/[Multiplex_PCR_assay_against_E__coli_O157_in_foodstuffs]_ DB - PRIME DP - Unbound Medicine ER -