Tags

Type your tag names separated by a space and hit enter

Protection of human conjunctival fibroblasts from NO-induced apoptosis by interleukin-4 or interleukin-13.
Invest Ophthalmol Vis Sci. 2005 Mar; 46(3):797-802.IO

Abstract

PURPOSE

To examine the possible roles of T helper type 2 (Th2) cell-derived cytokines in formation of the giant papillae characteristic in individuals with vernal keratoconjunctivitis, the effects of these cytokines on the proliferation and apoptosis of cultured human conjunctival fibroblasts were investigated.

METHODS

Apoptosis was induced by the NO donor sodium nitroprusside. Cell viability was determined by measurement of mitochondrial metabolic activity, and apoptotic cells were identified on the basis either of nuclear morphology after staining with 4',6-diamidino-2-phenylindole or of TUNEL staining. The activation of antiapoptotic signaling mediated by the protein kinase Akt was assessed by immunoblot analysis and by an in vitro kinase assay. Expression of interleukin (IL)-4 and IL-13 receptor subunits was examined by reverse transcription and polymerase chain reaction analysis and by flow cytometry.

RESULTS

IL-4 and IL-13, but not IL-5, IL-9, or IL-10, induced the proliferation of conjunctival fibroblasts as well as protecting these cells from NO-induced apoptosis. Both IL-4 and IL-13 induced the phosphorylation of Akt and increased the kinase activity of this enzyme in a manner that was sensitive to the phosphatidylinositol 3-kinase inhibitors LY294002 or wortmannin. These inhibitors also blocked the antiapoptotic effects of IL-4 and IL-13. Transcripts encoding IL-4 and IL-13 receptor components were detected in conjunctival fibroblasts, and the proteins were expressed at the cell surface.

CONCLUSIONS

Among the various Th2 cytokines tested, only IL-4 and IL-13 induced the proliferation of human conjunctival fibroblasts and protected these cells from apoptosis. These effects may contribute to the formation of giant papillae in individuals with vernal keratoconjunctivitis.

Authors+Show Affiliations

Department of Biomolecular Recognition, Yamaguchi University School of Medicine, Ube City, Yamaguchi 755-8505, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15728533

Citation

Fujitsu, Youichiro, et al. "Protection of Human Conjunctival Fibroblasts From NO-induced Apoptosis By Interleukin-4 or Interleukin-13." Investigative Ophthalmology & Visual Science, vol. 46, no. 3, 2005, pp. 797-802.
Fujitsu Y, Fukuda K, Kimura K, et al. Protection of human conjunctival fibroblasts from NO-induced apoptosis by interleukin-4 or interleukin-13. Invest Ophthalmol Vis Sci. 2005;46(3):797-802.
Fujitsu, Y., Fukuda, K., Kimura, K., Seki, K., Kumagai, N., & Nishida, T. (2005). Protection of human conjunctival fibroblasts from NO-induced apoptosis by interleukin-4 or interleukin-13. Investigative Ophthalmology & Visual Science, 46(3), 797-802.
Fujitsu Y, et al. Protection of Human Conjunctival Fibroblasts From NO-induced Apoptosis By Interleukin-4 or Interleukin-13. Invest Ophthalmol Vis Sci. 2005;46(3):797-802. PubMed PMID: 15728533.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protection of human conjunctival fibroblasts from NO-induced apoptosis by interleukin-4 or interleukin-13. AU - Fujitsu,Youichiro, AU - Fukuda,Ken, AU - Kimura,Kazuhiro, AU - Seki,Keisuke, AU - Kumagai,Naoki, AU - Nishida,Teruo, PY - 2005/2/25/pubmed PY - 2005/4/14/medline PY - 2005/2/25/entrez SP - 797 EP - 802 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 46 IS - 3 N2 - PURPOSE: To examine the possible roles of T helper type 2 (Th2) cell-derived cytokines in formation of the giant papillae characteristic in individuals with vernal keratoconjunctivitis, the effects of these cytokines on the proliferation and apoptosis of cultured human conjunctival fibroblasts were investigated. METHODS: Apoptosis was induced by the NO donor sodium nitroprusside. Cell viability was determined by measurement of mitochondrial metabolic activity, and apoptotic cells were identified on the basis either of nuclear morphology after staining with 4',6-diamidino-2-phenylindole or of TUNEL staining. The activation of antiapoptotic signaling mediated by the protein kinase Akt was assessed by immunoblot analysis and by an in vitro kinase assay. Expression of interleukin (IL)-4 and IL-13 receptor subunits was examined by reverse transcription and polymerase chain reaction analysis and by flow cytometry. RESULTS: IL-4 and IL-13, but not IL-5, IL-9, or IL-10, induced the proliferation of conjunctival fibroblasts as well as protecting these cells from NO-induced apoptosis. Both IL-4 and IL-13 induced the phosphorylation of Akt and increased the kinase activity of this enzyme in a manner that was sensitive to the phosphatidylinositol 3-kinase inhibitors LY294002 or wortmannin. These inhibitors also blocked the antiapoptotic effects of IL-4 and IL-13. Transcripts encoding IL-4 and IL-13 receptor components were detected in conjunctival fibroblasts, and the proteins were expressed at the cell surface. CONCLUSIONS: Among the various Th2 cytokines tested, only IL-4 and IL-13 induced the proliferation of human conjunctival fibroblasts and protected these cells from apoptosis. These effects may contribute to the formation of giant papillae in individuals with vernal keratoconjunctivitis. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/15728533/Protection_of_human_conjunctival_fibroblasts_from_NO_induced_apoptosis_by_interleukin_4_or_interleukin_13_ L2 - https://iovs.arvojournals.org/article.aspx?doi=10.1167/iovs.04-1016 DB - PRIME DP - Unbound Medicine ER -