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MyoD-cre transgenic mice: a model for conditional mutagenesis and lineage tracing of skeletal muscle.
Genesis. 2005 Mar; 41(3):116-21.G

Abstract

The Cre-loxP recombination system has been used to great advantage in vivo for conditional gene targeting, lineage tracing, and other applications. To express cre in skeletal myoblasts and muscle fibers, we utilized the well-characterized transcriptional regulatory regions of the muscle determination gene, MyoD. Transgenic mouse lines were produced (F3/-2.5cre) in which the cre gene is driven by the MyoD promoter and core enhancer, which directs the early activation of MyoD. Specificity of cre expression and efficiency of recombination was determined by monitoring reporter gene expression after crossing to the Cre-dependent reporter lines, R26R and Z/AP. Efficient labeling of embryonic and fetal myoblasts and muscle fibers was observed, with timing that was similar (branchial arches and limb buds) or slightly delayed (myotomes) relative to the endogenous MyoD gene. In satellite cell cultures, a strict concordance between MyoD protein and reporter gene expression was observed, demonstrating the muscle specificity and efficiency of Cre-mediated recombination. Nascent muscle fibers were labeled following injury of adult muscle, indicating recombination in satellite cells or their daughters in vivo.

Authors+Show Affiliations

Center for Regenerative Biology, Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269-4243, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15729689

Citation

Chen, Jennifer C J., et al. "MyoD-cre Transgenic Mice: a Model for Conditional Mutagenesis and Lineage Tracing of Skeletal Muscle." Genesis (New York, N.Y. : 2000), vol. 41, no. 3, 2005, pp. 116-21.
Chen JC, Mortimer J, Marley J, et al. MyoD-cre transgenic mice: a model for conditional mutagenesis and lineage tracing of skeletal muscle. Genesis. 2005;41(3):116-21.
Chen, J. C., Mortimer, J., Marley, J., & Goldhamer, D. J. (2005). MyoD-cre transgenic mice: a model for conditional mutagenesis and lineage tracing of skeletal muscle. Genesis (New York, N.Y. : 2000), 41(3), 116-21.
Chen JC, et al. MyoD-cre Transgenic Mice: a Model for Conditional Mutagenesis and Lineage Tracing of Skeletal Muscle. Genesis. 2005;41(3):116-21. PubMed PMID: 15729689.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - MyoD-cre transgenic mice: a model for conditional mutagenesis and lineage tracing of skeletal muscle. AU - Chen,Jennifer C J, AU - Mortimer,Justin, AU - Marley,Jason, AU - Goldhamer,David J, PY - 2005/2/25/pubmed PY - 2005/7/15/medline PY - 2005/2/25/entrez SP - 116 EP - 21 JF - Genesis (New York, N.Y. : 2000) JO - Genesis VL - 41 IS - 3 N2 - The Cre-loxP recombination system has been used to great advantage in vivo for conditional gene targeting, lineage tracing, and other applications. To express cre in skeletal myoblasts and muscle fibers, we utilized the well-characterized transcriptional regulatory regions of the muscle determination gene, MyoD. Transgenic mouse lines were produced (F3/-2.5cre) in which the cre gene is driven by the MyoD promoter and core enhancer, which directs the early activation of MyoD. Specificity of cre expression and efficiency of recombination was determined by monitoring reporter gene expression after crossing to the Cre-dependent reporter lines, R26R and Z/AP. Efficient labeling of embryonic and fetal myoblasts and muscle fibers was observed, with timing that was similar (branchial arches and limb buds) or slightly delayed (myotomes) relative to the endogenous MyoD gene. In satellite cell cultures, a strict concordance between MyoD protein and reporter gene expression was observed, demonstrating the muscle specificity and efficiency of Cre-mediated recombination. Nascent muscle fibers were labeled following injury of adult muscle, indicating recombination in satellite cells or their daughters in vivo. SN - 1526-954X UR - https://www.unboundmedicine.com/medline/citation/15729689/MyoD_cre_transgenic_mice:_a_model_for_conditional_mutagenesis_and_lineage_tracing_of_skeletal_muscle_ L2 - https://doi.org/10.1002/gene.20104 DB - PRIME DP - Unbound Medicine ER -