Tags

Type your tag names separated by a space and hit enter

Ontogeny of CD4+CD25+ regulatory/suppressor T cells in human fetuses.
Blood. 2005 Jun 15; 105(12):4715-21.Blood

Abstract

Little is known about the ontogeny of naturally occurring CD4(+)CD25(+) regulatory/suppressor T cells that play a major role in maintaining self-tolerance in mice and humans. In rodents, thymectomy on day 3 of life leads to multiple organ-specific autoimmune diseases that can be prevented by adoptive transfer of regulatory T cells, suggesting their neonatal development. We investigated regulatory T-cell ontogeny in 11 human fetuses. Together with the first mature T cells, thymic CD4(+)CD25(+) cells were detected as early as 13 weeks of gestation. Thymic CD25(+) cells appeared to be positively selected at the CD4(+)CD8(+)CD3(hi) differentiation stage, as assessed by CD1a and CD69 expression. The proportion of thymic CD4(+)CD25(+) cells appeared quite stable with age, around 6% to 7%, similar to the proportion observed in infant thymi. Extrathymic CD4(+)CD25(+) T cells could hardly be detected at 13 weeks of gestation but were present from week 14 onwards. As adult regulatory T cells, purified CD4(+)CD25(+) fetal cells were anergic and suppressed T-cell proliferative responses; they expressed intracellular cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) and Foxp3 mRNA. Altogether, our results indicate that the generation of regulatory/suppressor T cells is consubstantial to the generation of a functional and self-tolerant immune system.

Authors+Show Affiliations

Laboratoire de Biologie et Thérapeutique des Pathologies Immunitaires, Université Pierre et Marie Curie /Centre National de la Recherche Scientifique, Unité Mixte de Recherche, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15731180

Citation

Darrasse-Jèze, Guillaume, et al. "Ontogeny of CD4+CD25+ Regulatory/suppressor T Cells in Human Fetuses." Blood, vol. 105, no. 12, 2005, pp. 4715-21.
Darrasse-Jèze G, Marodon G, Salomon BL, et al. Ontogeny of CD4+CD25+ regulatory/suppressor T cells in human fetuses. Blood. 2005;105(12):4715-21.
Darrasse-Jèze, G., Marodon, G., Salomon, B. L., Catala, M., & Klatzmann, D. (2005). Ontogeny of CD4+CD25+ regulatory/suppressor T cells in human fetuses. Blood, 105(12), 4715-21.
Darrasse-Jèze G, et al. Ontogeny of CD4+CD25+ Regulatory/suppressor T Cells in Human Fetuses. Blood. 2005 Jun 15;105(12):4715-21. PubMed PMID: 15731180.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ontogeny of CD4+CD25+ regulatory/suppressor T cells in human fetuses. AU - Darrasse-Jèze,Guillaume, AU - Marodon,Gilles, AU - Salomon,Benoît L, AU - Catala,Martin, AU - Klatzmann,David, Y1 - 2005/02/24/ PY - 2005/2/26/pubmed PY - 2005/7/14/medline PY - 2005/2/26/entrez SP - 4715 EP - 21 JF - Blood JO - Blood VL - 105 IS - 12 N2 - Little is known about the ontogeny of naturally occurring CD4(+)CD25(+) regulatory/suppressor T cells that play a major role in maintaining self-tolerance in mice and humans. In rodents, thymectomy on day 3 of life leads to multiple organ-specific autoimmune diseases that can be prevented by adoptive transfer of regulatory T cells, suggesting their neonatal development. We investigated regulatory T-cell ontogeny in 11 human fetuses. Together with the first mature T cells, thymic CD4(+)CD25(+) cells were detected as early as 13 weeks of gestation. Thymic CD25(+) cells appeared to be positively selected at the CD4(+)CD8(+)CD3(hi) differentiation stage, as assessed by CD1a and CD69 expression. The proportion of thymic CD4(+)CD25(+) cells appeared quite stable with age, around 6% to 7%, similar to the proportion observed in infant thymi. Extrathymic CD4(+)CD25(+) T cells could hardly be detected at 13 weeks of gestation but were present from week 14 onwards. As adult regulatory T cells, purified CD4(+)CD25(+) fetal cells were anergic and suppressed T-cell proliferative responses; they expressed intracellular cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) and Foxp3 mRNA. Altogether, our results indicate that the generation of regulatory/suppressor T cells is consubstantial to the generation of a functional and self-tolerant immune system. SN - 0006-4971 UR - https://www.unboundmedicine.com/medline/citation/15731180/Ontogeny_of_CD4+CD25+_regulatory/suppressor_T_cells_in_human_fetuses_ L2 - https://linkinghub.elsevier.com/retrieve/pii/2004-10-4051 DB - PRIME DP - Unbound Medicine ER -