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GC/MS profiling of gamma-hydroxybutyrate and precursors in various animal tissues using automatic solid-phase extraction. Preliminary investigations of its potential interest in postmortem interval determination.
Anal Chem. 2005 Mar 01; 77(5):1354-60.AC

Abstract

To quantify gamma-hydroxybutyrate (GHB) and its physiological metabolites, gamma-aminobutyric acid (GABA), 1,4-butanediol (1,4-BD), and gamma-butyrolactone (GBL) in various animal tissues (kidney, muscle, heart, liver, blood, brain cortex, thalamus, hypothalamus, hippocampus, or pons), an original gas chromatographic/mass spectrometric method with a automated solid-phase extraction by Oasis MCX cartridges on a Gilson Aspec Xli was developed. Using such apparatus allowed the limit of detection (LOD) of target compounds to be significantly lowered (LOD: 0.027, 0.025, and 5.7 microg/mL for GHB, 1,4-BD, and GABA, respectively, in 200 microL or microg of sample). After validation of each analytical step, the satisfactory performances of the apparatus in conjunction with the rapidity and ease of the extraction step make it suitable for simultaneous assay of GHB, 1,4-BD, GBL, and GABA. The method was used to test the correlation between GHB levels in tissues obtained at different times after death of male Sprague-Dawley rats and the postmortem interval. Preliminary results show a linear increase of GHB levels in relation to time of death in thoracic blood and central nervous system of animals kept at 15 and 20 degrees C.

Authors+Show Affiliations

Laboratoire de Toxicologie, Faculté de Pharmacie, Institut Régional de Médecine Légale, CHU G. Montpied, 63000 Clermont-Ferrand, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15732918

Citation

Richard, Damien, et al. "GC/MS Profiling of Gamma-hydroxybutyrate and Precursors in Various Animal Tissues Using Automatic Solid-phase Extraction. Preliminary Investigations of Its Potential Interest in Postmortem Interval Determination." Analytical Chemistry, vol. 77, no. 5, 2005, pp. 1354-60.
Richard D, Ling B, Authier N, et al. GC/MS profiling of gamma-hydroxybutyrate and precursors in various animal tissues using automatic solid-phase extraction. Preliminary investigations of its potential interest in postmortem interval determination. Anal Chem. 2005;77(5):1354-60.
Richard, D., Ling, B., Authier, N., Faict, T. W., Eschalier, A., & Coudoré, F. (2005). GC/MS profiling of gamma-hydroxybutyrate and precursors in various animal tissues using automatic solid-phase extraction. Preliminary investigations of its potential interest in postmortem interval determination. Analytical Chemistry, 77(5), 1354-60.
Richard D, et al. GC/MS Profiling of Gamma-hydroxybutyrate and Precursors in Various Animal Tissues Using Automatic Solid-phase Extraction. Preliminary Investigations of Its Potential Interest in Postmortem Interval Determination. Anal Chem. 2005 Mar 1;77(5):1354-60. PubMed PMID: 15732918.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - GC/MS profiling of gamma-hydroxybutyrate and precursors in various animal tissues using automatic solid-phase extraction. Preliminary investigations of its potential interest in postmortem interval determination. AU - Richard,Damien, AU - Ling,Bing, AU - Authier,Nicolas, AU - Faict,Thierry W, AU - Eschalier,Alain, AU - Coudoré,François, PY - 2005/3/1/pubmed PY - 2007/1/18/medline PY - 2005/3/1/entrez SP - 1354 EP - 60 JF - Analytical chemistry JO - Anal Chem VL - 77 IS - 5 N2 - To quantify gamma-hydroxybutyrate (GHB) and its physiological metabolites, gamma-aminobutyric acid (GABA), 1,4-butanediol (1,4-BD), and gamma-butyrolactone (GBL) in various animal tissues (kidney, muscle, heart, liver, blood, brain cortex, thalamus, hypothalamus, hippocampus, or pons), an original gas chromatographic/mass spectrometric method with a automated solid-phase extraction by Oasis MCX cartridges on a Gilson Aspec Xli was developed. Using such apparatus allowed the limit of detection (LOD) of target compounds to be significantly lowered (LOD: 0.027, 0.025, and 5.7 microg/mL for GHB, 1,4-BD, and GABA, respectively, in 200 microL or microg of sample). After validation of each analytical step, the satisfactory performances of the apparatus in conjunction with the rapidity and ease of the extraction step make it suitable for simultaneous assay of GHB, 1,4-BD, GBL, and GABA. The method was used to test the correlation between GHB levels in tissues obtained at different times after death of male Sprague-Dawley rats and the postmortem interval. Preliminary results show a linear increase of GHB levels in relation to time of death in thoracic blood and central nervous system of animals kept at 15 and 20 degrees C. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/15732918/GC/MS_profiling_of_gamma_hydroxybutyrate_and_precursors_in_various_animal_tissues_using_automatic_solid_phase_extraction__Preliminary_investigations_of_its_potential_interest_in_postmortem_interval_determination_ L2 - https://doi.org/10.1021/ac048471h DB - PRIME DP - Unbound Medicine ER -