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The influence of hypothermia on P2 receptor-mediated responses of frog skeletal muscle.
Eur J Pharmacol. 2005 Feb 21; 509(2-3):187-93.EJ

Abstract

The contractile responses of isolated Rana ridibunda frog sartorius muscle contractions evoked by electrical field stimulation (EFS) were studied at three temperature conditions of 17, 22 and 27 degrees C. Temperature-dependent increase of muscle contractility was found. ATP (10-100 microM) concentration dependently inhibited the electrical field stimulation-evoked contractions of sartorius muscle at all three temperatures; this effect was significantly more prominent at a temperature of 17 degrees C than at other two temperatures. Adenosine (100 microM) also caused inhibition of electrical field stimulation-evoked contractions which was statistically identical at all three temperature conditions tested. A P2 receptor antagonist, pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS, 10 microM) reduced the inhibitory effect of ATP at all three temperatures but did not affect inhibitory action of adenosine. In contrast, 8-(p-sulfophenyl)theophylline (8-SPT, 100 microM), a nonselective P1 receptor antagonist, abolished inhibitory effects of adenosine at all three temperature conditions but did not antagonize inhibition caused by ATP. In electrophysiological experiments, ATP (100 microM) and adenosine (100 microM) temperature dependently reduced end-plate currents recorded in sartorius neuromuscular junction by voltage-clamp technique. The inhibitory effects of both agonists were enhanced with the decrease of temperature. 8-SPT (100 microM) abolished the inhibitory effect of adenosine but not ATP on end-plate currents. Suramin (100 microM), a nonselective P2 receptor antagonist, inhibited the action of ATP but not adenosine, while PPADS (10 microM) had no influence on the effects of either ATP or adenosine. It is concluded from this study that the effectiveness of P2 receptor-mediated inhibition of frog skeletal muscle contraction in contrast to that of adenosine is dependent on the temperature conditions.

Authors+Show Affiliations

Department of Pharmacology, Kazan State Medical University, 49 Butlerov Street, Kazan 420012, Russia. airatziganshin@yahoo.co.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15733555

Citation

Ziganshin, Airat U., et al. "The Influence of Hypothermia On P2 Receptor-mediated Responses of Frog Skeletal Muscle." European Journal of Pharmacology, vol. 509, no. 2-3, 2005, pp. 187-93.
Ziganshin AU, Kamaliev RR, Grishin SN, et al. The influence of hypothermia on P2 receptor-mediated responses of frog skeletal muscle. Eur J Pharmacol. 2005;509(2-3):187-93.
Ziganshin, A. U., Kamaliev, R. R., Grishin, S. N., Ziganshina, L. E., Zefirov, A. L., & Burnstock, G. (2005). The influence of hypothermia on P2 receptor-mediated responses of frog skeletal muscle. European Journal of Pharmacology, 509(2-3), 187-93.
Ziganshin AU, et al. The Influence of Hypothermia On P2 Receptor-mediated Responses of Frog Skeletal Muscle. Eur J Pharmacol. 2005 Feb 21;509(2-3):187-93. PubMed PMID: 15733555.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The influence of hypothermia on P2 receptor-mediated responses of frog skeletal muscle. AU - Ziganshin,Airat U, AU - Kamaliev,Rafis R, AU - Grishin,Sergey N, AU - Ziganshina,Lilia E, AU - Zefirov,Andrey L, AU - Burnstock,Geoffrey, PY - 2004/11/09/received PY - 2004/11/15/accepted PY - 2005/3/1/pubmed PY - 2005/6/15/medline PY - 2005/3/1/entrez SP - 187 EP - 93 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 509 IS - 2-3 N2 - The contractile responses of isolated Rana ridibunda frog sartorius muscle contractions evoked by electrical field stimulation (EFS) were studied at three temperature conditions of 17, 22 and 27 degrees C. Temperature-dependent increase of muscle contractility was found. ATP (10-100 microM) concentration dependently inhibited the electrical field stimulation-evoked contractions of sartorius muscle at all three temperatures; this effect was significantly more prominent at a temperature of 17 degrees C than at other two temperatures. Adenosine (100 microM) also caused inhibition of electrical field stimulation-evoked contractions which was statistically identical at all three temperature conditions tested. A P2 receptor antagonist, pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS, 10 microM) reduced the inhibitory effect of ATP at all three temperatures but did not affect inhibitory action of adenosine. In contrast, 8-(p-sulfophenyl)theophylline (8-SPT, 100 microM), a nonselective P1 receptor antagonist, abolished inhibitory effects of adenosine at all three temperature conditions but did not antagonize inhibition caused by ATP. In electrophysiological experiments, ATP (100 microM) and adenosine (100 microM) temperature dependently reduced end-plate currents recorded in sartorius neuromuscular junction by voltage-clamp technique. The inhibitory effects of both agonists were enhanced with the decrease of temperature. 8-SPT (100 microM) abolished the inhibitory effect of adenosine but not ATP on end-plate currents. Suramin (100 microM), a nonselective P2 receptor antagonist, inhibited the action of ATP but not adenosine, while PPADS (10 microM) had no influence on the effects of either ATP or adenosine. It is concluded from this study that the effectiveness of P2 receptor-mediated inhibition of frog skeletal muscle contraction in contrast to that of adenosine is dependent on the temperature conditions. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/15733555/The_influence_of_hypothermia_on_P2_receptor_mediated_responses_of_frog_skeletal_muscle_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(04)01329-9 DB - PRIME DP - Unbound Medicine ER -