Tags

Type your tag names separated by a space and hit enter

Neurotensin activation of the NTR1 on spinally-projecting serotonergic neurons in the rostral ventromedial medulla is antinociceptive.
Pain. 2005 Mar; 114(1-2):285-94.PAIN

Abstract

Microinjection of neurotensin (NT) in the rostral ventromedial medulla (RVM) produces dose-dependent antinociception. The NTR1 (Neurotensin Receptor Subtype 1) may mediate part of this response, however definitive evidence is lacking, and the spinal mediators of NTR1-induced antinociception are unknown. In the present study, we used immunohistochemical techniques to show that the NTR1, but not the NTR2 is expressed by spinally projecting serotonergic neurons of the RVM. We also show that microinjection of NT or the NTR1-selective agonist PD149163 in the RVM both produce dose-dependent antinociception in the tail-flick test that is blocked by the NTR1-selective antagonist SR48692. The antinociception produced by NT or PD149163 is also blocked by intrathecal administration of the non-selective serotonergic receptor antagonist methysergide. The results of these experiments provide anatomical and behavioral evidence that activation of NTR1-expressing spinally projecting neurons in the RVM produces antinociception through release of serotonin in the spinal dorsal horn. These results support the conclusion that the NTR1 plays an important role in the central modulation of nociception.

Authors+Show Affiliations

Department of Pharmacology, Carver College of Medicine, The University of Iowa, Iowa City, IA 52242, USA. amber-buhler@uiowa.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15733655

Citation

Buhler, A V., et al. "Neurotensin Activation of the NTR1 On Spinally-projecting Serotonergic Neurons in the Rostral Ventromedial Medulla Is Antinociceptive." Pain, vol. 114, no. 1-2, 2005, pp. 285-94.
Buhler AV, Choi J, Proudfit HK, et al. Neurotensin activation of the NTR1 on spinally-projecting serotonergic neurons in the rostral ventromedial medulla is antinociceptive. Pain. 2005;114(1-2):285-94.
Buhler, A. V., Choi, J., Proudfit, H. K., & Gebhart, G. F. (2005). Neurotensin activation of the NTR1 on spinally-projecting serotonergic neurons in the rostral ventromedial medulla is antinociceptive. Pain, 114(1-2), 285-94.
Buhler AV, et al. Neurotensin Activation of the NTR1 On Spinally-projecting Serotonergic Neurons in the Rostral Ventromedial Medulla Is Antinociceptive. Pain. 2005;114(1-2):285-94. PubMed PMID: 15733655.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neurotensin activation of the NTR1 on spinally-projecting serotonergic neurons in the rostral ventromedial medulla is antinociceptive. AU - Buhler,A V, AU - Choi,J, AU - Proudfit,H K, AU - Gebhart,G F, Y1 - 2005/01/26/ PY - 2004/08/20/received PY - 2004/12/07/revised PY - 2004/12/20/accepted PY - 2005/3/1/pubmed PY - 2005/5/17/medline PY - 2005/3/1/entrez SP - 285 EP - 94 JF - Pain JO - Pain VL - 114 IS - 1-2 N2 - Microinjection of neurotensin (NT) in the rostral ventromedial medulla (RVM) produces dose-dependent antinociception. The NTR1 (Neurotensin Receptor Subtype 1) may mediate part of this response, however definitive evidence is lacking, and the spinal mediators of NTR1-induced antinociception are unknown. In the present study, we used immunohistochemical techniques to show that the NTR1, but not the NTR2 is expressed by spinally projecting serotonergic neurons of the RVM. We also show that microinjection of NT or the NTR1-selective agonist PD149163 in the RVM both produce dose-dependent antinociception in the tail-flick test that is blocked by the NTR1-selective antagonist SR48692. The antinociception produced by NT or PD149163 is also blocked by intrathecal administration of the non-selective serotonergic receptor antagonist methysergide. The results of these experiments provide anatomical and behavioral evidence that activation of NTR1-expressing spinally projecting neurons in the RVM produces antinociception through release of serotonin in the spinal dorsal horn. These results support the conclusion that the NTR1 plays an important role in the central modulation of nociception. SN - 0304-3959 UR - https://www.unboundmedicine.com/medline/citation/15733655/Neurotensin_activation_of_the_NTR1_on_spinally_projecting_serotonergic_neurons_in_the_rostral_ventromedial_medulla_is_antinociceptive_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3959(04)00618-9 DB - PRIME DP - Unbound Medicine ER -