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Development of cholinergic amacrine cells is visual activity-dependent in the postnatal mouse retina.
J Comp Neurol 2005; 484(3):331-43JC

Abstract

In the present study, we used immunocytochemistry to study the temporal and spatial arrangement of mouse cholinergic amacrine cells during postnatal retinal development under normal light/dark cycles and during visual deprivation. Choline acetyltransferase (ChAT)-immunolabeled cells were detected in the neuroblastic layer (NBL) and in the ganglion cell layer (GCL) at postnatal day 0 (P0). Between P3-5, two characteristic cholinergic bands were clearly identified in the inner plexiform layer (IPL). The signal intensity of somas and processes progressively increased over the first 2 postnatal weeks. Around eye opening at P12, cholinergic neurons were mature-like. This early developmental process was not altered by visual deprivation. After eye opening, the space between the two cholinergic bands increased continuously and the spatial regularity index changed constantly, indicating that the cholinergic neurons possibly underwent refinement during later postnatal development. The changes occurring following eye opening were retarded by visual deprivation. The morphologies of photoreceptors, horizontal cells, recoverin-positive OFF-cone bipolar cells, rod bipolar cells, dopaminergic amacrine cells, and Müller cells appeared normal. Their stratification in the outer plexiform layer (OPL) and the IPL was not affected by visual deprivation. However, glial cells grew vertically across the entire thickness of dark-reared retinas. Our results suggest that the development of cholinergic neurons before eye opening is independent of the lighting conditions. Their development after eye opening is greatly impeded by visual deprivation. This visual activity-dependent phase of development may be a critical period for the maturation and synaptic wiring of cholinergic amacrine cells in the mammalian retina.

Authors+Show Affiliations

Cullen Eye Institute, Baylor College of Medicine, Houston, Texas 77030, USA. jianz@bcm.tmc.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15739235

Citation

Zhang, Jian, et al. "Development of Cholinergic Amacrine Cells Is Visual Activity-dependent in the Postnatal Mouse Retina." The Journal of Comparative Neurology, vol. 484, no. 3, 2005, pp. 331-43.
Zhang J, Yang Z, Wu SM. Development of cholinergic amacrine cells is visual activity-dependent in the postnatal mouse retina. J Comp Neurol. 2005;484(3):331-43.
Zhang, J., Yang, Z., & Wu, S. M. (2005). Development of cholinergic amacrine cells is visual activity-dependent in the postnatal mouse retina. The Journal of Comparative Neurology, 484(3), pp. 331-43.
Zhang J, Yang Z, Wu SM. Development of Cholinergic Amacrine Cells Is Visual Activity-dependent in the Postnatal Mouse Retina. J Comp Neurol. 2005 Apr 11;484(3):331-43. PubMed PMID: 15739235.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of cholinergic amacrine cells is visual activity-dependent in the postnatal mouse retina. AU - Zhang,Jian, AU - Yang,Zhuo, AU - Wu,Samuel M, PY - 2005/3/2/pubmed PY - 2005/5/17/medline PY - 2005/3/2/entrez SP - 331 EP - 43 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 484 IS - 3 N2 - In the present study, we used immunocytochemistry to study the temporal and spatial arrangement of mouse cholinergic amacrine cells during postnatal retinal development under normal light/dark cycles and during visual deprivation. Choline acetyltransferase (ChAT)-immunolabeled cells were detected in the neuroblastic layer (NBL) and in the ganglion cell layer (GCL) at postnatal day 0 (P0). Between P3-5, two characteristic cholinergic bands were clearly identified in the inner plexiform layer (IPL). The signal intensity of somas and processes progressively increased over the first 2 postnatal weeks. Around eye opening at P12, cholinergic neurons were mature-like. This early developmental process was not altered by visual deprivation. After eye opening, the space between the two cholinergic bands increased continuously and the spatial regularity index changed constantly, indicating that the cholinergic neurons possibly underwent refinement during later postnatal development. The changes occurring following eye opening were retarded by visual deprivation. The morphologies of photoreceptors, horizontal cells, recoverin-positive OFF-cone bipolar cells, rod bipolar cells, dopaminergic amacrine cells, and Müller cells appeared normal. Their stratification in the outer plexiform layer (OPL) and the IPL was not affected by visual deprivation. However, glial cells grew vertically across the entire thickness of dark-reared retinas. Our results suggest that the development of cholinergic neurons before eye opening is independent of the lighting conditions. Their development after eye opening is greatly impeded by visual deprivation. This visual activity-dependent phase of development may be a critical period for the maturation and synaptic wiring of cholinergic amacrine cells in the mammalian retina. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/15739235/Development_of_cholinergic_amacrine_cells_is_visual_activity_dependent_in_the_postnatal_mouse_retina_ L2 - https://doi.org/10.1002/cne.20470 DB - PRIME DP - Unbound Medicine ER -