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[Separation and determination of plant growth regulators by capillary micellar electrokinetic chromatography].
Se Pu. 1997 Nov; 15(6):482-5.SP

Abstract

It is important for separation and determination of plant growth regulators in the research of plant physiology. A study of the separation of five plant growth regulators, such as abscisic acid (ABA), gibberellic acid (GA), indole-butyric acid (IBA), indole acetic acid (IAA) and naphthaleneacetic (NAA) by micellar electrokinetic chromatography (MEKC) is presented. The effects of pH, SDS concentration, applied voltage and organic additives on migration behavior were investigated. Results showed that satisfactory separation was achieved on optimization of electrophoretic condition: 80 mmol/L SDS, 10 mmol/L borate-phosphate, 5% isopropanol, 18 kV applied voltage, pH 8.0. The SDS concentration and organic additives played an important role in separating plant growth regulators, and the applied voltage and pH gave less effect. The detection limit of ABA, GA, IBA, IAA and NAA were 5.0 x 10(-3), 3.0 x 10(-3), 5.8 x 10(-4), 1.5 x 10(-4) and 1.4 x 10(-4) g/L respectively. Increasing of the SDS concentration or organic additives would change separation factor (alpha) and result an improvement of the resolution of difficult separation pairs ABA-GA and IAA-NAA.

Authors+Show Affiliations

Department of Chemistry, South China Normal University, Guangzhou, 510631.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15739327

Citation

Yuan, M, et al. "[Separation and Determination of Plant Growth Regulators By Capillary Micellar Electrokinetic Chromatography]." Se Pu = Chinese Journal of Chromatography, vol. 15, no. 6, 1997, pp. 482-5.
Yuan M, Zhang M, Kang J, et al. [Separation and determination of plant growth regulators by capillary micellar electrokinetic chromatography]. Se Pu. 1997;15(6):482-5.
Yuan, M., Zhang, M., Kang, J., & Li, J. (1997). [Separation and determination of plant growth regulators by capillary micellar electrokinetic chromatography]. Se Pu = Chinese Journal of Chromatography, 15(6), 482-5.
Yuan M, et al. [Separation and Determination of Plant Growth Regulators By Capillary Micellar Electrokinetic Chromatography]. Se Pu. 1997;15(6):482-5. PubMed PMID: 15739327.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Separation and determination of plant growth regulators by capillary micellar electrokinetic chromatography]. AU - Yuan,M, AU - Zhang,M, AU - Kang,J, AU - Li,J, PY - 2005/3/3/pubmed PY - 2010/4/16/medline PY - 2005/3/3/entrez SP - 482 EP - 5 JF - Se pu = Chinese journal of chromatography JO - Se Pu VL - 15 IS - 6 N2 - It is important for separation and determination of plant growth regulators in the research of plant physiology. A study of the separation of five plant growth regulators, such as abscisic acid (ABA), gibberellic acid (GA), indole-butyric acid (IBA), indole acetic acid (IAA) and naphthaleneacetic (NAA) by micellar electrokinetic chromatography (MEKC) is presented. The effects of pH, SDS concentration, applied voltage and organic additives on migration behavior were investigated. Results showed that satisfactory separation was achieved on optimization of electrophoretic condition: 80 mmol/L SDS, 10 mmol/L borate-phosphate, 5% isopropanol, 18 kV applied voltage, pH 8.0. The SDS concentration and organic additives played an important role in separating plant growth regulators, and the applied voltage and pH gave less effect. The detection limit of ABA, GA, IBA, IAA and NAA were 5.0 x 10(-3), 3.0 x 10(-3), 5.8 x 10(-4), 1.5 x 10(-4) and 1.4 x 10(-4) g/L respectively. Increasing of the SDS concentration or organic additives would change separation factor (alpha) and result an improvement of the resolution of difficult separation pairs ABA-GA and IAA-NAA. SN - 1000-8713 UR - https://www.unboundmedicine.com/medline/citation/15739327/[Separation_and_determination_of_plant_growth_regulators_by_capillary_micellar_electrokinetic_chromatography]_ DB - PRIME DP - Unbound Medicine ER -