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A cluster of genes located in 1p36 are down-regulated in neuroblastomas with poor prognosis, but not due to CpG island methylation.
Mol Cancer. 2005 Mar 01; 4(1):10.MC

Abstract

BACKGROUND

A common feature of neuroblastoma tumours are partial deletions of the short arm of chromosome 1 (1p-deletions). This is indicative of a neuroblastoma tumour suppressor gene being located in the region. Several groups including our have been studying candidate neuroblastoma genes in the region, but no gene/genes have yet been found that fulfil the criteria for being a neuroblastoma tumour suppressor. Since frequent mutations have not been detected, we have now analyzed the expression and promoter CpG island methylation status of the genes UBE4B, KIF1B, PGD, APITD1, DFFA and PEX14 in the 1p36.22 region in order to find an explanation for a possible down-regulation of this region.

RESULTS

The current study shows that gene transcripts in high stage neuroblastoma tumours are significantly down-regulated compared to those in low stage tumours in the 1p36.22 region. CpG island methylation does not seem to be the mechanism of down-regulation for most of the genes tested, since no methylation was detected in the fragments analyzed. One exception is the CpG island of APITD1. Methylation of this gene is also seen in blood from control individuals and is therefore not believed to participate in tumour development.

CONCLUSION

The genes UBE4B, KIF1B, PGD, APITD1, DFFA and PEX14 are down-regulated in high stage NB tumours, a feature that can not be explained by CpG island methylation.

Authors+Show Affiliations

Department of Clinical Genetics, Institute for the Health of Women and Children, Göteborg University, Sahlgrenska Univ, Hospital-East, SE-41685 Gothenburg, Sweden. helena.caren@clingen.gu.se <helena.caren@clingen.gu.se>No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15740626

Citation

Carén, Helena, et al. "A Cluster of Genes Located in 1p36 Are Down-regulated in Neuroblastomas With Poor Prognosis, but Not Due to CpG Island Methylation." Molecular Cancer, vol. 4, no. 1, 2005, p. 10.
Carén H, Ejeskär K, Fransson S, et al. A cluster of genes located in 1p36 are down-regulated in neuroblastomas with poor prognosis, but not due to CpG island methylation. Mol Cancer. 2005;4(1):10.
Carén, H., Ejeskär, K., Fransson, S., Hesson, L., Latif, F., Sjöberg, R. M., Krona, C., & Martinsson, T. (2005). A cluster of genes located in 1p36 are down-regulated in neuroblastomas with poor prognosis, but not due to CpG island methylation. Molecular Cancer, 4(1), 10.
Carén H, et al. A Cluster of Genes Located in 1p36 Are Down-regulated in Neuroblastomas With Poor Prognosis, but Not Due to CpG Island Methylation. Mol Cancer. 2005 Mar 1;4(1):10. PubMed PMID: 15740626.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A cluster of genes located in 1p36 are down-regulated in neuroblastomas with poor prognosis, but not due to CpG island methylation. AU - Carén,Helena, AU - Ejeskär,Katarina, AU - Fransson,Susanne, AU - Hesson,Luke, AU - Latif,Farida, AU - Sjöberg,Rose-Marie, AU - Krona,Cecilia, AU - Martinsson,Tommy, Y1 - 2005/03/01/ PY - 2004/12/01/received PY - 2005/03/01/accepted PY - 2005/3/3/pubmed PY - 2006/12/12/medline PY - 2005/3/3/entrez SP - 10 EP - 10 JF - Molecular cancer JO - Mol Cancer VL - 4 IS - 1 N2 - BACKGROUND: A common feature of neuroblastoma tumours are partial deletions of the short arm of chromosome 1 (1p-deletions). This is indicative of a neuroblastoma tumour suppressor gene being located in the region. Several groups including our have been studying candidate neuroblastoma genes in the region, but no gene/genes have yet been found that fulfil the criteria for being a neuroblastoma tumour suppressor. Since frequent mutations have not been detected, we have now analyzed the expression and promoter CpG island methylation status of the genes UBE4B, KIF1B, PGD, APITD1, DFFA and PEX14 in the 1p36.22 region in order to find an explanation for a possible down-regulation of this region. RESULTS: The current study shows that gene transcripts in high stage neuroblastoma tumours are significantly down-regulated compared to those in low stage tumours in the 1p36.22 region. CpG island methylation does not seem to be the mechanism of down-regulation for most of the genes tested, since no methylation was detected in the fragments analyzed. One exception is the CpG island of APITD1. Methylation of this gene is also seen in blood from control individuals and is therefore not believed to participate in tumour development. CONCLUSION: The genes UBE4B, KIF1B, PGD, APITD1, DFFA and PEX14 are down-regulated in high stage NB tumours, a feature that can not be explained by CpG island methylation. SN - 1476-4598 UR - https://www.unboundmedicine.com/medline/citation/15740626/A_cluster_of_genes_located_in_1p36_are_down_regulated_in_neuroblastomas_with_poor_prognosis_but_not_due_to_CpG_island_methylation_ L2 - https://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-4-10 DB - PRIME DP - Unbound Medicine ER -