Tags

Type your tag names separated by a space and hit enter

Structural analyses of protoberberine alkaloids in medicine herbs by using ESI-FT-ICR-MS and HPLC-ESI-MS(n).
J Pharm Biomed Anal. 2005 Mar 09; 37(3):437-46.JP

Abstract

The Electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FT-ICR-MS) using sustained off-resonance irradiation (SORI)/collision-induced dissociation (CID) method at high mass resolution has been first applied to investigate the characteristic fragment ions of four protoberberine alkaloids in medicine herbs. The ESI-FT-ICR SORI-CID experiment results demonstrate that the unambiguous elemental composition of fragment ions can be obtained at high mass resolution, then the logical fragmentation pathways of the protoberberine alkaloids has been proposed. The characteristic fragment ions of CID and MS(n) of protoberberine alkaloids have been discussed, which are specific and useful for the identification of some protoberberine alkaloid compounds. Then, the extracts of four kinds of medicine herbs have been analyzed by HPLC-ESI-MS(n). According to these characteristic fragmentation pathways, the retention time (t(R)) of HPLC and mass spectra of product ion, the structures of six kinds of protoberberine alkaloids have been identified. And, in the present paper, the selected ion monitoring (SIM) method has been used to separate and identify the alkaloid isomers.

Authors+Show Affiliations

Laboratory of New Drug Research and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15740901

Citation

Wu, Wei, et al. "Structural Analyses of Protoberberine Alkaloids in Medicine Herbs By Using ESI-FT-ICR-MS and HPLC-ESI-MS(n)." Journal of Pharmaceutical and Biomedical Analysis, vol. 37, no. 3, 2005, pp. 437-46.
Wu W, Song F, Yan C, et al. Structural analyses of protoberberine alkaloids in medicine herbs by using ESI-FT-ICR-MS and HPLC-ESI-MS(n). J Pharm Biomed Anal. 2005;37(3):437-46.
Wu, W., Song, F., Yan, C., Liu, Z., & Liu, S. (2005). Structural analyses of protoberberine alkaloids in medicine herbs by using ESI-FT-ICR-MS and HPLC-ESI-MS(n). Journal of Pharmaceutical and Biomedical Analysis, 37(3), 437-46.
Wu W, et al. Structural Analyses of Protoberberine Alkaloids in Medicine Herbs By Using ESI-FT-ICR-MS and HPLC-ESI-MS(n). J Pharm Biomed Anal. 2005 Mar 9;37(3):437-46. PubMed PMID: 15740901.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structural analyses of protoberberine alkaloids in medicine herbs by using ESI-FT-ICR-MS and HPLC-ESI-MS(n). AU - Wu,Wei, AU - Song,Fengrui, AU - Yan,Cunyu, AU - Liu,Zhiqiang, AU - Liu,Shuying, Y1 - 2004/12/22/ PY - 2004/09/15/received PY - 2004/11/04/revised PY - 2004/11/13/accepted PY - 2005/3/3/pubmed PY - 2005/8/10/medline PY - 2005/3/3/entrez SP - 437 EP - 46 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 37 IS - 3 N2 - The Electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FT-ICR-MS) using sustained off-resonance irradiation (SORI)/collision-induced dissociation (CID) method at high mass resolution has been first applied to investigate the characteristic fragment ions of four protoberberine alkaloids in medicine herbs. The ESI-FT-ICR SORI-CID experiment results demonstrate that the unambiguous elemental composition of fragment ions can be obtained at high mass resolution, then the logical fragmentation pathways of the protoberberine alkaloids has been proposed. The characteristic fragment ions of CID and MS(n) of protoberberine alkaloids have been discussed, which are specific and useful for the identification of some protoberberine alkaloid compounds. Then, the extracts of four kinds of medicine herbs have been analyzed by HPLC-ESI-MS(n). According to these characteristic fragmentation pathways, the retention time (t(R)) of HPLC and mass spectra of product ion, the structures of six kinds of protoberberine alkaloids have been identified. And, in the present paper, the selected ion monitoring (SIM) method has been used to separate and identify the alkaloid isomers. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/15740901/Structural_analyses_of_protoberberine_alkaloids_in_medicine_herbs_by_using_ESI_FT_ICR_MS_and_HPLC_ESI_MS_n__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(04)00607-7 DB - PRIME DP - Unbound Medicine ER -