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Novel planar glucose biosensors for continuous monitoring use.
Biosens Bioelectron. 2005 Apr 15; 20(10):1993-2000.BB

Abstract

Novel planar glucose biosensors to be used for continuous monitoring have been developed. The electrodes are produced with the "screen printing" technique, and present a high degree of reproducibility together with a low cost and the possibility of mass production. Prior to enzyme immobilisation, electrodes are chemically modified with ferric hexacyanoferrate (Prussian Blue). This allows the detection of the hydrogen peroxide produced by the enzymatic reaction catalysed by GOD, at low applied potential (ca. 0.0 V versus Ag/AgCl), highly limiting any electrochemical interferences. The layer of Prussian Blue (PB) showed a high stability at the working conditions (pH 7.4) and also after 1 year of storage dry at RT, no loss of activity was observed. The assembled glucose biosensors, showed high sensitivity towards glucose together with a long-term operational and storage stability. In a continuous flow system, with all the analytical parameters optimised, the glucose biosensors detected glucose concentration as low as 0.025 mM with a linear range up to 1.0mM. These probes were also tested over 50-60 h in a continuous flow mode to evaluate their operational stability. A 0.5 mM concentration of glucose was continuously fluxed into a biosensor wall-jet cell and the current due to the hydrogen peroxide reduction was continuously monitored. After 50-60 h, the drift of the signal observed was around 30%. Because of their high stability, these sensors suggest the possibility of using such biosensors, in conjunction with a microdialysis probe, for a continuous monitoring of glucose for clinical purposes.

Authors+Show Affiliations

Ricci F
Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy. danila.moscone@uniroma2.it
Moscone D
No affiliation info available
Tuta CS
No affiliation info available
Palleschi G
No affiliation info available
Amine A
No affiliation info available
Poscia A
No affiliation info available
Valgimigli F
No affiliation info available
Messeri D
No affiliation info available

MeSH

Biosensing TechniquesBlood GlucoseBlood Glucose Self-MonitoringElectrochemistryElectrodesEquipment DesignEquipment Failure AnalysisFeasibility StudiesFerrocyanidesGlucoseGlucose OxidaseHumansReproducibility of ResultsSensitivity and Specificity

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

15741068

Citation

Ricci, F, et al. "Novel Planar Glucose Biosensors for Continuous Monitoring Use." Biosensors & Bioelectronics, vol. 20, no. 10, 2005, pp. 1993-2000.
Ricci F, Moscone D, Tuta CS, et al. Novel planar glucose biosensors for continuous monitoring use. Biosens Bioelectron. 2005;20(10):1993-2000.
Ricci, F., Moscone, D., Tuta, C. S., Palleschi, G., Amine, A., Poscia, A., Valgimigli, F., & Messeri, D. (2005). Novel planar glucose biosensors for continuous monitoring use. Biosensors & Bioelectronics, 20(10), 1993-2000.
Ricci F, et al. Novel Planar Glucose Biosensors for Continuous Monitoring Use. Biosens Bioelectron. 2005 Apr 15;20(10):1993-2000. PubMed PMID: 15741068.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel planar glucose biosensors for continuous monitoring use. AU - Ricci,F, AU - Moscone,D, AU - Tuta,C S, AU - Palleschi,G, AU - Amine,A, AU - Poscia,A, AU - Valgimigli,F, AU - Messeri,D, PY - 2004/06/18/received PY - 2004/08/30/revised PY - 2004/09/03/accepted PY - 2005/3/3/pubmed PY - 2005/8/16/medline PY - 2005/3/3/entrez SP - 1993 EP - 2000 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 20 IS - 10 N2 - Novel planar glucose biosensors to be used for continuous monitoring have been developed. The electrodes are produced with the "screen printing" technique, and present a high degree of reproducibility together with a low cost and the possibility of mass production. Prior to enzyme immobilisation, electrodes are chemically modified with ferric hexacyanoferrate (Prussian Blue). This allows the detection of the hydrogen peroxide produced by the enzymatic reaction catalysed by GOD, at low applied potential (ca. 0.0 V versus Ag/AgCl), highly limiting any electrochemical interferences. The layer of Prussian Blue (PB) showed a high stability at the working conditions (pH 7.4) and also after 1 year of storage dry at RT, no loss of activity was observed. The assembled glucose biosensors, showed high sensitivity towards glucose together with a long-term operational and storage stability. In a continuous flow system, with all the analytical parameters optimised, the glucose biosensors detected glucose concentration as low as 0.025 mM with a linear range up to 1.0mM. These probes were also tested over 50-60 h in a continuous flow mode to evaluate their operational stability. A 0.5 mM concentration of glucose was continuously fluxed into a biosensor wall-jet cell and the current due to the hydrogen peroxide reduction was continuously monitored. After 50-60 h, the drift of the signal observed was around 30%. Because of their high stability, these sensors suggest the possibility of using such biosensors, in conjunction with a microdialysis probe, for a continuous monitoring of glucose for clinical purposes. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/15741068/Novel_planar_glucose_biosensors_for_continuous_monitoring_use_ DB - PRIME DP - Unbound Medicine ER -