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Molecular epidemiology of Candida species isolated from urine at an intensive care unit.
Mycoses. 2005 Mar; 48(2):126-31.M

Abstract

Candida spp. has been the leading microorganism isolated from the urine specimens of patients hospitalized at the Anesthesiology and Reanimation intensive care unit (ICU) of Dokuz Eylul University Hospital, Izmir, since 1998. This study was undertaken to investigate the clonal relationship of Candida urine isolates in order to find the mode of spread among the patients. Epidemiological surveillance of 38 Candida albicans, 15 Candida tropicalis and 12 Candida glabrata recovered from the urine specimens of patients who were hospitalized in the ICU between June 11, 2000 and October 15, 2001 was carried out by antifungal susceptibility testing and randomly amplified polymorphic DNA (RAPD) analysis. Two short primers [Cnd3 (5'-CCAGATGCAC-3') and Cnd4 (5'-ACGGTACACT-3')] were used for RAPD. None of the isolates had high minimal inhibitory concentration (MIC) values (>1 microg ml(-1)) against amphotericin B with MIC50 values of 0.5 microg ml(-1), 0.5 microg ml(-1) and 0.125 microg ml(-1) for C. albicans, C. tropicalis and C. glabrata isolates, respectively. However, three C. glabrata isolates were resistant and one C. albicans and five C. glabrata isolates were dose-dependent susceptible (D-DS) to fluconazole. Among C. albicans isolates 19 and 20 patterns were detected with primers Cnd3 and Cnd4, respectively. When primers Cnd3 and Cnd4 were evaluated together, three and four genotypes were identified for C. tropicalis and C. glabrata isolates, respectively. Our results suggest that the source of C. albicans isolates was mostly endogenous. It is difficult to interpret the mode of spread of C. tropicalis and C. glabrata urine isolates as we obtained insufficient banding patterns for these species.

Authors+Show Affiliations

Department of Microbiology and Clinical Microbiology, School of Medicine, Dokuz Eylul University, Izmir, Turkey.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15743431

Citation

Ergon, M C., and Z Gülay. "Molecular Epidemiology of Candida Species Isolated From Urine at an Intensive Care Unit." Mycoses, vol. 48, no. 2, 2005, pp. 126-31.
Ergon MC, Gülay Z. Molecular epidemiology of Candida species isolated from urine at an intensive care unit. Mycoses. 2005;48(2):126-31.
Ergon, M. C., & Gülay, Z. (2005). Molecular epidemiology of Candida species isolated from urine at an intensive care unit. Mycoses, 48(2), 126-31.
Ergon MC, Gülay Z. Molecular Epidemiology of Candida Species Isolated From Urine at an Intensive Care Unit. Mycoses. 2005;48(2):126-31. PubMed PMID: 15743431.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular epidemiology of Candida species isolated from urine at an intensive care unit. AU - Ergon,M C, AU - Gülay,Z, PY - 2005/3/4/pubmed PY - 2005/8/19/medline PY - 2005/3/4/entrez SP - 126 EP - 31 JF - Mycoses JO - Mycoses VL - 48 IS - 2 N2 - Candida spp. has been the leading microorganism isolated from the urine specimens of patients hospitalized at the Anesthesiology and Reanimation intensive care unit (ICU) of Dokuz Eylul University Hospital, Izmir, since 1998. This study was undertaken to investigate the clonal relationship of Candida urine isolates in order to find the mode of spread among the patients. Epidemiological surveillance of 38 Candida albicans, 15 Candida tropicalis and 12 Candida glabrata recovered from the urine specimens of patients who were hospitalized in the ICU between June 11, 2000 and October 15, 2001 was carried out by antifungal susceptibility testing and randomly amplified polymorphic DNA (RAPD) analysis. Two short primers [Cnd3 (5'-CCAGATGCAC-3') and Cnd4 (5'-ACGGTACACT-3')] were used for RAPD. None of the isolates had high minimal inhibitory concentration (MIC) values (>1 microg ml(-1)) against amphotericin B with MIC50 values of 0.5 microg ml(-1), 0.5 microg ml(-1) and 0.125 microg ml(-1) for C. albicans, C. tropicalis and C. glabrata isolates, respectively. However, three C. glabrata isolates were resistant and one C. albicans and five C. glabrata isolates were dose-dependent susceptible (D-DS) to fluconazole. Among C. albicans isolates 19 and 20 patterns were detected with primers Cnd3 and Cnd4, respectively. When primers Cnd3 and Cnd4 were evaluated together, three and four genotypes were identified for C. tropicalis and C. glabrata isolates, respectively. Our results suggest that the source of C. albicans isolates was mostly endogenous. It is difficult to interpret the mode of spread of C. tropicalis and C. glabrata urine isolates as we obtained insufficient banding patterns for these species. SN - 0933-7407 UR - https://www.unboundmedicine.com/medline/citation/15743431/Molecular_epidemiology_of_Candida_species_isolated_from_urine_at_an_intensive_care_unit_ L2 - https://doi.org/10.1111/j.1439-0507.2004.01086.x DB - PRIME DP - Unbound Medicine ER -