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Electricity generation from cysteine in a microbial fuel cell.
Water Res. 2005 Mar; 39(5):942-52.WR

Abstract

In a microbial fuel cell (MFC), power can be generated from the oxidation of organic matter by bacteria at the anode, with reduction of oxygen at the cathode. Proton exchange membranes used in MFCs are permeable to oxygen, resulting in the diffusion of oxygen into the anode chamber. This could either lower power generation by obligate anaerobes or result in the loss in electron donor from aerobic respiration by facultative or other aerobic bacteria. In order to maintain anaerobic conditions in conventional anaerobic laboratory cultures, chemical oxygen scavengers such as cysteine are commonly used. It is shown here that cysteine can serve as a substrate for electricity generation by bacteria in a MFC. A two-chamber MFC containing a proton exchange membrane was inoculated with an anaerobic marine sediment. Over a period of a few weeks, electricity generation gradually increased to a maximum power density of 19 mW/m(2) (700 or 1000 Omega resistor; 385 mg/L of cysteine). Power output increased to 39 mW/m(2) when cysteine concentrations were increased up to 770 mg/L (493 Omega resistor). The use of a more active cathode with Pt- or Pt-Ru, increased the maximum power from 19 to 33 mW/m(2) demonstrating that cathode efficiency limited power generation. Power was always immediately generated upon addition of fresh medium, but initial power levels consistently increased by ca. 30% during the first 24 h. Electron recovery as electricity was 14% based on complete cysteine oxidation, with an additional 14% (28% total) potentially lost to oxygen diffusion through the proton exchange membrane. 16S rRNA-based analysis of the biofilm on the anode of the MFC indicated that the predominant organisms were Shewanella spp. closely related to Shewanella affinis (37% of 16S rRNA gene sequences recovered in clone libraries).

Authors+Show Affiliations

Department of Civil and Environmental Engineering, The Pennsylvania State University, 212 Sackett Bldg, University Park, PA 16802, USA. blogan@psu.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15743641

Citation

Logan, Bruce E., et al. "Electricity Generation From Cysteine in a Microbial Fuel Cell." Water Research, vol. 39, no. 5, 2005, pp. 942-52.
Logan BE, Murano C, Scott K, et al. Electricity generation from cysteine in a microbial fuel cell. Water Res. 2005;39(5):942-52.
Logan, B. E., Murano, C., Scott, K., Gray, N. D., & Head, I. M. (2005). Electricity generation from cysteine in a microbial fuel cell. Water Research, 39(5), 942-52.
Logan BE, et al. Electricity Generation From Cysteine in a Microbial Fuel Cell. Water Res. 2005;39(5):942-52. PubMed PMID: 15743641.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electricity generation from cysteine in a microbial fuel cell. AU - Logan,Bruce E, AU - Murano,Cassandro, AU - Scott,Keith, AU - Gray,Neil D, AU - Head,Ian M, Y1 - 2005/01/04/ PY - 2004/07/28/received PY - 2004/10/18/revised PY - 2004/11/22/accepted PY - 2005/3/4/pubmed PY - 2005/7/16/medline PY - 2005/3/4/entrez SP - 942 EP - 52 JF - Water research JO - Water Res VL - 39 IS - 5 N2 - In a microbial fuel cell (MFC), power can be generated from the oxidation of organic matter by bacteria at the anode, with reduction of oxygen at the cathode. Proton exchange membranes used in MFCs are permeable to oxygen, resulting in the diffusion of oxygen into the anode chamber. This could either lower power generation by obligate anaerobes or result in the loss in electron donor from aerobic respiration by facultative or other aerobic bacteria. In order to maintain anaerobic conditions in conventional anaerobic laboratory cultures, chemical oxygen scavengers such as cysteine are commonly used. It is shown here that cysteine can serve as a substrate for electricity generation by bacteria in a MFC. A two-chamber MFC containing a proton exchange membrane was inoculated with an anaerobic marine sediment. Over a period of a few weeks, electricity generation gradually increased to a maximum power density of 19 mW/m(2) (700 or 1000 Omega resistor; 385 mg/L of cysteine). Power output increased to 39 mW/m(2) when cysteine concentrations were increased up to 770 mg/L (493 Omega resistor). The use of a more active cathode with Pt- or Pt-Ru, increased the maximum power from 19 to 33 mW/m(2) demonstrating that cathode efficiency limited power generation. Power was always immediately generated upon addition of fresh medium, but initial power levels consistently increased by ca. 30% during the first 24 h. Electron recovery as electricity was 14% based on complete cysteine oxidation, with an additional 14% (28% total) potentially lost to oxygen diffusion through the proton exchange membrane. 16S rRNA-based analysis of the biofilm on the anode of the MFC indicated that the predominant organisms were Shewanella spp. closely related to Shewanella affinis (37% of 16S rRNA gene sequences recovered in clone libraries). SN - 0043-1354 UR - https://www.unboundmedicine.com/medline/citation/15743641/Electricity_generation_from_cysteine_in_a_microbial_fuel_cell_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0043-1354(04)00530-5 DB - PRIME DP - Unbound Medicine ER -