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CpxR/OmpR interplay regulates curli gene expression in response to osmolarity in Escherichia coli.
J Bacteriol. 2005 Mar; 187(6):2038-49.JB

Abstract

Curli fibers could be described as a virulence factor able to confer adherence properties to both abiotic and eukaryotic surfaces. The ability to adapt rapidly to changing environmental conditions through signal transduction pathways is crucial for the growth and pathogenicity of bacteria. OmpR was shown to activate csgD expression, resulting in curli production. The CpxR regulator was shown to negatively affect curli gene expression when binding to its recognition site that overlaps the csgD OmpR-binding site. This study was undertaken to clarify how the interplay between the two regulatory proteins, OmpR and CpxR, can affect the transcription of the curli gene in response to variation of the medium osmolarity. Band-shift assays with purified CpxR proteins indicate that CpxR binds to the csgD promoter region at multiple sites that are ideally positioned to explain the csg repression activity of CpxR. To understand the physiological meaning of this in vitro molecular phenomenon, we analyzed the effects of an osmolarity shift on the two-component pathway CpxA/CpxR. We establish here that the Cpx pathway is activated at both transcriptional and posttranscriptional levels in response to a high osmolarity medium and that CpxR represses csgD expression in high-salt-content medium, resulting in low curli production. However, csgD repression in response to high sucrose content is not mediated by CpxR but by the global regulatory protein H-NS. Therefore, multiple systems (EnvZ/OmpR, Cpx, Rcs, and H-NS) appear to be involved in sensing environmental osmolarity, leading to sophisticated regulation of the curli genes.

Authors+Show Affiliations

Unité de Microbiologie et Génétique Composante INSA, Villeurbanne, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15743952

Citation

Jubelin, Gregory, et al. "CpxR/OmpR Interplay Regulates Curli Gene Expression in Response to Osmolarity in Escherichia Coli." Journal of Bacteriology, vol. 187, no. 6, 2005, pp. 2038-49.
Jubelin G, Vianney A, Beloin C, et al. CpxR/OmpR interplay regulates curli gene expression in response to osmolarity in Escherichia coli. J Bacteriol. 2005;187(6):2038-49.
Jubelin, G., Vianney, A., Beloin, C., Ghigo, J. M., Lazzaroni, J. C., Lejeune, P., & Dorel, C. (2005). CpxR/OmpR interplay regulates curli gene expression in response to osmolarity in Escherichia coli. Journal of Bacteriology, 187(6), 2038-49.
Jubelin G, et al. CpxR/OmpR Interplay Regulates Curli Gene Expression in Response to Osmolarity in Escherichia Coli. J Bacteriol. 2005;187(6):2038-49. PubMed PMID: 15743952.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CpxR/OmpR interplay regulates curli gene expression in response to osmolarity in Escherichia coli. AU - Jubelin,Gregory, AU - Vianney,Anne, AU - Beloin,Christophe, AU - Ghigo,Jean-Marc, AU - Lazzaroni,Jean-Claude, AU - Lejeune,Philippe, AU - Dorel,Corinne, PY - 2005/3/4/pubmed PY - 2005/4/9/medline PY - 2005/3/4/entrez SP - 2038 EP - 49 JF - Journal of bacteriology JO - J Bacteriol VL - 187 IS - 6 N2 - Curli fibers could be described as a virulence factor able to confer adherence properties to both abiotic and eukaryotic surfaces. The ability to adapt rapidly to changing environmental conditions through signal transduction pathways is crucial for the growth and pathogenicity of bacteria. OmpR was shown to activate csgD expression, resulting in curli production. The CpxR regulator was shown to negatively affect curli gene expression when binding to its recognition site that overlaps the csgD OmpR-binding site. This study was undertaken to clarify how the interplay between the two regulatory proteins, OmpR and CpxR, can affect the transcription of the curli gene in response to variation of the medium osmolarity. Band-shift assays with purified CpxR proteins indicate that CpxR binds to the csgD promoter region at multiple sites that are ideally positioned to explain the csg repression activity of CpxR. To understand the physiological meaning of this in vitro molecular phenomenon, we analyzed the effects of an osmolarity shift on the two-component pathway CpxA/CpxR. We establish here that the Cpx pathway is activated at both transcriptional and posttranscriptional levels in response to a high osmolarity medium and that CpxR represses csgD expression in high-salt-content medium, resulting in low curli production. However, csgD repression in response to high sucrose content is not mediated by CpxR but by the global regulatory protein H-NS. Therefore, multiple systems (EnvZ/OmpR, Cpx, Rcs, and H-NS) appear to be involved in sensing environmental osmolarity, leading to sophisticated regulation of the curli genes. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/15743952/CpxR/OmpR_interplay_regulates_curli_gene_expression_in_response_to_osmolarity_in_Escherichia_coli_ L2 - https://journals.asm.org/doi/10.1128/JB.187.6.2038-2049.2005?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -