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Antimicrobial-resistant and extraintestinal pathogenic Escherichia coli in retail foods.
J Infect Dis. 2005 Apr 01; 191(7):1040-9.JI

Abstract

BACKGROUND

Extraintestinal Escherichia coli infections are associated with specialized extraintestinal pathogenic E. coli (ExPEC) strains and, increasingly, with antimicrobial resistance. The food supply may disseminate ExPEC and antimicrobial-resistant E. coli.

METHODS

In a prospective survey of 1648 diverse food items from 10 retail markets in the Minneapolis-St. Paul area during 2001-2003, selective cultures and disk-diffusion assays for the isolation and characterization of antimicrobial-resistant E. coli and polymerase chain reaction-based assays and O serotyping to define ExPEC-associated traits were performed.

RESULTS

E. coli contamination exhibited a prevalence gradient from miscellaneous foods (9%), through beef or pork (69%), to poultry (92%; P<.001). Among E. coli-positive samples, similar prevalence gradients were detected for antimicrobial resistance (27%, 85%, and 94% of samples, respectively; P<.001) and ExPEC contamination (4%, 19%, and 46%, respectively; P<.001). By multivariate analysis, beef or pork and poultry from natural-food stores exhibited reduced risks of E. coli contamination and antimicrobial resistance. Indirect evidence suggested on-farm selection of resistance. Four food-source ExPEC isolates (from pea pods, turkey parts, ground pork, and vegetable dip) closely resembled selected human clinical isolates by O antigen and genomic profile.

CONCLUSIONS

Retail foods may be an important vehicle for community-wide dissemination of antimicrobial-resistant E. coli and ExPEC, which may represent a newly recognized group of medically significant foodborne pathogens.

Authors+Show Affiliations

Johnson JR
Mucosal and Vaccine Research Center, Veterans Affairs Medical Center, and Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55417, USA. johns007@umn.edu
Kuskowski MA
No affiliation info available
Smith K
No affiliation info available
O'Bryan TT
No affiliation info available
Tatini S
No affiliation info available

MeSH

AnimalsCattleDrug Resistance, BacterialEscherichia coliEscherichia coli InfectionsFood MicrobiologyFoodborne DiseasesHumansMeatMicrobial Sensitivity TestsO AntigensPolymerase Chain ReactionPoultry ProductsSelection, GeneticSerotypingSwine

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

15747237

Citation

Johnson, James R., et al. "Antimicrobial-resistant and Extraintestinal Pathogenic Escherichia Coli in Retail Foods." The Journal of Infectious Diseases, vol. 191, no. 7, 2005, pp. 1040-9.
Johnson JR, Kuskowski MA, Smith K, et al. Antimicrobial-resistant and extraintestinal pathogenic Escherichia coli in retail foods. J Infect Dis. 2005;191(7):1040-9.
Johnson, J. R., Kuskowski, M. A., Smith, K., O'Bryan, T. T., & Tatini, S. (2005). Antimicrobial-resistant and extraintestinal pathogenic Escherichia coli in retail foods. The Journal of Infectious Diseases, 191(7), 1040-9.
Johnson JR, et al. Antimicrobial-resistant and Extraintestinal Pathogenic Escherichia Coli in Retail Foods. J Infect Dis. 2005 Apr 1;191(7):1040-9. PubMed PMID: 15747237.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial-resistant and extraintestinal pathogenic Escherichia coli in retail foods. AU - Johnson,James R, AU - Kuskowski,Michael A, AU - Smith,Kirk, AU - O'Bryan,Timothy T, AU - Tatini,Sita, Y1 - 2005/03/01/ PY - 2004/06/03/received PY - 2004/09/24/accepted PY - 2005/3/5/pubmed PY - 2005/5/7/medline PY - 2005/3/5/entrez SP - 1040 EP - 9 JF - The Journal of infectious diseases JO - J Infect Dis VL - 191 IS - 7 N2 - BACKGROUND: Extraintestinal Escherichia coli infections are associated with specialized extraintestinal pathogenic E. coli (ExPEC) strains and, increasingly, with antimicrobial resistance. The food supply may disseminate ExPEC and antimicrobial-resistant E. coli. METHODS: In a prospective survey of 1648 diverse food items from 10 retail markets in the Minneapolis-St. Paul area during 2001-2003, selective cultures and disk-diffusion assays for the isolation and characterization of antimicrobial-resistant E. coli and polymerase chain reaction-based assays and O serotyping to define ExPEC-associated traits were performed. RESULTS: E. coli contamination exhibited a prevalence gradient from miscellaneous foods (9%), through beef or pork (69%), to poultry (92%; P<.001). Among E. coli-positive samples, similar prevalence gradients were detected for antimicrobial resistance (27%, 85%, and 94% of samples, respectively; P<.001) and ExPEC contamination (4%, 19%, and 46%, respectively; P<.001). By multivariate analysis, beef or pork and poultry from natural-food stores exhibited reduced risks of E. coli contamination and antimicrobial resistance. Indirect evidence suggested on-farm selection of resistance. Four food-source ExPEC isolates (from pea pods, turkey parts, ground pork, and vegetable dip) closely resembled selected human clinical isolates by O antigen and genomic profile. CONCLUSIONS: Retail foods may be an important vehicle for community-wide dissemination of antimicrobial-resistant E. coli and ExPEC, which may represent a newly recognized group of medically significant foodborne pathogens. SN - 0022-1899 UR - https://www.unboundmedicine.com/medline/citation/15747237/Antimicrobial_resistant_and_extraintestinal_pathogenic_Escherichia_coli_in_retail_foods_ DB - PRIME DP - Unbound Medicine ER -