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Structural assignment of isomeric 2-aminopyridine-derivatized oligosaccharides using negative-ion MSn spectral matching.
Rapid Commun Mass Spectrom. 2005; 19(7):937-46.RC

Abstract

To investigate the possibility of structural assignment based on negative-ion MS2 spectral matching, three isomeric pairs of 2-aminopyridine (PA)-derivatized non-fucosylated, fucosylated, and sialylated oligosaccharides (complex type N-glycans) were analyzed using high-performance liquid chromatography/ion trap mass spectrometry (HPLC/ITMS) with a sonic-spray ionization (SSI) source. In the SSI negative-ion mode the deprotonated molecule [M-2H]2- becomes prominent. Negative-ion MS2 spectra derived from such ions contain many fragment types (B and Y, C and Z, A, and D) and therefore are more informative than the positive-ion MS2 spectra derived from [M+H+Na]2+ ions, which usually consist mainly of B and Y fragment ions. In particular the internal ions (D- and E-type ions) provided useful information about the alpha1-6 branching patterns and the bisecting GlcNAc residue. Spectral matching based on the correlation coefficients between negative-ion MS2 spectra was performed in a manner similar to the positive-ion MS2 spectral matching previously reported. It was demonstrated that negative-ion MS2 spectral matching is as useful and applicable to the structural assignment of relatively large non-fucosylated, fucosylated, and sialylated PA-oligosaccharide isomers as its positive-ion counterpart.

Authors+Show Affiliations

Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 001-0021, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15747328

Citation

Takegawa, Yasuhiro, et al. "Structural Assignment of Isomeric 2-aminopyridine-derivatized Oligosaccharides Using Negative-ion MSn Spectral Matching." Rapid Communications in Mass Spectrometry : RCM, vol. 19, no. 7, 2005, pp. 937-46.
Takegawa Y, Deguchi K, Ito S, et al. Structural assignment of isomeric 2-aminopyridine-derivatized oligosaccharides using negative-ion MSn spectral matching. Rapid Commun Mass Spectrom. 2005;19(7):937-46.
Takegawa, Y., Deguchi, K., Ito, S., Yoshioka, S., Nakagawa, H., & Nishimura, S. (2005). Structural assignment of isomeric 2-aminopyridine-derivatized oligosaccharides using negative-ion MSn spectral matching. Rapid Communications in Mass Spectrometry : RCM, 19(7), 937-46.
Takegawa Y, et al. Structural Assignment of Isomeric 2-aminopyridine-derivatized Oligosaccharides Using Negative-ion MSn Spectral Matching. Rapid Commun Mass Spectrom. 2005;19(7):937-46. PubMed PMID: 15747328.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structural assignment of isomeric 2-aminopyridine-derivatized oligosaccharides using negative-ion MSn spectral matching. AU - Takegawa,Yasuhiro, AU - Deguchi,Kisaburo, AU - Ito,Shinya, AU - Yoshioka,Shinji, AU - Nakagawa,Hiroaki, AU - Nishimura,Shin-Ichiro, PY - 2005/3/5/pubmed PY - 2005/5/12/medline PY - 2005/3/5/entrez SP - 937 EP - 46 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 19 IS - 7 N2 - To investigate the possibility of structural assignment based on negative-ion MS2 spectral matching, three isomeric pairs of 2-aminopyridine (PA)-derivatized non-fucosylated, fucosylated, and sialylated oligosaccharides (complex type N-glycans) were analyzed using high-performance liquid chromatography/ion trap mass spectrometry (HPLC/ITMS) with a sonic-spray ionization (SSI) source. In the SSI negative-ion mode the deprotonated molecule [M-2H]2- becomes prominent. Negative-ion MS2 spectra derived from such ions contain many fragment types (B and Y, C and Z, A, and D) and therefore are more informative than the positive-ion MS2 spectra derived from [M+H+Na]2+ ions, which usually consist mainly of B and Y fragment ions. In particular the internal ions (D- and E-type ions) provided useful information about the alpha1-6 branching patterns and the bisecting GlcNAc residue. Spectral matching based on the correlation coefficients between negative-ion MS2 spectra was performed in a manner similar to the positive-ion MS2 spectral matching previously reported. It was demonstrated that negative-ion MS2 spectral matching is as useful and applicable to the structural assignment of relatively large non-fucosylated, fucosylated, and sialylated PA-oligosaccharide isomers as its positive-ion counterpart. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/15747328/Structural_assignment_of_isomeric_2_aminopyridine_derivatized_oligosaccharides_using_negative_ion_MSn_spectral_matching_ L2 - https://doi.org/10.1002/rcm.1872 DB - PRIME DP - Unbound Medicine ER -