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Capillary electrophoresis-mass spectrometry as a powerful tool in clinical diagnosis and biomarker discovery.
Mass Spectrom Rev. 2005 Nov-Dec; 24(6):959-77.MS

Abstract

Proteome analysis is now emerging as key technology for deciphering biological processes and the discovery of biomarkers for diseases from tissues and body fluids. The complexity and wide dynamic range of protein expression poses a formidable challenge to both peptide separation technologies and mass spectrometry (MS). Here we review the efforts that have been undertaken to date, focussing on capillary electrophoresis coupled to mass spectrometry (CE-MS). We discuss CE-MS from an application point of view evaluating its merits and vices in regard to biomarker discovery and clinical applications. As examples, we present the use of CE-MS for the determination of protein patterns in urine, serum, and other body fluids. Finally, the benefits and limitations of CE-MS for the analysis of proteins in clinical samples are discussed against the background of alternative technologies.

Authors+Show Affiliations

Kolch W
The Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, United Kingdom.
Neusüss C
No affiliation info available
Pelzing M
No affiliation info available
Mischak H
No affiliation info available

MeSH

AnimalsBiomarkersBlood Chemical AnalysisBody FluidsDiagnosis, Computer-AssistedElectrophoresis, CapillaryGene Expression ProfilingHumansMass SpectrometryUrinalysis

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

15747373

Citation

Kolch, Walter, et al. "Capillary Electrophoresis-mass Spectrometry as a Powerful Tool in Clinical Diagnosis and Biomarker Discovery." Mass Spectrometry Reviews, vol. 24, no. 6, 2005, pp. 959-77.
Kolch W, Neusüss C, Pelzing M, et al. Capillary electrophoresis-mass spectrometry as a powerful tool in clinical diagnosis and biomarker discovery. Mass Spectrom Rev. 2005;24(6):959-77.
Kolch, W., Neusüss, C., Pelzing, M., & Mischak, H. (2005). Capillary electrophoresis-mass spectrometry as a powerful tool in clinical diagnosis and biomarker discovery. Mass Spectrometry Reviews, 24(6), 959-77.
Kolch W, et al. Capillary Electrophoresis-mass Spectrometry as a Powerful Tool in Clinical Diagnosis and Biomarker Discovery. Mass Spectrom Rev. 2005 Nov-Dec;24(6):959-77. PubMed PMID: 15747373.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Capillary electrophoresis-mass spectrometry as a powerful tool in clinical diagnosis and biomarker discovery. AU - Kolch,Walter, AU - Neusüss,Christian, AU - Pelzing,Matthias, AU - Mischak,Harald, PY - 2005/3/5/pubmed PY - 2005/12/13/medline PY - 2005/3/5/entrez SP - 959 EP - 77 JF - Mass spectrometry reviews JO - Mass Spectrom Rev VL - 24 IS - 6 N2 - Proteome analysis is now emerging as key technology for deciphering biological processes and the discovery of biomarkers for diseases from tissues and body fluids. The complexity and wide dynamic range of protein expression poses a formidable challenge to both peptide separation technologies and mass spectrometry (MS). Here we review the efforts that have been undertaken to date, focussing on capillary electrophoresis coupled to mass spectrometry (CE-MS). We discuss CE-MS from an application point of view evaluating its merits and vices in regard to biomarker discovery and clinical applications. As examples, we present the use of CE-MS for the determination of protein patterns in urine, serum, and other body fluids. Finally, the benefits and limitations of CE-MS for the analysis of proteins in clinical samples are discussed against the background of alternative technologies. SN - 0277-7037 UR - https://www.unboundmedicine.com/medline/citation/15747373/Capillary_electrophoresis_mass_spectrometry_as_a_powerful_tool_in_clinical_diagnosis_and_biomarker_discovery_ DB - PRIME DP - Unbound Medicine ER -