Tags

Type your tag names separated by a space and hit enter

Endogenous aromatization of testosterone results in growth stimulation of the human MCF-7 breast cancer cell line.
J Steroid Biochem Mol Biol. 2005 Jan; 93(1):25-34.JS

Abstract

Estrogens produced within breast tumors may play a pivotal role in growth stimulation of the breast cancer cells. However, it is elusive whether the epithelial breast cancer cells themselves synthesize estrogens, or whether the surrounding tumor stromal cells synthesize and supply the cancer cells with estrogen. The aromatase enzyme catalyzes the estrogen production, aromatizing circulating androgens into estrogens. The aim of this study was to investigate aromatase expression and function in a model system of human breast cancer, using the estrogen responsive human MCF-7 breast cancer cell line. Cells were cultured in a low estrogen milieu and treated with estrogens, aromatizable androgens or non-aromatizable androgens. Cell proliferation, expression of estrogen-regulated proteins and aromatase activity were investigated. The MCF-7 cell line was observed to express sufficient aromatase enzyme activity in order to aromatize the androgen testosterone, resulting in a significant cell growth stimulation. The testosterone-mediated growth effect was completely inhibited by the aromatase inhibitors letrozole and 4-hydroxy-androstenedione. Expression studies of estrogen-regulated proteins confirmed that testosterone was aromatized to estrogen in the MCF-7 cells. Thus, the results indicate that epithelial breast cancer cells possess the ability to aromatize circulating androgens to estrogens.

Authors+Show Affiliations

Department of Tumor Endocrinology, Institute of Cancer Biology, Danish Cancer Society, Strandboulevarden 49, DK-2100 Copenhagen, Denmark.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15748829

Citation

Sonne-Hansen, K, and A E. Lykkesfeldt. "Endogenous Aromatization of Testosterone Results in Growth Stimulation of the Human MCF-7 Breast Cancer Cell Line." The Journal of Steroid Biochemistry and Molecular Biology, vol. 93, no. 1, 2005, pp. 25-34.
Sonne-Hansen K, Lykkesfeldt AE. Endogenous aromatization of testosterone results in growth stimulation of the human MCF-7 breast cancer cell line. J Steroid Biochem Mol Biol. 2005;93(1):25-34.
Sonne-Hansen, K., & Lykkesfeldt, A. E. (2005). Endogenous aromatization of testosterone results in growth stimulation of the human MCF-7 breast cancer cell line. The Journal of Steroid Biochemistry and Molecular Biology, 93(1), 25-34.
Sonne-Hansen K, Lykkesfeldt AE. Endogenous Aromatization of Testosterone Results in Growth Stimulation of the Human MCF-7 Breast Cancer Cell Line. J Steroid Biochem Mol Biol. 2005;93(1):25-34. PubMed PMID: 15748829.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Endogenous aromatization of testosterone results in growth stimulation of the human MCF-7 breast cancer cell line. AU - Sonne-Hansen,K, AU - Lykkesfeldt,A E, Y1 - 2005/01/27/ PY - 2004/09/01/received PY - 2004/11/23/accepted PY - 2005/3/8/pubmed PY - 2005/5/10/medline PY - 2005/3/8/entrez SP - 25 EP - 34 JF - The Journal of steroid biochemistry and molecular biology JO - J. Steroid Biochem. Mol. Biol. VL - 93 IS - 1 N2 - Estrogens produced within breast tumors may play a pivotal role in growth stimulation of the breast cancer cells. However, it is elusive whether the epithelial breast cancer cells themselves synthesize estrogens, or whether the surrounding tumor stromal cells synthesize and supply the cancer cells with estrogen. The aromatase enzyme catalyzes the estrogen production, aromatizing circulating androgens into estrogens. The aim of this study was to investigate aromatase expression and function in a model system of human breast cancer, using the estrogen responsive human MCF-7 breast cancer cell line. Cells were cultured in a low estrogen milieu and treated with estrogens, aromatizable androgens or non-aromatizable androgens. Cell proliferation, expression of estrogen-regulated proteins and aromatase activity were investigated. The MCF-7 cell line was observed to express sufficient aromatase enzyme activity in order to aromatize the androgen testosterone, resulting in a significant cell growth stimulation. The testosterone-mediated growth effect was completely inhibited by the aromatase inhibitors letrozole and 4-hydroxy-androstenedione. Expression studies of estrogen-regulated proteins confirmed that testosterone was aromatized to estrogen in the MCF-7 cells. Thus, the results indicate that epithelial breast cancer cells possess the ability to aromatize circulating androgens to estrogens. SN - 0960-0760 UR - https://www.unboundmedicine.com/medline/citation/15748829/Endogenous_aromatization_of_testosterone_results_in_growth_stimulation_of_the_human_MCF_7_breast_cancer_cell_line_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-0760(04)00409-1 DB - PRIME DP - Unbound Medicine ER -