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150 kDa glycoprotein isolated from Solanum nigrum Linne enhances activities of detoxicant enzymes and lowers plasmic cholesterol in mouse.
Pharmacol Res. 2005 May; 51(5):399-408.PR

Abstract

This study was carried out to investigate the modulation of detoxicant enzyme activity and plasma lipidemic levels by 150 kDa glycoprotein isolated from Solanum nigrum Linne (SNL), which has been used as a hepatoprotective and anticancer agent in folk medicine. Our results in this study showed that SNL glycoprotein has a band with 150 kDa on the 10% sodium dodecyl sulfate polyacrylamide gel, and that it has a strong scavenging activity against lipid peroxyl radicals. We also evaluated the lipidemic levels of SNL glycoprotein, based on lipoproteins and activities of detoxicant enzymes in treatment with Triton WR-1339 or corn oil in vivo. When mice were treated with either Triton WR-1339 or corn oil in the absence of SNL glycoprotein, the number of plasma lipoproteins [triglyceride (TG), total cholesterol (TC) and low density lipoprotein (LDL)] increased. However, when the mice were treated with either Triton WR-1339 or corn oil in the presence of SNL glycoprotein, the plasma lipoprotein levels (TG, TC and LDL) were significantly reduced. Similar results of SNL glycoprotein treatment were also produced in the activities of detoxicant enzymes. Namely, the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were remarkably increased after treatment with SNL glycoprotein. In addition, the activity of hepatic 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase was reduced by SNL glycoprotein in cholestyramine-treated mice. For example, we found that it inhibits the activity of cholestyramine-induced hepatic HMG-CoA reductase at 40 microg head body weight g(-1) SNL glycoprotein. Collectively, these results pointed out that SNL glycoprotein can enhance the activities of detoxicant enzymes and bring about the inhibition of HMG-CoA reductase activity in vivo. Therefore, we speculate that SNL glycoprotein can be used as a cholesterol-lowering agent even at low concentrations.

Authors+Show Affiliations

#521, Molecular Biochemistry Laboratory and Biodefensive Substances Group, Institute of Biotechnology, Chonnam National University, 500-757 Kwangju, 300 Yongbong-Dong, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15749454

Citation

Lee, Sei-Jung, et al. "150 kDa Glycoprotein Isolated From Solanum Nigrum Linne Enhances Activities of Detoxicant Enzymes and Lowers Plasmic Cholesterol in Mouse." Pharmacological Research, vol. 51, no. 5, 2005, pp. 399-408.
Lee SJ, Ko JH, Lim K, et al. 150 kDa glycoprotein isolated from Solanum nigrum Linne enhances activities of detoxicant enzymes and lowers plasmic cholesterol in mouse. Pharmacol Res. 2005;51(5):399-408.
Lee, S. J., Ko, J. H., Lim, K., & Lim, K. T. (2005). 150 kDa glycoprotein isolated from Solanum nigrum Linne enhances activities of detoxicant enzymes and lowers plasmic cholesterol in mouse. Pharmacological Research, 51(5), 399-408.
Lee SJ, et al. 150 kDa Glycoprotein Isolated From Solanum Nigrum Linne Enhances Activities of Detoxicant Enzymes and Lowers Plasmic Cholesterol in Mouse. Pharmacol Res. 2005;51(5):399-408. PubMed PMID: 15749454.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 150 kDa glycoprotein isolated from Solanum nigrum Linne enhances activities of detoxicant enzymes and lowers plasmic cholesterol in mouse. AU - Lee,Sei-Jung, AU - Ko,Jeong-Hyeon, AU - Lim,Kwang, AU - Lim,Kye-Taek, PY - 2004/11/12/accepted PY - 2005/3/8/pubmed PY - 2005/6/30/medline PY - 2005/3/8/entrez SP - 399 EP - 408 JF - Pharmacological research JO - Pharmacol Res VL - 51 IS - 5 N2 - This study was carried out to investigate the modulation of detoxicant enzyme activity and plasma lipidemic levels by 150 kDa glycoprotein isolated from Solanum nigrum Linne (SNL), which has been used as a hepatoprotective and anticancer agent in folk medicine. Our results in this study showed that SNL glycoprotein has a band with 150 kDa on the 10% sodium dodecyl sulfate polyacrylamide gel, and that it has a strong scavenging activity against lipid peroxyl radicals. We also evaluated the lipidemic levels of SNL glycoprotein, based on lipoproteins and activities of detoxicant enzymes in treatment with Triton WR-1339 or corn oil in vivo. When mice were treated with either Triton WR-1339 or corn oil in the absence of SNL glycoprotein, the number of plasma lipoproteins [triglyceride (TG), total cholesterol (TC) and low density lipoprotein (LDL)] increased. However, when the mice were treated with either Triton WR-1339 or corn oil in the presence of SNL glycoprotein, the plasma lipoprotein levels (TG, TC and LDL) were significantly reduced. Similar results of SNL glycoprotein treatment were also produced in the activities of detoxicant enzymes. Namely, the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were remarkably increased after treatment with SNL glycoprotein. In addition, the activity of hepatic 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase was reduced by SNL glycoprotein in cholestyramine-treated mice. For example, we found that it inhibits the activity of cholestyramine-induced hepatic HMG-CoA reductase at 40 microg head body weight g(-1) SNL glycoprotein. Collectively, these results pointed out that SNL glycoprotein can enhance the activities of detoxicant enzymes and bring about the inhibition of HMG-CoA reductase activity in vivo. Therefore, we speculate that SNL glycoprotein can be used as a cholesterol-lowering agent even at low concentrations. SN - 1043-6618 UR - https://www.unboundmedicine.com/medline/citation/15749454/150_kDa_glycoprotein_isolated_from_Solanum_nigrum_Linne_enhances_activities_of_detoxicant_enzymes_and_lowers_plasmic_cholesterol_in_mouse_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1043-6618(04)00276-2 DB - PRIME DP - Unbound Medicine ER -