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Tissue-specific cadmium and metallothionein levels in rainbow trout chronically acclimated to waterborne or dietary cadmium.
Arch Environ Contam Toxicol. 2005 Apr; 48(3):381-90.AE

Abstract

Rainbow trout were exposed to a sublethal concentration of waterborne Cd (0 or 3 microg/L) or dietary Cd (0 or 500 mg/kg dry wt) for 30 days to induce acclimation, and tissue Cd and metallothionein (MT) levels were examined. The greatest Cd concentrations were observed in the kidney followed by the gills and liver of the fish exposed to Cd via water, but in the gut tissues followed by the kidney, liver, and gills for dietary-exposed fish, reflecting a variation depending on the route of Cd exposure. Some MT was found in the nonacclimated naïve fish with no experience of elevated Cd exposure, and these background MT levels were quite high in the posterior intestine (480 microg/g), cecae (257 microg/g), and liver (248 microg/g) relative to other tissues (7-50 microg/g). With exposure to both waterborne and dietary Cd, MT levels rose significantly in all observed tissues. The increases relative to the control levels of MT in naïve fish were in the order: kidney (5.4 times) > gills (4.6) > liver (1.3) for the waterborne exposure group, and in the order kidney (19.3 times) >> cecae and posterior intestine (approximately 6.5 times) > liver and stomach (approximately 5 times) > midintestine (4.3 times) > gills (2.1 times) for the dietary exposure group. At 24 hours after an acute gastrointestinal dose of Cd (276 microg/kg) infused into the stomach of dietary exposure groups, large increases of total Cd but not MT levels were found in the gut tissues of nonacclimated fish; in the Cd-acclimated fish, the posterior intestine was greatly affected with decreases in Cd (71%), Zn (33%), Cu (70%) and MT (46%) levels, suggesting an enhanced sloughing of tissue materials after infusion. Exposure to Cd did not cause any notable decrease of Zn or Cu in any tissue, except that found in the posterior intestine. However, a molar analysis indicated that although Cd levels remained less than MT binding capacity in both waterborne and dietary exposure groups, the total metal levels (Cd + Zn + Cu) greatly exceeded MT binding capacity in all tissues of Cd-exposed fish, suggesting a potential competition of Cd with other metals for binding sites on MT and non-MT proteins in the tissues.

Authors+Show Affiliations

Chowdhury MJ
Department of Biology, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4K1, Canada. chowdhury.jasim@epa.gov
Baldisserotto B
No affiliation info available
Wood CM
No affiliation info available

MeSH

Adaptation, PhysiologicalAnimalsCadmiumCopperDietEnvironmental MonitoringGastrointestinal TractGillsKidneyLiverMetallothioneinOncorhynchus mykissWater Pollutants, ChemicalZinc

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15750771

Citation

Chowdhury, M J., et al. "Tissue-specific Cadmium and Metallothionein Levels in Rainbow Trout Chronically Acclimated to Waterborne or Dietary Cadmium." Archives of Environmental Contamination and Toxicology, vol. 48, no. 3, 2005, pp. 381-90.
Chowdhury MJ, Baldisserotto B, Wood CM. Tissue-specific cadmium and metallothionein levels in rainbow trout chronically acclimated to waterborne or dietary cadmium. Arch Environ Contam Toxicol. 2005;48(3):381-90.
Chowdhury, M. J., Baldisserotto, B., & Wood, C. M. (2005). Tissue-specific cadmium and metallothionein levels in rainbow trout chronically acclimated to waterborne or dietary cadmium. Archives of Environmental Contamination and Toxicology, 48(3), 381-90.
Chowdhury MJ, Baldisserotto B, Wood CM. Tissue-specific Cadmium and Metallothionein Levels in Rainbow Trout Chronically Acclimated to Waterborne or Dietary Cadmium. Arch Environ Contam Toxicol. 2005;48(3):381-90. PubMed PMID: 15750771.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tissue-specific cadmium and metallothionein levels in rainbow trout chronically acclimated to waterborne or dietary cadmium. AU - Chowdhury,M J, AU - Baldisserotto,B, AU - Wood,C M, Y1 - 2005/02/25/ PY - 2004/03/22/received PY - 2004/09/04/accepted PY - 2005/3/8/pubmed PY - 2005/7/27/medline PY - 2005/3/8/entrez SP - 381 EP - 90 JF - Archives of environmental contamination and toxicology JO - Arch Environ Contam Toxicol VL - 48 IS - 3 N2 - Rainbow trout were exposed to a sublethal concentration of waterborne Cd (0 or 3 microg/L) or dietary Cd (0 or 500 mg/kg dry wt) for 30 days to induce acclimation, and tissue Cd and metallothionein (MT) levels were examined. The greatest Cd concentrations were observed in the kidney followed by the gills and liver of the fish exposed to Cd via water, but in the gut tissues followed by the kidney, liver, and gills for dietary-exposed fish, reflecting a variation depending on the route of Cd exposure. Some MT was found in the nonacclimated naïve fish with no experience of elevated Cd exposure, and these background MT levels were quite high in the posterior intestine (480 microg/g), cecae (257 microg/g), and liver (248 microg/g) relative to other tissues (7-50 microg/g). With exposure to both waterborne and dietary Cd, MT levels rose significantly in all observed tissues. The increases relative to the control levels of MT in naïve fish were in the order: kidney (5.4 times) > gills (4.6) > liver (1.3) for the waterborne exposure group, and in the order kidney (19.3 times) >> cecae and posterior intestine (approximately 6.5 times) > liver and stomach (approximately 5 times) > midintestine (4.3 times) > gills (2.1 times) for the dietary exposure group. At 24 hours after an acute gastrointestinal dose of Cd (276 microg/kg) infused into the stomach of dietary exposure groups, large increases of total Cd but not MT levels were found in the gut tissues of nonacclimated fish; in the Cd-acclimated fish, the posterior intestine was greatly affected with decreases in Cd (71%), Zn (33%), Cu (70%) and MT (46%) levels, suggesting an enhanced sloughing of tissue materials after infusion. Exposure to Cd did not cause any notable decrease of Zn or Cu in any tissue, except that found in the posterior intestine. However, a molar analysis indicated that although Cd levels remained less than MT binding capacity in both waterborne and dietary exposure groups, the total metal levels (Cd + Zn + Cu) greatly exceeded MT binding capacity in all tissues of Cd-exposed fish, suggesting a potential competition of Cd with other metals for binding sites on MT and non-MT proteins in the tissues. SN - 0090-4341 UR - https://www.unboundmedicine.com/medline/citation/15750771/Tissue_specific_cadmium_and_metallothionein_levels_in_rainbow_trout_chronically_acclimated_to_waterborne_or_dietary_cadmium_ DB - PRIME DP - Unbound Medicine ER -