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Increased anandamide induced relaxation in mesenteric arteries of cirrhotic rats: role of cannabinoid and vanilloid receptors.
Gut. 2005 Apr; 54(4):522-7.Gut

Abstract

BACKGROUND AND AIMS

Anandamide is an endocannabinoid that evokes hypotension by interaction with peripheral cannabinoid CB1 receptors and with the perivascular transient receptor potential vanilloid type 1 protein (TRPV1). As anandamide has been implicated in the vasodilated state in advanced cirrhosis, the study investigated whether the mesenteric bed from cirrhotic rats has an altered and selective vasodilator response to anandamide.

METHODS

We assessed vascular sensitivity to anandamide, mRNA and protein expression of cannabinoid CB1 receptor and TRPV1 receptor, and the topographical distribution of cannabinoid CB1 receptors in resistance mesenteric arteries of cirrhotic and control rats.

RESULTS

Mesenteric vessels of cirrhotic animals displayed greater sensitivity to anandamide than control vessels. This vasodilator response was reverted by CB1 or TRPV1 receptor blockade, but not after endothelium denudation or nitric oxide inhibition. Anandamide had no effect on distal femoral arteries. CB1 and TRPV1 receptor protein was higher in cirrhotic than in control vessels. Neither CB1 mRNA nor protein was detected in femoral arteries. Immunochemistry showed that CB1 receptors were mainly in the adventitia and in the endothelial monolayer, with higher expression observed in vessels of cirrhotic rats than in controls.

CONCLUSIONS

These results indicate that anandamide is a selective splanchnic vasodilator in cirrhosis which predominantly acts via interaction with two different types of receptors, CB1 and TRPV1 receptors, which are mainly located in perivascular sensory nerve terminals of the mesenteric resistance arteries of these animals.

Authors+Show Affiliations

Laboratorio Hormonal, Hospital Clinic Universitari, Villarroel 170, Barcelona 08036, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15753538

Citation

Domenicali, M, et al. "Increased Anandamide Induced Relaxation in Mesenteric Arteries of Cirrhotic Rats: Role of Cannabinoid and Vanilloid Receptors." Gut, vol. 54, no. 4, 2005, pp. 522-7.
Domenicali M, Ros J, Fernández-Varo G, et al. Increased anandamide induced relaxation in mesenteric arteries of cirrhotic rats: role of cannabinoid and vanilloid receptors. Gut. 2005;54(4):522-7.
Domenicali, M., Ros, J., Fernández-Varo, G., Cejudo-Martín, P., Crespo, M., Morales-Ruiz, M., Briones, A. M., Campistol, J. M., Arroyo, V., Vila, E., Rodés, J., & Jiménez, W. (2005). Increased anandamide induced relaxation in mesenteric arteries of cirrhotic rats: role of cannabinoid and vanilloid receptors. Gut, 54(4), 522-7.
Domenicali M, et al. Increased Anandamide Induced Relaxation in Mesenteric Arteries of Cirrhotic Rats: Role of Cannabinoid and Vanilloid Receptors. Gut. 2005;54(4):522-7. PubMed PMID: 15753538.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased anandamide induced relaxation in mesenteric arteries of cirrhotic rats: role of cannabinoid and vanilloid receptors. AU - Domenicali,M, AU - Ros,J, AU - Fernández-Varo,G, AU - Cejudo-Martín,P, AU - Crespo,M, AU - Morales-Ruiz,M, AU - Briones,A M, AU - Campistol,J-M, AU - Arroyo,V, AU - Vila,E, AU - Rodés,J, AU - Jiménez,W, PY - 2005/3/9/pubmed PY - 2005/4/12/medline PY - 2005/3/9/entrez SP - 522 EP - 7 JF - Gut JO - Gut VL - 54 IS - 4 N2 - BACKGROUND AND AIMS: Anandamide is an endocannabinoid that evokes hypotension by interaction with peripheral cannabinoid CB1 receptors and with the perivascular transient receptor potential vanilloid type 1 protein (TRPV1). As anandamide has been implicated in the vasodilated state in advanced cirrhosis, the study investigated whether the mesenteric bed from cirrhotic rats has an altered and selective vasodilator response to anandamide. METHODS: We assessed vascular sensitivity to anandamide, mRNA and protein expression of cannabinoid CB1 receptor and TRPV1 receptor, and the topographical distribution of cannabinoid CB1 receptors in resistance mesenteric arteries of cirrhotic and control rats. RESULTS: Mesenteric vessels of cirrhotic animals displayed greater sensitivity to anandamide than control vessels. This vasodilator response was reverted by CB1 or TRPV1 receptor blockade, but not after endothelium denudation or nitric oxide inhibition. Anandamide had no effect on distal femoral arteries. CB1 and TRPV1 receptor protein was higher in cirrhotic than in control vessels. Neither CB1 mRNA nor protein was detected in femoral arteries. Immunochemistry showed that CB1 receptors were mainly in the adventitia and in the endothelial monolayer, with higher expression observed in vessels of cirrhotic rats than in controls. CONCLUSIONS: These results indicate that anandamide is a selective splanchnic vasodilator in cirrhosis which predominantly acts via interaction with two different types of receptors, CB1 and TRPV1 receptors, which are mainly located in perivascular sensory nerve terminals of the mesenteric resistance arteries of these animals. SN - 0017-5749 UR - https://www.unboundmedicine.com/medline/citation/15753538/Increased_anandamide_induced_relaxation_in_mesenteric_arteries_of_cirrhotic_rats:_role_of_cannabinoid_and_vanilloid_receptors_ L2 - https://gut.bmj.com/lookup/pmidlookup?view=long&pmid=15753538 DB - PRIME DP - Unbound Medicine ER -