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Molecular cytogenetics and tandem repeat sequence evolution in the allopolyploid Nicotiana rustica compared with diploid progenitors N. paniculata and N. undulata.
Cytogenet Genome Res 2005; 109(1-3):298-309CG

Abstract

Nicotiana rustica (2n = 4x = 48) is a natural allotetraploid composed of P and U genomes which are closely related to genomes of diploid species N. paniculata and N. undulata. Genomic in situ hybridization (GISH) also confirms that the diploid parents, or close relatives, are the ancestors of N. rustica. In order to study genetic interactions between ancestral genomes in the allotetraploid, we isolated three families of repetitive sequences, two from N. paniculata (NPAMBE and NPAMBO) and one from N. undulata (NUNSSP). Southern blot hybridization revealed that the sequences are digested with a range of restriction enzymes into regular ladder patterns indicating a tandem arrangement of high copy repeats possessing monomeric units of about 180 bp. The three-tandem sequences belong to a larger Nicotiana tandem repeat family called here the HRS-60 family. Members of this family are found in all Nicotiana species studied. Fluorescence in situ hybridization (FISH) analysis localized the satellite repeats to subtelomeric regions of most chromosomes of N. paniculata and N. undulata. The pattern of sequence distribution on the P- and U-genomes of N. rustica was similar to the putative parents N. paniculata and N. undulata respectively. However, NPAMBO repeats appear to be reduced and rearranged in N. rustica that may suggest evolution within the P genome. GISH and FISH with the tandem repeat probes failed to reveal intergenomic translocations as might be predicted from the nucleocytoplasmic interaction hypothesis.

Authors+Show Affiliations

School of Biological Sciences, Queen Mary University of London, London, United Kingdom.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15753590

Citation

Lim, K Y., et al. "Molecular Cytogenetics and Tandem Repeat Sequence Evolution in the Allopolyploid Nicotiana Rustica Compared With Diploid Progenitors N. Paniculata and N. Undulata." Cytogenetic and Genome Research, vol. 109, no. 1-3, 2005, pp. 298-309.
Lim KY, Matyasek R, Kovarik A, et al. Molecular cytogenetics and tandem repeat sequence evolution in the allopolyploid Nicotiana rustica compared with diploid progenitors N. paniculata and N. undulata. Cytogenet Genome Res. 2005;109(1-3):298-309.
Lim, K. Y., Matyasek, R., Kovarik, A., Fulnecek, J., & Leitch, A. R. (2005). Molecular cytogenetics and tandem repeat sequence evolution in the allopolyploid Nicotiana rustica compared with diploid progenitors N. paniculata and N. undulata. Cytogenetic and Genome Research, 109(1-3), pp. 298-309.
Lim KY, et al. Molecular Cytogenetics and Tandem Repeat Sequence Evolution in the Allopolyploid Nicotiana Rustica Compared With Diploid Progenitors N. Paniculata and N. Undulata. Cytogenet Genome Res. 2005;109(1-3):298-309. PubMed PMID: 15753590.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cytogenetics and tandem repeat sequence evolution in the allopolyploid Nicotiana rustica compared with diploid progenitors N. paniculata and N. undulata. AU - Lim,K Y, AU - Matyasek,R, AU - Kovarik,A, AU - Fulnecek,J, AU - Leitch,A R, PY - 2003/10/17/received PY - 2004/03/26/accepted PY - 2005/3/9/pubmed PY - 2005/10/28/medline PY - 2005/3/9/entrez SP - 298 EP - 309 JF - Cytogenetic and genome research JO - Cytogenet. Genome Res. VL - 109 IS - 1-3 N2 - Nicotiana rustica (2n = 4x = 48) is a natural allotetraploid composed of P and U genomes which are closely related to genomes of diploid species N. paniculata and N. undulata. Genomic in situ hybridization (GISH) also confirms that the diploid parents, or close relatives, are the ancestors of N. rustica. In order to study genetic interactions between ancestral genomes in the allotetraploid, we isolated three families of repetitive sequences, two from N. paniculata (NPAMBE and NPAMBO) and one from N. undulata (NUNSSP). Southern blot hybridization revealed that the sequences are digested with a range of restriction enzymes into regular ladder patterns indicating a tandem arrangement of high copy repeats possessing monomeric units of about 180 bp. The three-tandem sequences belong to a larger Nicotiana tandem repeat family called here the HRS-60 family. Members of this family are found in all Nicotiana species studied. Fluorescence in situ hybridization (FISH) analysis localized the satellite repeats to subtelomeric regions of most chromosomes of N. paniculata and N. undulata. The pattern of sequence distribution on the P- and U-genomes of N. rustica was similar to the putative parents N. paniculata and N. undulata respectively. However, NPAMBO repeats appear to be reduced and rearranged in N. rustica that may suggest evolution within the P genome. GISH and FISH with the tandem repeat probes failed to reveal intergenomic translocations as might be predicted from the nucleocytoplasmic interaction hypothesis. SN - 1424-859X UR - https://www.unboundmedicine.com/medline/citation/15753590/Molecular_cytogenetics_and_tandem_repeat_sequence_evolution_in_the_allopolyploid_Nicotiana_rustica_compared_with_diploid_progenitors_N__paniculata_and_N__undulata_ L2 - https://www.karger.com?DOI=10.1159/000082413 DB - PRIME DP - Unbound Medicine ER -