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Detection of alien chromatin introgression from Thinopyrum into wheat using S genomic DNA as a probe--a landmark approach for Thinopyrum genome research.
Cytogenet Genome Res. 2005; 109(1-3):350-9.CG

Abstract

The introduction of alien genetic variation from the genus Thinopyrum through chromosome engineering into wheat is a valuable and proven technique for wheat improvement. A number of economically important traits have been transferred into wheat as single genes, chromosome arms or entire chromosomes. Successful transfers can be greatly assisted by the precise identification of alien chromatin in the recipient progenies. Chromosome identification and characterization are useful for genetic manipulation and transfer in wheat breeding following chromosome engineering. Genomic in situ hybridization (GISH) using an S genomic DNA probe from the diploid species Pseudoroegneria has proven to be a powerful diagnostic cytogenetic tool for monitoring the transfer of many promising agronomic traits from Thinopyrum. This specific S genomic probe not only allows the direct determination of the chromosome composition in wheat-Thinopyrum hybrids, but also can separate the Th. intermedium chromosomes into the J, J(S) and S genomes. The J(S) genome, which consists of a modified J genome chromosome distinguished by S genomic sequences of Pseudoroegneria near the centromere and telomere, carries many disease and mite resistance genes. Utilization of this S genomic probe leads to a better understanding of genomic affinities between Thinopyrum and wheat, and provides a molecular cytogenetic marker for monitoring the transfer of alien Thinopyrum agronomic traits into wheat recipient lines.

Authors+Show Affiliations

Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, Alta, Canada. chenqi@agr.gc.ca

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

15753596

Citation

Chen, Q. "Detection of Alien Chromatin Introgression From Thinopyrum Into Wheat Using S Genomic DNA as a Probe--a Landmark Approach for Thinopyrum Genome Research." Cytogenetic and Genome Research, vol. 109, no. 1-3, 2005, pp. 350-9.
Chen Q. Detection of alien chromatin introgression from Thinopyrum into wheat using S genomic DNA as a probe--a landmark approach for Thinopyrum genome research. Cytogenet Genome Res. 2005;109(1-3):350-9.
Chen, Q. (2005). Detection of alien chromatin introgression from Thinopyrum into wheat using S genomic DNA as a probe--a landmark approach for Thinopyrum genome research. Cytogenetic and Genome Research, 109(1-3), 350-9.
Chen Q. Detection of Alien Chromatin Introgression From Thinopyrum Into Wheat Using S Genomic DNA as a Probe--a Landmark Approach for Thinopyrum Genome Research. Cytogenet Genome Res. 2005;109(1-3):350-9. PubMed PMID: 15753596.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of alien chromatin introgression from Thinopyrum into wheat using S genomic DNA as a probe--a landmark approach for Thinopyrum genome research. A1 - Chen,Q, PY - 2003/10/20/received PY - 2004/02/05/accepted PY - 2005/3/9/pubmed PY - 2005/10/28/medline PY - 2005/3/9/entrez SP - 350 EP - 9 JF - Cytogenetic and genome research JO - Cytogenet Genome Res VL - 109 IS - 1-3 N2 - The introduction of alien genetic variation from the genus Thinopyrum through chromosome engineering into wheat is a valuable and proven technique for wheat improvement. A number of economically important traits have been transferred into wheat as single genes, chromosome arms or entire chromosomes. Successful transfers can be greatly assisted by the precise identification of alien chromatin in the recipient progenies. Chromosome identification and characterization are useful for genetic manipulation and transfer in wheat breeding following chromosome engineering. Genomic in situ hybridization (GISH) using an S genomic DNA probe from the diploid species Pseudoroegneria has proven to be a powerful diagnostic cytogenetic tool for monitoring the transfer of many promising agronomic traits from Thinopyrum. This specific S genomic probe not only allows the direct determination of the chromosome composition in wheat-Thinopyrum hybrids, but also can separate the Th. intermedium chromosomes into the J, J(S) and S genomes. The J(S) genome, which consists of a modified J genome chromosome distinguished by S genomic sequences of Pseudoroegneria near the centromere and telomere, carries many disease and mite resistance genes. Utilization of this S genomic probe leads to a better understanding of genomic affinities between Thinopyrum and wheat, and provides a molecular cytogenetic marker for monitoring the transfer of alien Thinopyrum agronomic traits into wheat recipient lines. SN - 1424-859X UR - https://www.unboundmedicine.com/medline/citation/15753596/Detection_of_alien_chromatin_introgression_from_Thinopyrum_into_wheat_using_S_genomic_DNA_as_a_probe__a_landmark_approach_for_Thinopyrum_genome_research_ L2 - https://www.karger.com?DOI=10.1159/000082419 DB - PRIME DP - Unbound Medicine ER -