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Cytogenetics of Triticum x Dasypyrum hybrids and derived lines.
Cytogenet Genome Res. 2005; 109(1-3):385-92.CG

Abstract

Genomic in situ hybridization was used to study Triticum x Dasypyrum wide hybrids and derived lines. A cytogenetic investigation was carried out in progenies of (i) amphiploids derived from T. turgidum var. durum (T. durum; 2n = 14; genomes AABB) x D. villosum (2n = 14; genome VV), (ii) three-parental hybrids (T. durum x D. villosum) x T. aestivum (2n = 42, genomes A'A'B'B'D'D'), and (iii) T. aestivum aneuploid lines carrying D. villosum chromosomes or chromatin. The amphiploids derived from T. durum x D. villosum showed a stable chromosomal constitution, made up of 14 V chromosomes, 14 chromosomes carrying the wheat A genome and 14 chromosomes carrying the B genome. High karyological instability was observed in the progenies of three-parental hybrids ([T. durum x D. villosum] x T. aestivum). Plants having the expected 14 A chromosomes, 14 B chromosomes, 7 D chromosomes, and 7 V chromosomes were rather rare (4.5%). Many progeny plants (45.5%) had the hexaploid wheat genome with 42 chromosomes and lacked any detectable D. villosum chromatin. Other plants (50%) had 14 A chromosomes and 14 B chromosomes, plus variable numbers of D and V chromosomes, the former being better retained than the latter in most cases. Some T. aestivum lines carrying D. villosum chromosomes or chromatin, as the result of addition, substitution, or recombination events or even a combination of these karyological events, were found to be stable. Other lines were unstable, and these lines carried 1V, 3V, or 5V chromosomes or their portions. Substitution or recombination events where 1V chromosomes were involved could concern the homeologous counterparts in both the A and B and D genomes of wheat. No line could be recovered where the shorter arm of 3V chromosomes was present. Changes in the morphology and banding pattern of V chromosomes were observed in hybrids that did not carry the entire D. villosum complement. By comparing the results of our cytogenetic analyses with certain phenotypic characteristics of the lines studied, genes for discrete traits could be assigned to specific V chromosomes or V chromosome arms. From the frequency of V chromosomes that were involved in chromatin exchanges with or substituted for one of their homeologous counterparts in the A, B, and D wheat genomes, it was inferred that D. villosum belongs to the same phyletic lineage as T. urartu (donor of the A genome of wheat) and Aegilops speltoides (B genome), and that Ae. squarrosa (D genome) diverged earlier from D. villosum.

Authors+Show Affiliations

Dipartimento di Biologia Cellulare e Molecolare, Sezione di Citologia e Genetica, Università degli Studi di Perugia, Perugia, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15753601

Citation

Minelli, S, et al. "Cytogenetics of Triticum X Dasypyrum Hybrids and Derived Lines." Cytogenetic and Genome Research, vol. 109, no. 1-3, 2005, pp. 385-92.
Minelli S, Ceccarelli M, Mariani M, et al. Cytogenetics of Triticum x Dasypyrum hybrids and derived lines. Cytogenet Genome Res. 2005;109(1-3):385-92.
Minelli, S., Ceccarelli, M., Mariani, M., De Pace, C., & Cionini, P. G. (2005). Cytogenetics of Triticum x Dasypyrum hybrids and derived lines. Cytogenetic and Genome Research, 109(1-3), 385-92.
Minelli S, et al. Cytogenetics of Triticum X Dasypyrum Hybrids and Derived Lines. Cytogenet Genome Res. 2005;109(1-3):385-92. PubMed PMID: 15753601.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytogenetics of Triticum x Dasypyrum hybrids and derived lines. AU - Minelli,S, AU - Ceccarelli,M, AU - Mariani,M, AU - De Pace,C, AU - Cionini,P G, PY - 2003/11/10/received PY - 2004/05/10/accepted PY - 2005/3/9/pubmed PY - 2005/10/28/medline PY - 2005/3/9/entrez SP - 385 EP - 92 JF - Cytogenetic and genome research JO - Cytogenet Genome Res VL - 109 IS - 1-3 N2 - Genomic in situ hybridization was used to study Triticum x Dasypyrum wide hybrids and derived lines. A cytogenetic investigation was carried out in progenies of (i) amphiploids derived from T. turgidum var. durum (T. durum; 2n = 14; genomes AABB) x D. villosum (2n = 14; genome VV), (ii) three-parental hybrids (T. durum x D. villosum) x T. aestivum (2n = 42, genomes A'A'B'B'D'D'), and (iii) T. aestivum aneuploid lines carrying D. villosum chromosomes or chromatin. The amphiploids derived from T. durum x D. villosum showed a stable chromosomal constitution, made up of 14 V chromosomes, 14 chromosomes carrying the wheat A genome and 14 chromosomes carrying the B genome. High karyological instability was observed in the progenies of three-parental hybrids ([T. durum x D. villosum] x T. aestivum). Plants having the expected 14 A chromosomes, 14 B chromosomes, 7 D chromosomes, and 7 V chromosomes were rather rare (4.5%). Many progeny plants (45.5%) had the hexaploid wheat genome with 42 chromosomes and lacked any detectable D. villosum chromatin. Other plants (50%) had 14 A chromosomes and 14 B chromosomes, plus variable numbers of D and V chromosomes, the former being better retained than the latter in most cases. Some T. aestivum lines carrying D. villosum chromosomes or chromatin, as the result of addition, substitution, or recombination events or even a combination of these karyological events, were found to be stable. Other lines were unstable, and these lines carried 1V, 3V, or 5V chromosomes or their portions. Substitution or recombination events where 1V chromosomes were involved could concern the homeologous counterparts in both the A and B and D genomes of wheat. No line could be recovered where the shorter arm of 3V chromosomes was present. Changes in the morphology and banding pattern of V chromosomes were observed in hybrids that did not carry the entire D. villosum complement. By comparing the results of our cytogenetic analyses with certain phenotypic characteristics of the lines studied, genes for discrete traits could be assigned to specific V chromosomes or V chromosome arms. From the frequency of V chromosomes that were involved in chromatin exchanges with or substituted for one of their homeologous counterparts in the A, B, and D wheat genomes, it was inferred that D. villosum belongs to the same phyletic lineage as T. urartu (donor of the A genome of wheat) and Aegilops speltoides (B genome), and that Ae. squarrosa (D genome) diverged earlier from D. villosum. SN - 1424-859X UR - https://www.unboundmedicine.com/medline/citation/15753601/Cytogenetics_of_Triticum_x_Dasypyrum_hybrids_and_derived_lines_ L2 - https://www.karger.com?DOI=10.1159/000082424 DB - PRIME DP - Unbound Medicine ER -