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Identification of EhICP1, a chagasin-like cysteine protease inhibitor of Entamoeba histolytica.
FEBS Lett. 2005 Mar 14; 579(7):1573-8.FL

Abstract

Based on the Entamoeba histolytica genome project (www.sanger.ac.uk/Project/E_histolytical/) we have identified a cysteine protease inhibitor, EhICP1 (amoebiasin 1), with significant homology to chagasin. Recombinant EhICP1 inhibited the protease activity of papain and that of a trophozoite lysate with Ki's in the picomolar range. By immunocytology, we localized the endogenous approximately 13 kDa EhICP1 in a finely dotted subcellular distribution discrete from the vesicles containing the amoebic cysteine protease, EhCP1 (amoebapain). In an overlay assay, we observed binding of recombinant EhICP1 to EhCP1. As a heptapeptide (GNPTTGF) corresponding to the second conserved chagasin motif inhibited the protease activity of both papain (K) 1.5 microM) and trophozoite extract (Ki in sub-mM range), it may be a candidate for the rational development of anti-amoebiasis drugs.

Authors+Show Affiliations

Riekenberg S
Department of Biology/Chemistry, University of Osnabrueck, 49069 Osnabrueck, Germany.
Witjes B
No affiliation info available
Sarić M
No affiliation info available
Bruchhaus I
No affiliation info available
Scholze H
No affiliation info available

MeSH

Amino Acid SequenceAnimalsCysteine EndopeptidasesCysteine Proteinase InhibitorsEntamoeba histolyticaMolecular Sequence DataPapainProtein Structure, SecondaryRecombinant ProteinsSequence Alignment

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15757643

Citation

Riekenberg, S, et al. "Identification of EhICP1, a Chagasin-like Cysteine Protease Inhibitor of Entamoeba Histolytica." FEBS Letters, vol. 579, no. 7, 2005, pp. 1573-8.
Riekenberg S, Witjes B, Sarić M, et al. Identification of EhICP1, a chagasin-like cysteine protease inhibitor of Entamoeba histolytica. FEBS Lett. 2005;579(7):1573-8.
Riekenberg, S., Witjes, B., Sarić, M., Bruchhaus, I., & Scholze, H. (2005). Identification of EhICP1, a chagasin-like cysteine protease inhibitor of Entamoeba histolytica. FEBS Letters, 579(7), 1573-8.
Riekenberg S, et al. Identification of EhICP1, a Chagasin-like Cysteine Protease Inhibitor of Entamoeba Histolytica. FEBS Lett. 2005 Mar 14;579(7):1573-8. PubMed PMID: 15757643.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of EhICP1, a chagasin-like cysteine protease inhibitor of Entamoeba histolytica. AU - Riekenberg,S, AU - Witjes,B, AU - Sarić,M, AU - Bruchhaus,I, AU - Scholze,H, PY - 2004/12/15/received PY - 2005/01/13/revised PY - 2005/01/27/accepted PY - 2005/3/11/pubmed PY - 2005/4/26/medline PY - 2005/3/11/entrez SP - 1573 EP - 8 JF - FEBS letters JO - FEBS Lett VL - 579 IS - 7 N2 - Based on the Entamoeba histolytica genome project (www.sanger.ac.uk/Project/E_histolytical/) we have identified a cysteine protease inhibitor, EhICP1 (amoebiasin 1), with significant homology to chagasin. Recombinant EhICP1 inhibited the protease activity of papain and that of a trophozoite lysate with Ki's in the picomolar range. By immunocytology, we localized the endogenous approximately 13 kDa EhICP1 in a finely dotted subcellular distribution discrete from the vesicles containing the amoebic cysteine protease, EhCP1 (amoebapain). In an overlay assay, we observed binding of recombinant EhICP1 to EhCP1. As a heptapeptide (GNPTTGF) corresponding to the second conserved chagasin motif inhibited the protease activity of both papain (K) 1.5 microM) and trophozoite extract (Ki in sub-mM range), it may be a candidate for the rational development of anti-amoebiasis drugs. SN - 0014-5793 UR - https://www.unboundmedicine.com/medline/citation/15757643/Identification_of_EhICP1_a_chagasin_like_cysteine_protease_inhibitor_of_Entamoeba_histolytica_ DB - PRIME DP - Unbound Medicine ER -