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Colorado potato beetle larvae on potato plants expressing a locust proteinase inhibitor.
Commun Agric Appl Biol Sci. 2004; 69(3):281-7.CA

Abstract

The natural defence system of plants often involves inhibitors of digestive enzymes of their pests. Modem and environmental-friendly methods try to increase this plant resistance by expressing heterologous protease inhibitors in crops. Here we report the effects of expressing a gene from desert locust (Schistocerca gregaria) encoding two serine protease inhibitors in potato on Colorado potato beetle (Leptinotarsa decemlineata) larvae. The gene encoding both peptides on a single chain was used for Agrobacterium-mediated transformation of potato plants. The presence of the active inhibitor protein in the leaves was verified. The feeding bioassays in the laboratory showed that despite the low level of the peptide in leaves, CPB larvae on transgenic plants have grown slightly but significantly more slowly than those on control potato plants. The results support the notion that expression of multifunctional proteinase inhibitors of insect origin in plants might be a good strategy to improve insect resistance.

Authors+Show Affiliations

Kutas J
Entomological Laboratory, Plant Protection Institute of Georgikon, Faculty of Agricultural Sciences of Veszprém University, H-8360 Keszthely, Deák F. Str. 57., Hungary.
Kondrák M
No affiliation info available
Szenthe B
No affiliation info available
Patthy A
No affiliation info available
Bánfalvi Z
No affiliation info available
Nádasy M
No affiliation info available
Gráf L
No affiliation info available
Asbóth B
No affiliation info available

MeSH

AnimalsCloning, MolecularColeopteraGrasshoppersLarvaPest Control, BiologicalPlants, Genetically ModifiedSerine Proteinase InhibitorsSolanum tuberosum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15759425

Citation

Kutas, J, et al. "Colorado Potato Beetle Larvae On Potato Plants Expressing a Locust Proteinase Inhibitor." Communications in Agricultural and Applied Biological Sciences, vol. 69, no. 3, 2004, pp. 281-7.
Kutas J, Kondrák M, Szenthe B, et al. Colorado potato beetle larvae on potato plants expressing a locust proteinase inhibitor. Commun Agric Appl Biol Sci. 2004;69(3):281-7.
Kutas, J., Kondrák, M., Szenthe, B., Patthy, A., Bánfalvi, Z., Nádasy, M., Gráf, L., & Asbóth, B. (2004). Colorado potato beetle larvae on potato plants expressing a locust proteinase inhibitor. Communications in Agricultural and Applied Biological Sciences, 69(3), 281-7.
Kutas J, et al. Colorado Potato Beetle Larvae On Potato Plants Expressing a Locust Proteinase Inhibitor. Commun Agric Appl Biol Sci. 2004;69(3):281-7. PubMed PMID: 15759425.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Colorado potato beetle larvae on potato plants expressing a locust proteinase inhibitor. AU - Kutas,J, AU - Kondrák,M, AU - Szenthe,B, AU - Patthy,A, AU - Bánfalvi,Z, AU - Nádasy,M, AU - Gráf,L, AU - Asbóth,B, PY - 2005/3/12/pubmed PY - 2005/4/14/medline PY - 2005/3/12/entrez SP - 281 EP - 7 JF - Communications in agricultural and applied biological sciences JO - Commun Agric Appl Biol Sci VL - 69 IS - 3 N2 - The natural defence system of plants often involves inhibitors of digestive enzymes of their pests. Modem and environmental-friendly methods try to increase this plant resistance by expressing heterologous protease inhibitors in crops. Here we report the effects of expressing a gene from desert locust (Schistocerca gregaria) encoding two serine protease inhibitors in potato on Colorado potato beetle (Leptinotarsa decemlineata) larvae. The gene encoding both peptides on a single chain was used for Agrobacterium-mediated transformation of potato plants. The presence of the active inhibitor protein in the leaves was verified. The feeding bioassays in the laboratory showed that despite the low level of the peptide in leaves, CPB larvae on transgenic plants have grown slightly but significantly more slowly than those on control potato plants. The results support the notion that expression of multifunctional proteinase inhibitors of insect origin in plants might be a good strategy to improve insect resistance. SN - 1379-1176 UR - https://www.unboundmedicine.com/medline/citation/15759425/Colorado_potato_beetle_larvae_on_potato_plants_expressing_a_locust_proteinase_inhibitor_ DB - PRIME DP - Unbound Medicine ER -