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Determination of six kavalactones in dietary supplements and selected functional foods containing Piper methysticum by isocratic liquid chromatography with internal standard.
J AOAC Int. 2005 Jan-Feb; 88(1):16-25.JA

Abstract

Kava (Piper methysticum) dietary products have been sold worldwide for treatment of nervous anxiety, tension, and restlessness. Recent reports showed potential association of kava usage and liver injuries. This study was conducted to develop simple and reliable methodologies for the extraction and determination of 6 major kavalactones: (+)-methysticin, (+)-dihydromethysticin, (+)-kavain, (+)-dihydrokavain, yangonin, and desmethoxyyangonin. Ultrasonic extraction techniques and isocratic reversed-phase liquid chromatography (LC) were optimized for different types of samples, including capsules containing kava root extract or root powder, raw root material, tea bags, and snack bar. A suitable internal standard, 5,7-dihydroxyflavone, was used for LC calibration. Kavalactones were completely separated in 30 min using a Luna C18-2 column at 60 degrees C with an isocratic mobile phase consisting of 2-propanol-acetonitrile-water-acetic acid (16 + 16 + 68 + 0.1, v/v/v/v). Within-laboratory, intraday, and interday method variation (% relative standard deviation) for most samples extracted by methanol or methanol-water mixture were <5%. Lower levels of kavalactone contents and higher variations were observed for tea bags from water extraction or infusion as compared to methanol extraction. Labeling information of tea bags based on methanol extraction could be misleading to consumers. Analytical recoveries of snack bar fortified at 10 and 20 microg/g were >84% with RSD values <8%. Methods developed in this study offer a simple and reproducible means for analysis of kavalactones in various matrixes of dietary products.

Authors+Show Affiliations

U.S. Food and Drug Administration, National Center for Toxicological Research, Division of Chemistry, HFT-230, 3900 NCTR Rd, Jefferson, AR 72079, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15759721

Citation

Hu, Lihong, et al. "Determination of Six Kavalactones in Dietary Supplements and Selected Functional Foods Containing Piper Methysticum By Isocratic Liquid Chromatography With Internal Standard." Journal of AOAC International, vol. 88, no. 1, 2005, pp. 16-25.
Hu L, Jhoo JW, Ang CY, et al. Determination of six kavalactones in dietary supplements and selected functional foods containing Piper methysticum by isocratic liquid chromatography with internal standard. J AOAC Int. 2005;88(1):16-25.
Hu, L., Jhoo, J. W., Ang, C. Y., Dinovi, M., & Mattia, A. (2005). Determination of six kavalactones in dietary supplements and selected functional foods containing Piper methysticum by isocratic liquid chromatography with internal standard. Journal of AOAC International, 88(1), 16-25.
Hu L, et al. Determination of Six Kavalactones in Dietary Supplements and Selected Functional Foods Containing Piper Methysticum By Isocratic Liquid Chromatography With Internal Standard. J AOAC Int. 2005 Jan-Feb;88(1):16-25. PubMed PMID: 15759721.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of six kavalactones in dietary supplements and selected functional foods containing Piper methysticum by isocratic liquid chromatography with internal standard. AU - Hu,Lihong, AU - Jhoo,Jin-Woo, AU - Ang,Catharina Y W, AU - Dinovi,Michael, AU - Mattia,Antonia, PY - 2005/3/12/pubmed PY - 2005/7/29/medline PY - 2005/3/12/entrez SP - 16 EP - 25 JF - Journal of AOAC International JO - J AOAC Int VL - 88 IS - 1 N2 - Kava (Piper methysticum) dietary products have been sold worldwide for treatment of nervous anxiety, tension, and restlessness. Recent reports showed potential association of kava usage and liver injuries. This study was conducted to develop simple and reliable methodologies for the extraction and determination of 6 major kavalactones: (+)-methysticin, (+)-dihydromethysticin, (+)-kavain, (+)-dihydrokavain, yangonin, and desmethoxyyangonin. Ultrasonic extraction techniques and isocratic reversed-phase liquid chromatography (LC) were optimized for different types of samples, including capsules containing kava root extract or root powder, raw root material, tea bags, and snack bar. A suitable internal standard, 5,7-dihydroxyflavone, was used for LC calibration. Kavalactones were completely separated in 30 min using a Luna C18-2 column at 60 degrees C with an isocratic mobile phase consisting of 2-propanol-acetonitrile-water-acetic acid (16 + 16 + 68 + 0.1, v/v/v/v). Within-laboratory, intraday, and interday method variation (% relative standard deviation) for most samples extracted by methanol or methanol-water mixture were <5%. Lower levels of kavalactone contents and higher variations were observed for tea bags from water extraction or infusion as compared to methanol extraction. Labeling information of tea bags based on methanol extraction could be misleading to consumers. Analytical recoveries of snack bar fortified at 10 and 20 microg/g were >84% with RSD values <8%. Methods developed in this study offer a simple and reproducible means for analysis of kavalactones in various matrixes of dietary products. SN - 1060-3271 UR - https://www.unboundmedicine.com/medline/citation/15759721/Determination_of_six_kavalactones_in_dietary_supplements_and_selected_functional_foods_containing_Piper_methysticum_by_isocratic_liquid_chromatography_with_internal_standard_ L2 - https://medlineplus.gov/dietarysupplements.html DB - PRIME DP - Unbound Medicine ER -