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Preferential elimination of repeated DNA sequences from the paternal, Nicotiana tomentosiformis genome donor of a synthetic, allotetraploid tobacco.
New Phytol 2005; 166(1):291-303NP

Abstract

Nicotiana tabacum (tobacco, 2n = 4x = 48) is a natural allotetraploid combining two ancestral genomes closely related to modern Nicotiana sylvestris and Nicotiana tomentosiformis. Here we examine the immediate consequences of allopolyploidy on genome evolution using 20 S4-generation plants derived from a single synthetic, S0 plant made by Burk in 1973 (Th37). Using molecular and cytogenetic methods we analysed 14 middle and highly repetitive sequences that together total approximately 4% of the genome. Two repeats related to endogenous geminiviruses (GRD5) and pararetroviruses (NtoEPRV), and two classes of satellite repeats (NTRS, A1/A2) were partially or completely eliminated at variable frequency (25-60%). These sequences are all from the N. tomentosiformis parent. Genomic in situ hybridization revealed additivity in chromosome numbers in two plants (2n = 48), while a third was aneuploid for an N. tomentosiformis-origin chromosome (2n = 49). Two plants had homozygous translocations between chromosomes of the S- and T-genomes. * The data demonstrate that genetic changes in synthetic tobacco were fast, targeted to the paternal N. tomentosiformis-donated genome, and some of the changes showed concordance with changes that presumably occurred during evolution of natural tobacco.

Authors+Show Affiliations

Institute of Biophysics, Academy of Sciences of the Czech Republic, CZ-61265 Brno, Czech Republic.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15760371

Citation

Skalická, K, et al. "Preferential Elimination of Repeated DNA Sequences From the Paternal, Nicotiana Tomentosiformis Genome Donor of a Synthetic, Allotetraploid Tobacco." The New Phytologist, vol. 166, no. 1, 2005, pp. 291-303.
Skalická K, Lim KY, Matyasek R, et al. Preferential elimination of repeated DNA sequences from the paternal, Nicotiana tomentosiformis genome donor of a synthetic, allotetraploid tobacco. New Phytol. 2005;166(1):291-303.
Skalická, K., Lim, K. Y., Matyasek, R., Matzke, M., Leitch, A. R., & Kovarik, A. (2005). Preferential elimination of repeated DNA sequences from the paternal, Nicotiana tomentosiformis genome donor of a synthetic, allotetraploid tobacco. The New Phytologist, 166(1), pp. 291-303.
Skalická K, et al. Preferential Elimination of Repeated DNA Sequences From the Paternal, Nicotiana Tomentosiformis Genome Donor of a Synthetic, Allotetraploid Tobacco. New Phytol. 2005;166(1):291-303. PubMed PMID: 15760371.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preferential elimination of repeated DNA sequences from the paternal, Nicotiana tomentosiformis genome donor of a synthetic, allotetraploid tobacco. AU - Skalická,K, AU - Lim,K Y, AU - Matyasek,R, AU - Matzke,M, AU - Leitch,A R, AU - Kovarik,A, PY - 2005/3/12/pubmed PY - 2005/6/23/medline PY - 2005/3/12/entrez SP - 291 EP - 303 JF - The New phytologist JO - New Phytol. VL - 166 IS - 1 N2 - Nicotiana tabacum (tobacco, 2n = 4x = 48) is a natural allotetraploid combining two ancestral genomes closely related to modern Nicotiana sylvestris and Nicotiana tomentosiformis. Here we examine the immediate consequences of allopolyploidy on genome evolution using 20 S4-generation plants derived from a single synthetic, S0 plant made by Burk in 1973 (Th37). Using molecular and cytogenetic methods we analysed 14 middle and highly repetitive sequences that together total approximately 4% of the genome. Two repeats related to endogenous geminiviruses (GRD5) and pararetroviruses (NtoEPRV), and two classes of satellite repeats (NTRS, A1/A2) were partially or completely eliminated at variable frequency (25-60%). These sequences are all from the N. tomentosiformis parent. Genomic in situ hybridization revealed additivity in chromosome numbers in two plants (2n = 48), while a third was aneuploid for an N. tomentosiformis-origin chromosome (2n = 49). Two plants had homozygous translocations between chromosomes of the S- and T-genomes. * The data demonstrate that genetic changes in synthetic tobacco were fast, targeted to the paternal N. tomentosiformis-donated genome, and some of the changes showed concordance with changes that presumably occurred during evolution of natural tobacco. SN - 0028-646X UR - https://www.unboundmedicine.com/medline/citation/15760371/Preferential_elimination_of_repeated_DNA_sequences_from_the_paternal_Nicotiana_tomentosiformis_genome_donor_of_a_synthetic_allotetraploid_tobacco_ L2 - https://doi.org/10.1111/j.1469-8137.2004.01297.x DB - PRIME DP - Unbound Medicine ER -