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Cytokine profile and proteome analysis in bronchoalveolar lavage of patients with sarcoidosis, pulmonary fibrosis associated with systemic sclerosis and idiopathic pulmonary fibrosis.
Proteomics. 2005 Apr; 5(5):1423-30.P

Abstract

The aim of this study was to analyze the type of immune response (Th1, Th2) and protein composition of bronchoalveolar lavage (BAL) of patients with sarcoidosis, pulmonary fibrosis associated with systemic sclerosis (SSc) and idiopathic pulmonary fibrosis (IPF). Flow cytometry analysis of intracellular cytokines revealed different patterns: in IPF and SSc Th2 profiles were predominant, whereas in sarcoidosis Th1 prevailed. The proteomic analysis of BAL fluid (BALF) showed that there were quantitative differences between the three diseases. These were more evident between sarcoidosis and IPF, confirming our previous observations, whereas SSc had an intermediate profile between the two, however with some peculiarities. Comparison of BALF protein maps, constructed with the same quantity of total proteins, enabled us to identify the main profiles of the three diseases: an increase in plasma protein prevalent in sarcoidosis and also present in SSc, though for fewer proteins with respect to IPF and a greater abundance of low molecular weight proteins, mainly locally produced, in IPF. These findings are in line with the different pathogenesis of these diseases: IPF is considered a prevalently fibrotic disorder limited to the lung, with intense local production of functionally different proteins, whereas sarcoidosis and SSc are systemic immunoinflammatory diseases.

Authors+Show Affiliations

Respiratory Diseases Section, Department of Clinical Medicine and Immunological Sciences, University of Siena, Siena, Italy. rottoli@unisi.it <rottoli@unisi.it>No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15761959

Citation

Rottoli, Paola, et al. "Cytokine Profile and Proteome Analysis in Bronchoalveolar Lavage of Patients With Sarcoidosis, Pulmonary Fibrosis Associated With Systemic Sclerosis and Idiopathic Pulmonary Fibrosis." Proteomics, vol. 5, no. 5, 2005, pp. 1423-30.
Rottoli P, Magi B, Perari MG, et al. Cytokine profile and proteome analysis in bronchoalveolar lavage of patients with sarcoidosis, pulmonary fibrosis associated with systemic sclerosis and idiopathic pulmonary fibrosis. Proteomics. 2005;5(5):1423-30.
Rottoli, P., Magi, B., Perari, M. G., Liberatori, S., Nikiforakis, N., Bargagli, E., Cianti, R., Bini, L., & Pallini, V. (2005). Cytokine profile and proteome analysis in bronchoalveolar lavage of patients with sarcoidosis, pulmonary fibrosis associated with systemic sclerosis and idiopathic pulmonary fibrosis. Proteomics, 5(5), 1423-30.
Rottoli P, et al. Cytokine Profile and Proteome Analysis in Bronchoalveolar Lavage of Patients With Sarcoidosis, Pulmonary Fibrosis Associated With Systemic Sclerosis and Idiopathic Pulmonary Fibrosis. Proteomics. 2005;5(5):1423-30. PubMed PMID: 15761959.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytokine profile and proteome analysis in bronchoalveolar lavage of patients with sarcoidosis, pulmonary fibrosis associated with systemic sclerosis and idiopathic pulmonary fibrosis. AU - Rottoli,Paola, AU - Magi,Barbara, AU - Perari,Maria Grazia, AU - Liberatori,Sabrina, AU - Nikiforakis,Nikolaos, AU - Bargagli,Elena, AU - Cianti,Riccardo, AU - Bini,Luca, AU - Pallini,Vitaliano, PY - 2005/3/12/pubmed PY - 2005/10/1/medline PY - 2005/3/12/entrez SP - 1423 EP - 30 JF - Proteomics JO - Proteomics VL - 5 IS - 5 N2 - The aim of this study was to analyze the type of immune response (Th1, Th2) and protein composition of bronchoalveolar lavage (BAL) of patients with sarcoidosis, pulmonary fibrosis associated with systemic sclerosis (SSc) and idiopathic pulmonary fibrosis (IPF). Flow cytometry analysis of intracellular cytokines revealed different patterns: in IPF and SSc Th2 profiles were predominant, whereas in sarcoidosis Th1 prevailed. The proteomic analysis of BAL fluid (BALF) showed that there were quantitative differences between the three diseases. These were more evident between sarcoidosis and IPF, confirming our previous observations, whereas SSc had an intermediate profile between the two, however with some peculiarities. Comparison of BALF protein maps, constructed with the same quantity of total proteins, enabled us to identify the main profiles of the three diseases: an increase in plasma protein prevalent in sarcoidosis and also present in SSc, though for fewer proteins with respect to IPF and a greater abundance of low molecular weight proteins, mainly locally produced, in IPF. These findings are in line with the different pathogenesis of these diseases: IPF is considered a prevalently fibrotic disorder limited to the lung, with intense local production of functionally different proteins, whereas sarcoidosis and SSc are systemic immunoinflammatory diseases. SN - 1615-9853 UR - https://www.unboundmedicine.com/medline/citation/15761959/Cytokine_profile_and_proteome_analysis_in_bronchoalveolar_lavage_of_patients_with_sarcoidosis_pulmonary_fibrosis_associated_with_systemic_sclerosis_and_idiopathic_pulmonary_fibrosis_ L2 - https://doi.org/10.1002/pmic.200301007 DB - PRIME DP - Unbound Medicine ER -