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Mutational pattern of influenza B viruses adapted to high growth replication in embryonated eggs.
Virus Res. 2005 May; 109(2):149-57.VR

Abstract

Improved replication of influenza viruses in embryonated chicken eggs (CE) permits increased vaccine production and availability. We investigated the growth properties of influenza B viruses in relation to specific mutations occurring after serial passage in CE. In serial passage experiments yielding high growth variants of B/Victoria/504/2000, mutations predicted to alter amino acid (AA) composition occurred only near the receptor-binding pocket of the hemagglutinins (HA) and in no other genes. Two B/Victoria/504/2000 high growth variants had the same AA substitutions in HA (R162M and D196Y), but the higher yield variant had a third substitution (G141E), which also altered antigenic characteristics. In a serial passage experiment yielding a high growth variant of B/Hong Kong/330/2001, mutations predicted to alter AA composition occurred only in PB2 and NP in domains predicted to relate to RNP formation and function. Our results indicate that adaptation of influenza B viruses to high-yield replication by serial passage in CE requires few mutations either in internal or external genes. Specific modifications of genes or a combination of genes could be used to optimize or create influenza B viruses for specific growth substrates.

Authors+Show Affiliations

Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, 8800 Rockville Pike, Bethesda, MD 20892, USA. lugovtsev@cber.fda.govNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15763145

Citation

Lugovtsev, Vladimir Y., et al. "Mutational Pattern of Influenza B Viruses Adapted to High Growth Replication in Embryonated Eggs." Virus Research, vol. 109, no. 2, 2005, pp. 149-57.
Lugovtsev VY, Vodeiko GM, Levandowski RA. Mutational pattern of influenza B viruses adapted to high growth replication in embryonated eggs. Virus Res. 2005;109(2):149-57.
Lugovtsev, V. Y., Vodeiko, G. M., & Levandowski, R. A. (2005). Mutational pattern of influenza B viruses adapted to high growth replication in embryonated eggs. Virus Research, 109(2), 149-57.
Lugovtsev VY, Vodeiko GM, Levandowski RA. Mutational Pattern of Influenza B Viruses Adapted to High Growth Replication in Embryonated Eggs. Virus Res. 2005;109(2):149-57. PubMed PMID: 15763145.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mutational pattern of influenza B viruses adapted to high growth replication in embryonated eggs. AU - Lugovtsev,Vladimir Y, AU - Vodeiko,Galina M, AU - Levandowski,Roland A, Y1 - 2004/12/29/ PY - 2004/08/17/received PY - 2004/11/12/revised PY - 2004/11/12/accepted PY - 2005/3/15/pubmed PY - 2005/7/23/medline PY - 2005/3/15/entrez SP - 149 EP - 57 JF - Virus research JO - Virus Res. VL - 109 IS - 2 N2 - Improved replication of influenza viruses in embryonated chicken eggs (CE) permits increased vaccine production and availability. We investigated the growth properties of influenza B viruses in relation to specific mutations occurring after serial passage in CE. In serial passage experiments yielding high growth variants of B/Victoria/504/2000, mutations predicted to alter amino acid (AA) composition occurred only near the receptor-binding pocket of the hemagglutinins (HA) and in no other genes. Two B/Victoria/504/2000 high growth variants had the same AA substitutions in HA (R162M and D196Y), but the higher yield variant had a third substitution (G141E), which also altered antigenic characteristics. In a serial passage experiment yielding a high growth variant of B/Hong Kong/330/2001, mutations predicted to alter AA composition occurred only in PB2 and NP in domains predicted to relate to RNP formation and function. Our results indicate that adaptation of influenza B viruses to high-yield replication by serial passage in CE requires few mutations either in internal or external genes. Specific modifications of genes or a combination of genes could be used to optimize or create influenza B viruses for specific growth substrates. SN - 0168-1702 UR - https://www.unboundmedicine.com/medline/citation/15763145/Mutational_pattern_of_influenza_B_viruses_adapted_to_high_growth_replication_in_embryonated_eggs_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1702(04)00435-6 DB - PRIME DP - Unbound Medicine ER -