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Postnatal changes in the spatial distributions of substance P and neurokinin-1 receptor in the trigeminal subnucleus caudalis of mice.
Brain Res Dev Brain Res. 2005 Mar 22; 155(1):33-41.BR

Abstract

Nociceptive afferent signals from the orofacial area are transmitted to the trigeminal subnucleus caudalis (Vc) through the release of glutamate and/or substance P (SP). Although nociceptive transmission and/or modulating mechanisms are known to develop during the postnatal period, the specific developmental changes in nociception and/or modulation remain unclear. The present study examined postnatal changes in the spatial relationship between SP and its receptor, the NK1 receptor (NK1R), in the mouse Vc by immunohistochemistry and quantitative analysis. The medulla was removed from C57BL/6N mice (1, 2, 4, and 8 weeks of age) after perfusion and fixation, and cut horizontally at a thickness of 40 mum. The relative densities of SP- and NK1R-immunoreactive areas and their changes with age were assessed statistically. One- and 2-week-old mice showed relatively high densities of SP-positive structures in the marginal layer (Mar) and the deep part of the magnocellular layer (Mag). The SP distribution in the superficial Vc remained unchanged, but the density in the deep Mag gradually decreased with age, resulting in a complete loss after postnatal week 4. The NK1R-immunoreactivity exhibited a similar distribution pattern to that of SP, but the pattern remained unchanged during the postnatal period. Double-immunofluorescence staining for SP and NK1R demonstrated only moderate direct contact of SP-positive structures with NK1R in the superficial area. These separate distributions and the postnatal changes in SP and NK1R suggest the possibility of another nociceptive afferent transmission mechanism, that is, volume transmission, in the Vc other than synapse-mediated transmission.

Authors+Show Affiliations

Division of Oral and Maxillofacial Surgery, Department of Oral Health Sciences, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15763273

Citation

Aita, Megumi, et al. "Postnatal Changes in the Spatial Distributions of Substance P and Neurokinin-1 Receptor in the Trigeminal Subnucleus Caudalis of Mice." Brain Research. Developmental Brain Research, vol. 155, no. 1, 2005, pp. 33-41.
Aita M, Seo K, Fujiwara N, et al. Postnatal changes in the spatial distributions of substance P and neurokinin-1 receptor in the trigeminal subnucleus caudalis of mice. Brain Res Dev Brain Res. 2005;155(1):33-41.
Aita, M., Seo, K., Fujiwara, N., Takagi, R., & Maeda, T. (2005). Postnatal changes in the spatial distributions of substance P and neurokinin-1 receptor in the trigeminal subnucleus caudalis of mice. Brain Research. Developmental Brain Research, 155(1), 33-41.
Aita M, et al. Postnatal Changes in the Spatial Distributions of Substance P and Neurokinin-1 Receptor in the Trigeminal Subnucleus Caudalis of Mice. Brain Res Dev Brain Res. 2005 Mar 22;155(1):33-41. PubMed PMID: 15763273.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Postnatal changes in the spatial distributions of substance P and neurokinin-1 receptor in the trigeminal subnucleus caudalis of mice. AU - Aita,Megumi, AU - Seo,Kenji, AU - Fujiwara,Naoshi, AU - Takagi,Ritsuo, AU - Maeda,Takeyasu, PY - 2004/08/09/received PY - 2004/12/01/revised PY - 2004/12/02/accepted PY - 2005/3/15/pubmed PY - 2005/6/24/medline PY - 2005/3/15/entrez SP - 33 EP - 41 JF - Brain research. Developmental brain research JO - Brain Res. Dev. Brain Res. VL - 155 IS - 1 N2 - Nociceptive afferent signals from the orofacial area are transmitted to the trigeminal subnucleus caudalis (Vc) through the release of glutamate and/or substance P (SP). Although nociceptive transmission and/or modulating mechanisms are known to develop during the postnatal period, the specific developmental changes in nociception and/or modulation remain unclear. The present study examined postnatal changes in the spatial relationship between SP and its receptor, the NK1 receptor (NK1R), in the mouse Vc by immunohistochemistry and quantitative analysis. The medulla was removed from C57BL/6N mice (1, 2, 4, and 8 weeks of age) after perfusion and fixation, and cut horizontally at a thickness of 40 mum. The relative densities of SP- and NK1R-immunoreactive areas and their changes with age were assessed statistically. One- and 2-week-old mice showed relatively high densities of SP-positive structures in the marginal layer (Mar) and the deep part of the magnocellular layer (Mag). The SP distribution in the superficial Vc remained unchanged, but the density in the deep Mag gradually decreased with age, resulting in a complete loss after postnatal week 4. The NK1R-immunoreactivity exhibited a similar distribution pattern to that of SP, but the pattern remained unchanged during the postnatal period. Double-immunofluorescence staining for SP and NK1R demonstrated only moderate direct contact of SP-positive structures with NK1R in the superficial area. These separate distributions and the postnatal changes in SP and NK1R suggest the possibility of another nociceptive afferent transmission mechanism, that is, volume transmission, in the Vc other than synapse-mediated transmission. SN - 0165-3806 UR - https://www.unboundmedicine.com/medline/citation/15763273/Postnatal_changes_in_the_spatial_distributions_of_substance_P_and_neurokinin_1_receptor_in_the_trigeminal_subnucleus_caudalis_of_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-3806(04)00376-1 DB - PRIME DP - Unbound Medicine ER -