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Fructose diet-induced skin collagen abnormalities are prevented by lipoic acid.
Exp Diabesity Res. 2004 Oct-Dec; 5(4):237-44.ED

Abstract

Nonenzymatic glycation of proteins, leading to chemical modification and cross-linking are of importance in the pathology of diabetic complications. We studied the effect alpha-lipoic acid (LA) on the content and characteristics of the protein collagen from skin of high-fructose fed rats. The rats were divided into 4 groups of 6 each. Two groups of rats were fed with a high fructose diet (60 g/100 g diet) and administered either LA (35 mg/kg b.w., i.p) (FRU+LA) or 0.2 ml vehicle (saline) (FRU) for 45 days. The other 2 groups were fed with control diet containing starch (60 g/100 g diet) and administered either saline (CON) or lipoic acid (CON+LA). The rats were maintained for 45 days and then sacrificed. Plasma glucose, insulin, fructosamine, protein glycation, and blood glycated hemoglobin (HbA1C) were measured. Collagen was isolated from skin and the physicochemical properties of collagen were studied. Fructose administration caused accumulation of collagen in skin. Extensive cross-linking was evidenced by enhanced glycation and AGE-linked fluorescence. Increased peroxidation and changes in physicochemical properties such as shrinkage temperature, aldehyde content, solubililty pattern, susceptibility to denaturing agents were observed in fructose-fed rats. SDS gel pattern of collagen from these rats showed elevated beta component of type I collagen. These changes were alleviated by the simultaneous administration of LA. Administration of LA to fructose-fed rats had a positive influence on both quantitative and qualitative properties of collagen. The results suggest a mechanism for the ability of LA to delay diabetic complications.

Authors+Show Affiliations

Department of Biochemistry, Faculty of Science, Annamalai University, Annamalai Nagar, India.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15763937

Citation

Thirunavukkarasu, V, et al. "Fructose Diet-induced Skin Collagen Abnormalities Are Prevented By Lipoic Acid." Experimental Diabesity Research, vol. 5, no. 4, 2004, pp. 237-44.
Thirunavukkarasu V, Nandhini AT, Anuradha CV. Fructose diet-induced skin collagen abnormalities are prevented by lipoic acid. Exp Diabesity Res. 2004;5(4):237-44.
Thirunavukkarasu, V., Nandhini, A. T., & Anuradha, C. V. (2004). Fructose diet-induced skin collagen abnormalities are prevented by lipoic acid. Experimental Diabesity Research, 5(4), 237-44.
Thirunavukkarasu V, Nandhini AT, Anuradha CV. Fructose Diet-induced Skin Collagen Abnormalities Are Prevented By Lipoic Acid. Exp Diabesity Res. 2004 Oct-Dec;5(4):237-44. PubMed PMID: 15763937.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fructose diet-induced skin collagen abnormalities are prevented by lipoic acid. AU - Thirunavukkarasu,V, AU - Nandhini,A T Anitha, AU - Anuradha,C V, PY - 2005/3/15/pubmed PY - 2005/4/27/medline PY - 2005/3/15/entrez SP - 237 EP - 44 JF - Experimental diabesity research JO - Exp. Diabesity Res. VL - 5 IS - 4 N2 - Nonenzymatic glycation of proteins, leading to chemical modification and cross-linking are of importance in the pathology of diabetic complications. We studied the effect alpha-lipoic acid (LA) on the content and characteristics of the protein collagen from skin of high-fructose fed rats. The rats were divided into 4 groups of 6 each. Two groups of rats were fed with a high fructose diet (60 g/100 g diet) and administered either LA (35 mg/kg b.w., i.p) (FRU+LA) or 0.2 ml vehicle (saline) (FRU) for 45 days. The other 2 groups were fed with control diet containing starch (60 g/100 g diet) and administered either saline (CON) or lipoic acid (CON+LA). The rats were maintained for 45 days and then sacrificed. Plasma glucose, insulin, fructosamine, protein glycation, and blood glycated hemoglobin (HbA1C) were measured. Collagen was isolated from skin and the physicochemical properties of collagen were studied. Fructose administration caused accumulation of collagen in skin. Extensive cross-linking was evidenced by enhanced glycation and AGE-linked fluorescence. Increased peroxidation and changes in physicochemical properties such as shrinkage temperature, aldehyde content, solubililty pattern, susceptibility to denaturing agents were observed in fructose-fed rats. SDS gel pattern of collagen from these rats showed elevated beta component of type I collagen. These changes were alleviated by the simultaneous administration of LA. Administration of LA to fructose-fed rats had a positive influence on both quantitative and qualitative properties of collagen. The results suggest a mechanism for the ability of LA to delay diabetic complications. SN - 1543-8600 UR - https://www.unboundmedicine.com/medline/citation/15763937/Fructose_diet_induced_skin_collagen_abnormalities_are_prevented_by_lipoic_acid_ L2 - https://dx.doi.org/10.1080/154386090506148 DB - PRIME DP - Unbound Medicine ER -