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Watermelon (Citrullus lanatus) hydroperoxide lyase greatly increases C6 aldehyde formation in transgenic leaves.
J Agric Food Chem. 2005 Mar 23; 53(6):2046-51.JA

Abstract

Fatty acid hydroperoxide lyase (HL) is the key enzyme for the production of the "green note"compounds, leaf aldehyde [(2E)-hexenal] and leaf alcohol [(3Z)-hexenol], in plant tissues. A cDNA encoding HL was cloned from leaves of watermelon (Citrullus lanatus) and expressed in Nicotiana tabacum. The enzyme is 3 times more active with 13-hydroperoxylinolenic acid than with 13-hydroperoxylinoleic acid. The activity against 9-hydroperoxides of polyunsaturated fatty acids is minimal. Enzyme activity of the watermelon HL in the transgenic leaves was approximately 50 times higher than endogenous HL activity in the wild-type N. tabacum plants. When compared with Arabidopsis HL also expressed in N. tabacum, the highest HL activity is 10 times higher in watermelon HL overexpressing leaves than in Arabidopsis HL overexpressers.

Authors+Show Affiliations

Fukushige H
Department of Agronomy, University of Kentucky, 1405 Veterans Drive, Lexington, Kentucky 40546-0312, USA.
Hildebrand DF
No affiliation info available

MeSH

Aldehyde-LyasesAldehydesAmino Acid SequenceBase SequenceCitrullusCloning, MolecularCytochrome P-450 Enzyme SystemGene ExpressionMolecular Sequence DataPhylogenyPlant LeavesPlants, Genetically ModifiedReverse Transcriptase Polymerase Chain ReactionSequence AlignmentTobacco

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

15769134

Citation

Fukushige, Hirotada, and David F. Hildebrand. "Watermelon (Citrullus Lanatus) Hydroperoxide Lyase Greatly Increases C6 Aldehyde Formation in Transgenic Leaves." Journal of Agricultural and Food Chemistry, vol. 53, no. 6, 2005, pp. 2046-51.
Fukushige H, Hildebrand DF. Watermelon (Citrullus lanatus) hydroperoxide lyase greatly increases C6 aldehyde formation in transgenic leaves. J Agric Food Chem. 2005;53(6):2046-51.
Fukushige, H., & Hildebrand, D. F. (2005). Watermelon (Citrullus lanatus) hydroperoxide lyase greatly increases C6 aldehyde formation in transgenic leaves. Journal of Agricultural and Food Chemistry, 53(6), 2046-51.
Fukushige H, Hildebrand DF. Watermelon (Citrullus Lanatus) Hydroperoxide Lyase Greatly Increases C6 Aldehyde Formation in Transgenic Leaves. J Agric Food Chem. 2005 Mar 23;53(6):2046-51. PubMed PMID: 15769134.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Watermelon (Citrullus lanatus) hydroperoxide lyase greatly increases C6 aldehyde formation in transgenic leaves. AU - Fukushige,Hirotada, AU - Hildebrand,David F, PY - 2005/3/17/pubmed PY - 2005/4/29/medline PY - 2005/3/17/entrez SP - 2046 EP - 51 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 53 IS - 6 N2 - Fatty acid hydroperoxide lyase (HL) is the key enzyme for the production of the "green note"compounds, leaf aldehyde [(2E)-hexenal] and leaf alcohol [(3Z)-hexenol], in plant tissues. A cDNA encoding HL was cloned from leaves of watermelon (Citrullus lanatus) and expressed in Nicotiana tabacum. The enzyme is 3 times more active with 13-hydroperoxylinolenic acid than with 13-hydroperoxylinoleic acid. The activity against 9-hydroperoxides of polyunsaturated fatty acids is minimal. Enzyme activity of the watermelon HL in the transgenic leaves was approximately 50 times higher than endogenous HL activity in the wild-type N. tabacum plants. When compared with Arabidopsis HL also expressed in N. tabacum, the highest HL activity is 10 times higher in watermelon HL overexpressing leaves than in Arabidopsis HL overexpressers. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/15769134/Watermelon__Citrullus_lanatus__hydroperoxide_lyase_greatly_increases_C6_aldehyde_formation_in_transgenic_leaves_ DB - PRIME DP - Unbound Medicine ER -