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[Association between high-risk human papillomavirus DNA load and cervical intraepithelial lesion].

Abstract

OBJECTIVE

To determine the association between viral load of high risk type human papillomavirus (HR-HPV) and stage of cervical intraepithelial neoplasia (CIN) lesion.

METHODS

Cervical exfoliated cells were collected from 1997 women aged 35-45 in a cross-sectional screening study. HPV DNA was detected by hybrid capture 2 (HC2) system, and viral load was measured by the ratios of relative light units compared to standard positive control (RLU/PC). Log10RLU/PC were categorized into four groups: negative (< 0), low viral load (0 - 1.12), medium viral load (1.13 - 2.23), and high viral load (2.24 - 3.37). Cervical lesions were diagnosed by biopsies as normal, CIN 1, CIN 2-3, and squamous cervical cancer (SCC). Association between HR-HPV and CINs were evaluated by unconditional multinomial logistic regression.

RESULTS

100% (12/12) SCC, 97.3% (72/74) of CIN 2-3, 58.3% (74/127) of CIN 1, and 11.5% (205/1784) of normal women were positive for HPV DNA. The median log10RLUs for the positive women with SCC, CIN 2-3, CIN 1 and in normal women were 2.60, 2.32, 2.18 and 1.18 respectively. The odds ratio (OR) between low viral load of HPV DNA and CIN 1 was 3.8 (1.9 - 7.3) while between high viral load and CIN 2-3 was OR=865.9 (200.1 - 3738.0) which showed that higher viral load could increase the risk of cervical lesions (P <0.001).

CONCLUSION

Both cervical cancer and CINs were highly influenced by HR-HPV viral load.

Authors+Show Affiliations

Department of Epidemiology, Cancer Institute/Hospital, Chinese Academy of Medical Sciences, Beijing 100021, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

15769315

Citation

Zhao, Fang-hui, et al. "[Association Between High-risk Human Papillomavirus DNA Load and Cervical Intraepithelial Lesion]." Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi, vol. 25, no. 11, 2004, pp. 921-4.
Zhao FH, Ma JF, Qiao YL, et al. [Association between high-risk human papillomavirus DNA load and cervical intraepithelial lesion]. Zhonghua Liu Xing Bing Xue Za Zhi. 2004;25(11):921-4.
Zhao, F. H., Ma, J. F., Qiao, Y. L., Rong, S. D., Li, L., & Zhang, W. H. (2004). [Association between high-risk human papillomavirus DNA load and cervical intraepithelial lesion]. Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi, 25(11), pp. 921-4.
Zhao FH, et al. [Association Between High-risk Human Papillomavirus DNA Load and Cervical Intraepithelial Lesion]. Zhonghua Liu Xing Bing Xue Za Zhi. 2004;25(11):921-4. PubMed PMID: 15769315.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Association between high-risk human papillomavirus DNA load and cervical intraepithelial lesion]. AU - Zhao,Fang-hui, AU - Ma,Jun-fei, AU - Qiao,You-lin, AU - Rong,Shou-de, AU - Li,Ling, AU - Zhang,Wen-hua, PY - 2005/3/17/pubmed PY - 2006/3/11/medline PY - 2005/3/17/entrez SP - 921 EP - 4 JF - Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi JO - Zhonghua Liu Xing Bing Xue Za Zhi VL - 25 IS - 11 N2 - OBJECTIVE: To determine the association between viral load of high risk type human papillomavirus (HR-HPV) and stage of cervical intraepithelial neoplasia (CIN) lesion. METHODS: Cervical exfoliated cells were collected from 1997 women aged 35-45 in a cross-sectional screening study. HPV DNA was detected by hybrid capture 2 (HC2) system, and viral load was measured by the ratios of relative light units compared to standard positive control (RLU/PC). Log10RLU/PC were categorized into four groups: negative (< 0), low viral load (0 - 1.12), medium viral load (1.13 - 2.23), and high viral load (2.24 - 3.37). Cervical lesions were diagnosed by biopsies as normal, CIN 1, CIN 2-3, and squamous cervical cancer (SCC). Association between HR-HPV and CINs were evaluated by unconditional multinomial logistic regression. RESULTS: 100% (12/12) SCC, 97.3% (72/74) of CIN 2-3, 58.3% (74/127) of CIN 1, and 11.5% (205/1784) of normal women were positive for HPV DNA. The median log10RLUs for the positive women with SCC, CIN 2-3, CIN 1 and in normal women were 2.60, 2.32, 2.18 and 1.18 respectively. The odds ratio (OR) between low viral load of HPV DNA and CIN 1 was 3.8 (1.9 - 7.3) while between high viral load and CIN 2-3 was OR=865.9 (200.1 - 3738.0) which showed that higher viral load could increase the risk of cervical lesions (P <0.001). CONCLUSION: Both cervical cancer and CINs were highly influenced by HR-HPV viral load. SN - 0254-6450 UR - https://www.unboundmedicine.com/medline/citation/15769315/[Association_between_high_risk_human_papillomavirus_DNA_load_and_cervical_intraepithelial_lesion]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=0254-6450&amp;year=2004&amp;vol=25&amp;issue=11&amp;fpage=921 DB - PRIME DP - Unbound Medicine ER -