Tags

Type your tag names separated by a space and hit enter

Parathyroid hormone decreases renal vitamin D receptor expression in vivo.
Proc Natl Acad Sci U S A 2005; 102(13):4724-8PN

Abstract

The vitamin D receptor (VDR) is a nuclear transcription factor responsible for mediating the biological activities of 1,25-dihydroxyvitamin D(3) [1,25(OH)(2)D(3)]. Renal and parathyroid gland VDR content is an important factor in calcium homeostasis, vitamin D metabolism, and the treatment of secondary hyperparathyroidism and renal osteodystrophy. In these tissues, VDR expression is highly regulated by the calcium and vitamin D status. Although 1,25(OH)(2)D(3) up-regulates VDR expression, hypocalcemia and vitamin D deficiency result in drastically reduced expression of the receptor. The generation of 25-hydroxyvitamin D(3)-1alpha-hydroxylase-null mice, which are incapable of endogenously producing 1,25(OH)(2)D(3), has allowed us to investigate the influence of parathyroid hormone (PTH) on VDR expression independent of PTH-mediated increases in 1,25(OH)(2)D(3). Administration of human PTH (1-34) (110 microg/kg per day) for 48 h reduced renal VDR levels from 515 to 435 fmol/mg protein (15%, P < 0.03) in wild-type mice. In the 25-hydroxyvitamin D(3)-1alpha-hydroxylase-null mice, PTH administration strongly reduced renal VDR levels, from 555 to 394 fmol/mg protein (29%, P < 0.001). These results demonstrate that PTH is a potent down-regulator of VDR expression in vivo.

Authors+Show Affiliations

Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15769857

Citation

Healy, Kevin D., et al. "Parathyroid Hormone Decreases Renal Vitamin D Receptor Expression in Vivo." Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 13, 2005, pp. 4724-8.
Healy KD, Vanhooke JL, Prahl JM, et al. Parathyroid hormone decreases renal vitamin D receptor expression in vivo. Proc Natl Acad Sci USA. 2005;102(13):4724-8.
Healy, K. D., Vanhooke, J. L., Prahl, J. M., & DeLuca, H. F. (2005). Parathyroid hormone decreases renal vitamin D receptor expression in vivo. Proceedings of the National Academy of Sciences of the United States of America, 102(13), pp. 4724-8.
Healy KD, et al. Parathyroid Hormone Decreases Renal Vitamin D Receptor Expression in Vivo. Proc Natl Acad Sci USA. 2005 Mar 29;102(13):4724-8. PubMed PMID: 15769857.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Parathyroid hormone decreases renal vitamin D receptor expression in vivo. AU - Healy,Kevin D, AU - Vanhooke,Janeen L, AU - Prahl,Jean M, AU - DeLuca,Hector F, Y1 - 2005/03/15/ PY - 2005/3/17/pubmed PY - 2005/6/10/medline PY - 2005/3/17/entrez SP - 4724 EP - 8 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc. Natl. Acad. Sci. U.S.A. VL - 102 IS - 13 N2 - The vitamin D receptor (VDR) is a nuclear transcription factor responsible for mediating the biological activities of 1,25-dihydroxyvitamin D(3) [1,25(OH)(2)D(3)]. Renal and parathyroid gland VDR content is an important factor in calcium homeostasis, vitamin D metabolism, and the treatment of secondary hyperparathyroidism and renal osteodystrophy. In these tissues, VDR expression is highly regulated by the calcium and vitamin D status. Although 1,25(OH)(2)D(3) up-regulates VDR expression, hypocalcemia and vitamin D deficiency result in drastically reduced expression of the receptor. The generation of 25-hydroxyvitamin D(3)-1alpha-hydroxylase-null mice, which are incapable of endogenously producing 1,25(OH)(2)D(3), has allowed us to investigate the influence of parathyroid hormone (PTH) on VDR expression independent of PTH-mediated increases in 1,25(OH)(2)D(3). Administration of human PTH (1-34) (110 microg/kg per day) for 48 h reduced renal VDR levels from 515 to 435 fmol/mg protein (15%, P < 0.03) in wild-type mice. In the 25-hydroxyvitamin D(3)-1alpha-hydroxylase-null mice, PTH administration strongly reduced renal VDR levels, from 555 to 394 fmol/mg protein (29%, P < 0.001). These results demonstrate that PTH is a potent down-regulator of VDR expression in vivo. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/15769857/Parathyroid_hormone_decreases_renal_vitamin_D_receptor_expression_in_vivo_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&amp;pmid=15769857 DB - PRIME DP - Unbound Medicine ER -