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Possible involvement of the interleukin-15 and interleukin-15 receptor system in a heightened state of lamina propria B cell activation and differentiation in patients with inflammatory bowel disease.
J Gastroenterol 2005; 40(2):128-36JG

Abstract

BACKGROUND

We investigated the possible roles of the interleukin (IL)-15 and IL-15 receptor (IL-15R) system in a heightened state of B-cell activation and differentiation in intestinal mucosa with inflammatory bowel disease (IBD).

METHODS

The expression of IL-15 and IL-15Ralpha mRNA and protein in inflamed colonic mucosal tissues with IBD, and in control tissues was examined by reverse transcriptase-polymerase chain reaction and immunohistological methods. The effects of recombinant (r)IL-15 on the expression of IL-15Ralpha on lamina propria B cells and the production of immunoglobulin G (IgG) were analyzed in vitro, using lamina propria mononuclear cells (LPMCs) isolated from control tissues.

RESULTS

The intensity of IL-15 and IL-15Ralpha mRNA was greater in the mucosal tissues of patients with IBD, especially in those of patients with ulcerative colitis (UC), than in control tissues. Compared to control tissues, mononuclear cells positive for IL-15Ralpha protein were observed in greater proportions in tissue sections from patients with IBD, especially in those from patients with UC, where IL-15Ralpha protein was localized to CD20-positive B cells to a significant degree. There were increases in the proportions of IL-15Ralpha-positive B cells and IgG-producing cells in rIL-15- or rCD40L-stimulated cultures of LPMCs, with stimulatory effects being greater in the presence of their combination.

CONCLUSIONS

These data suggest that the IL-15 and IL-15R system may play important roles in the activation and differentiation of lamina propria B cells in patients with IBD, especially in those with UC.

Authors+Show Affiliations

Department of Therapeutic Medicine, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15770395

Citation

Nishiwaki, Tsuyoshi, et al. "Possible Involvement of the Interleukin-15 and Interleukin-15 Receptor System in a Heightened State of Lamina Propria B Cell Activation and Differentiation in Patients With Inflammatory Bowel Disease." Journal of Gastroenterology, vol. 40, no. 2, 2005, pp. 128-36.
Nishiwaki T, Ina K, Goto H, et al. Possible involvement of the interleukin-15 and interleukin-15 receptor system in a heightened state of lamina propria B cell activation and differentiation in patients with inflammatory bowel disease. J Gastroenterol. 2005;40(2):128-36.
Nishiwaki, T., Ina, K., Goto, H., Watanabe, O., Tsuzuki, T., Furuta, R., ... Kusugami, K. (2005). Possible involvement of the interleukin-15 and interleukin-15 receptor system in a heightened state of lamina propria B cell activation and differentiation in patients with inflammatory bowel disease. Journal of Gastroenterology, 40(2), pp. 128-36.
Nishiwaki T, et al. Possible Involvement of the Interleukin-15 and Interleukin-15 Receptor System in a Heightened State of Lamina Propria B Cell Activation and Differentiation in Patients With Inflammatory Bowel Disease. J Gastroenterol. 2005;40(2):128-36. PubMed PMID: 15770395.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Possible involvement of the interleukin-15 and interleukin-15 receptor system in a heightened state of lamina propria B cell activation and differentiation in patients with inflammatory bowel disease. AU - Nishiwaki,Tsuyoshi, AU - Ina,Kenji, AU - Goto,Hidemi, AU - Watanabe,Osamu, AU - Tsuzuki,Tomoyuki, AU - Furuta,Ryuichi, AU - Ando,Takafumi, AU - Hibi,Kenji, AU - Kusugami,Kazuo, PY - 2004/05/19/received PY - 2004/09/16/accepted PY - 2005/3/17/pubmed PY - 2005/6/17/medline PY - 2005/3/17/entrez SP - 128 EP - 36 JF - Journal of gastroenterology JO - J. Gastroenterol. VL - 40 IS - 2 N2 - BACKGROUND: We investigated the possible roles of the interleukin (IL)-15 and IL-15 receptor (IL-15R) system in a heightened state of B-cell activation and differentiation in intestinal mucosa with inflammatory bowel disease (IBD). METHODS: The expression of IL-15 and IL-15Ralpha mRNA and protein in inflamed colonic mucosal tissues with IBD, and in control tissues was examined by reverse transcriptase-polymerase chain reaction and immunohistological methods. The effects of recombinant (r)IL-15 on the expression of IL-15Ralpha on lamina propria B cells and the production of immunoglobulin G (IgG) were analyzed in vitro, using lamina propria mononuclear cells (LPMCs) isolated from control tissues. RESULTS: The intensity of IL-15 and IL-15Ralpha mRNA was greater in the mucosal tissues of patients with IBD, especially in those of patients with ulcerative colitis (UC), than in control tissues. Compared to control tissues, mononuclear cells positive for IL-15Ralpha protein were observed in greater proportions in tissue sections from patients with IBD, especially in those from patients with UC, where IL-15Ralpha protein was localized to CD20-positive B cells to a significant degree. There were increases in the proportions of IL-15Ralpha-positive B cells and IgG-producing cells in rIL-15- or rCD40L-stimulated cultures of LPMCs, with stimulatory effects being greater in the presence of their combination. CONCLUSIONS: These data suggest that the IL-15 and IL-15R system may play important roles in the activation and differentiation of lamina propria B cells in patients with IBD, especially in those with UC. SN - 0944-1174 UR - https://www.unboundmedicine.com/medline/citation/15770395/Possible_involvement_of_the_interleukin_15_and_interleukin_15_receptor_system_in_a_heightened_state_of_lamina_propria_B_cell_activation_and_differentiation_in_patients_with_inflammatory_bowel_disease_ L2 - https://dx.doi.org/10.1007/s00535-004-1510-y DB - PRIME DP - Unbound Medicine ER -