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[Role of matrix metalloproteinase-2,9 and their inhibitors in premature rupture of membranes].
Zhonghua Fu Chan Ke Za Zhi. 2005 Jan; 40(1):29-33.ZF

Abstract

OBJECTIVE

To investigate the role of matrix metalloproteinase-2,9 (MMP-2, MMP-9) and tissue inhibitor of matrix metalloproteinases-2,1 (TIMP-2, TIMP-1) in human amniochorionic membrane.

METHODS

Amniochorionic membranes were collected from the following groups of women: (1) women with spontaneous premature rupture of membrane (PROM) before onset of labor (PROM group, n = 8), (2) Women with term labor after vaginal delivery (vaginal delivery group, n = 8), (3) Women undergoing elective cesarean section (C-section group, n = 8). Messenger ribonucleic acid expression for MMP-2, MMP-9, and their specific inhibitors TIMP-2 and TIMP-1 were studied with reverse transcriptase polymerase chain reaction.

RESULTS

(1) MMP-2 level in PROM group was 0.849 +/- 0.037; in vaginal delivery group 0.327 +/- 0.023; in C-section group 0.307 +/- 0.028. Expression in PROM group was highest, with significant difference compared with the other groups (P < 0.05). Its expression in vaginal delivery group and C-section group had no significant difference (P > 0.05). (2) MMP-9 level in PROM group was 0.026 +/- 0.004; in vaginal delivery group 0.008 +/- 0.001, with significant difference between the two groups (P < 0.05), while the expression was absent in C-section group. (3) TIMP-2 level in PROM group was 0.420 +/- 0.122; in vaginal delivery group 0.730 +/- 0.148; in C-section group 0.885 +/- 0.065. The expression in PROM group was significantly lower than in vaginal delivery group and C-section group (P < 0.05). And it was significantly lower in vaginal delivery group than in C-section group (P < 0.05). (4)TIMP-1 level in PROM group was 0.442 +/- 0.020; in vaginal delivery group 0.431 +/- 0.016; in C-section group 0.427 +/- 0.011. The expression in three groups had no significant difference (P > 0.05).

CONCLUSIONS

In PROM group, the expression of MMP-2, MMP-9 and their inhibitors TIMP-2, TIMP-1 is imbalanced, leading to increased extracellular matrix degradation, and weakening of the fetal membranes, and eventually premature rupture of the membranes.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15774089

Citation

Wang, Hong-lin, et al. "[Role of Matrix Metalloproteinase-2,9 and Their Inhibitors in Premature Rupture of Membranes]." Zhonghua Fu Chan Ke Za Zhi, vol. 40, no. 1, 2005, pp. 29-33.
Wang HL, Zhou C, Zhang YZ. [Role of matrix metalloproteinase-2,9 and their inhibitors in premature rupture of membranes]. Zhonghua Fu Chan Ke Za Zhi. 2005;40(1):29-33.
Wang, H. L., Zhou, C., & Zhang, Y. Z. (2005). [Role of matrix metalloproteinase-2,9 and their inhibitors in premature rupture of membranes]. Zhonghua Fu Chan Ke Za Zhi, 40(1), 29-33.
Wang HL, Zhou C, Zhang YZ. [Role of Matrix Metalloproteinase-2,9 and Their Inhibitors in Premature Rupture of Membranes]. Zhonghua Fu Chan Ke Za Zhi. 2005;40(1):29-33. PubMed PMID: 15774089.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Role of matrix metalloproteinase-2,9 and their inhibitors in premature rupture of membranes]. AU - Wang,Hong-lin, AU - Zhou,Chun, AU - Zhang,Yuan-zhen, PY - 2005/3/19/pubmed PY - 2006/11/7/medline PY - 2005/3/19/entrez SP - 29 EP - 33 JF - Zhonghua fu chan ke za zhi JO - Zhonghua Fu Chan Ke Za Zhi VL - 40 IS - 1 N2 - OBJECTIVE: To investigate the role of matrix metalloproteinase-2,9 (MMP-2, MMP-9) and tissue inhibitor of matrix metalloproteinases-2,1 (TIMP-2, TIMP-1) in human amniochorionic membrane. METHODS: Amniochorionic membranes were collected from the following groups of women: (1) women with spontaneous premature rupture of membrane (PROM) before onset of labor (PROM group, n = 8), (2) Women with term labor after vaginal delivery (vaginal delivery group, n = 8), (3) Women undergoing elective cesarean section (C-section group, n = 8). Messenger ribonucleic acid expression for MMP-2, MMP-9, and their specific inhibitors TIMP-2 and TIMP-1 were studied with reverse transcriptase polymerase chain reaction. RESULTS: (1) MMP-2 level in PROM group was 0.849 +/- 0.037; in vaginal delivery group 0.327 +/- 0.023; in C-section group 0.307 +/- 0.028. Expression in PROM group was highest, with significant difference compared with the other groups (P < 0.05). Its expression in vaginal delivery group and C-section group had no significant difference (P > 0.05). (2) MMP-9 level in PROM group was 0.026 +/- 0.004; in vaginal delivery group 0.008 +/- 0.001, with significant difference between the two groups (P < 0.05), while the expression was absent in C-section group. (3) TIMP-2 level in PROM group was 0.420 +/- 0.122; in vaginal delivery group 0.730 +/- 0.148; in C-section group 0.885 +/- 0.065. The expression in PROM group was significantly lower than in vaginal delivery group and C-section group (P < 0.05). And it was significantly lower in vaginal delivery group than in C-section group (P < 0.05). (4)TIMP-1 level in PROM group was 0.442 +/- 0.020; in vaginal delivery group 0.431 +/- 0.016; in C-section group 0.427 +/- 0.011. The expression in three groups had no significant difference (P > 0.05). CONCLUSIONS: In PROM group, the expression of MMP-2, MMP-9 and their inhibitors TIMP-2, TIMP-1 is imbalanced, leading to increased extracellular matrix degradation, and weakening of the fetal membranes, and eventually premature rupture of the membranes. SN - 0529-567X UR - https://www.unboundmedicine.com/medline/citation/15774089/[Role_of_matrix_metalloproteinase_29_and_their_inhibitors_in_premature_rupture_of_membranes]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=0529-567X&amp;year=2005&amp;vol=40&amp;issue=1&amp;fpage=29 DB - PRIME DP - Unbound Medicine ER -