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Chagas' disease: TCRBV9 over-representation and sequence oligoclonality in the fine specificity of T lymphocytes in target tissues of damage.
Acta Trop. 2005 Apr; 94(1):15-24.AT

Abstract

Using the same mouse strain and two Trypanosoma cruzi sub-populations (CA-I and RA) it is possible to induce pathology in different target tissues: skeletal muscle (CA-I) or sciatic nerve and spinal cord (RA). On the other hand, T cells are directly involved in tissue injury in a strain-dependent way, resembling the abnormalities of chronic Chagas' disease. In the present work, we examined the TCRBV repertoire and the CDR3 sequence polymorphism of T cells infiltrating spinal cord, sciatic nerve and skeletal muscle in chronically infected mice. The TCRBV9 segment was systematically over-represented in the target tissues for each T. cruzi strain: sciatic nerve and spinal cord in RA and skeletal muscle in CA-I-infected mice. The analysis of CDR3 sequence polymorphism in the same tissues showed a high proportion of identical TCRBV9 clones in RA-infected mice: 66.6% of the TCRBV9 clones found in sciatic nerve and spinal cord expressed one out of four major CDR3 rearrangements. Sequence identity was shared among clones from sciatic nerve and spinal cord, tissues that are also damaged by passive transfer of CD8 + TL. Those observations are consistent with an antigen driven T-cell expansion sequestered at the inflammation site and demonstrate -- for the first time -- the presence of an oligoclonal repertoire in the antigen recognition site of over-represented T cells in nervous system tissues in chronic Chagas' disease.

Authors+Show Affiliations

Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Argentina. valet@iib.unsam.edu.arNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15777704

Citation

Tekiel, Valeria, et al. "Chagas' Disease: TCRBV9 Over-representation and Sequence Oligoclonality in the Fine Specificity of T Lymphocytes in Target Tissues of Damage." Acta Tropica, vol. 94, no. 1, 2005, pp. 15-24.
Tekiel V, Oliveira GC, Correa-Oliveira R, et al. Chagas' disease: TCRBV9 over-representation and sequence oligoclonality in the fine specificity of T lymphocytes in target tissues of damage. Acta Trop. 2005;94(1):15-24.
Tekiel, V., Oliveira, G. C., Correa-Oliveira, R., Sánchez, D., & González-Cappa, S. M. (2005). Chagas' disease: TCRBV9 over-representation and sequence oligoclonality in the fine specificity of T lymphocytes in target tissues of damage. Acta Tropica, 94(1), 15-24.
Tekiel V, et al. Chagas' Disease: TCRBV9 Over-representation and Sequence Oligoclonality in the Fine Specificity of T Lymphocytes in Target Tissues of Damage. Acta Trop. 2005;94(1):15-24. PubMed PMID: 15777704.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chagas' disease: TCRBV9 over-representation and sequence oligoclonality in the fine specificity of T lymphocytes in target tissues of damage. AU - Tekiel,Valeria, AU - Oliveira,Guilherme C, AU - Correa-Oliveira,Rodrigo, AU - Sánchez,Daniel, AU - González-Cappa,Stella Maris, PY - 2004/11/03/received PY - 2005/01/26/revised PY - 2005/01/27/accepted PY - 2005/3/22/pubmed PY - 2005/5/25/medline PY - 2005/3/22/entrez SP - 15 EP - 24 JF - Acta tropica JO - Acta Trop VL - 94 IS - 1 N2 - Using the same mouse strain and two Trypanosoma cruzi sub-populations (CA-I and RA) it is possible to induce pathology in different target tissues: skeletal muscle (CA-I) or sciatic nerve and spinal cord (RA). On the other hand, T cells are directly involved in tissue injury in a strain-dependent way, resembling the abnormalities of chronic Chagas' disease. In the present work, we examined the TCRBV repertoire and the CDR3 sequence polymorphism of T cells infiltrating spinal cord, sciatic nerve and skeletal muscle in chronically infected mice. The TCRBV9 segment was systematically over-represented in the target tissues for each T. cruzi strain: sciatic nerve and spinal cord in RA and skeletal muscle in CA-I-infected mice. The analysis of CDR3 sequence polymorphism in the same tissues showed a high proportion of identical TCRBV9 clones in RA-infected mice: 66.6% of the TCRBV9 clones found in sciatic nerve and spinal cord expressed one out of four major CDR3 rearrangements. Sequence identity was shared among clones from sciatic nerve and spinal cord, tissues that are also damaged by passive transfer of CD8 + TL. Those observations are consistent with an antigen driven T-cell expansion sequestered at the inflammation site and demonstrate -- for the first time -- the presence of an oligoclonal repertoire in the antigen recognition site of over-represented T cells in nervous system tissues in chronic Chagas' disease. SN - 0001-706X UR - https://www.unboundmedicine.com/medline/citation/15777704/Chagas'_disease:_TCRBV9_over_representation_and_sequence_oligoclonality_in_the_fine_specificity_of_T_lymphocytes_in_target_tissues_of_damage_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0001-706X(05)00030-6 DB - PRIME DP - Unbound Medicine ER -