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Isoflurane and sevoflurane affect cell survival and BCL-2/BAX ratio differently.
Brain Res. 2005 Mar 10; 1037(1-2):139-47.BR

Abstract

Depletion of calcium from the neuronal endoplasmic reticulum (ER) induces apoptosis. Isoflurane depletes calcium from sarcoplasmic reticulum (SR) of muscle, an analogue of ER in neurons, while sevoflurane maintains or increases SR calcium. We hypothesized that isoflurane, but not sevoflurane, induces apoptosis by depleting the ER calcium. Rat PC12 pheochromocytoma cells and primary cortical neurons were treated with equipotent doses of isoflurane and sevoflurane. Isoflurane, but not sevoflurane, at equipotent doses induced cell damage determined by both LDH release and MTT reduction assays, dose and time dependently, in both types of cells. Isoflurane at 2.4% for 24 h induced cytotoxicity in both cell types, which was characterized by nuclear condensation and fragmentation and activation of caspases 3 and 9. Isoflurane cytotoxicity was suppressed by dantrolene, a ryanodine receptor antagonist that inhibits abnormal calcium release from the ER. Isoflurane decreased the Bcl-2/Bax ratio by as much as 36% (P < 0.05). However, sevoflurane did not cause neuronal damage by apoptosis nor did it decrease the Bcl-2/Bax ratio. These results suggest that isoflurane and sevoflurane differentially affect the Bcl-2/Bax ratio and cell survival. At equipotent concentrations, isoflurane, but not sevoflurane, induces cytotoxicity in both PC12 cells and primary cortical neurons and decreases the Bcl-2/Bax ratio.

Authors+Show Affiliations

Department of Anesthesia, 305 John Morgan Building, 3620 Hamilton Walk, University of Pennsylvania Health System, Philadelphia, PA 19104-4283, USA. weih@uphs.upenn.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15777762

Citation

Wei, Huafeng, et al. "Isoflurane and Sevoflurane Affect Cell Survival and BCL-2/BAX Ratio Differently." Brain Research, vol. 1037, no. 1-2, 2005, pp. 139-47.
Wei H, Kang B, Wei W, et al. Isoflurane and sevoflurane affect cell survival and BCL-2/BAX ratio differently. Brain Res. 2005;1037(1-2):139-47.
Wei, H., Kang, B., Wei, W., Liang, G., Meng, Q. C., Li, Y., & Eckenhoff, R. G. (2005). Isoflurane and sevoflurane affect cell survival and BCL-2/BAX ratio differently. Brain Research, 1037(1-2), 139-47.
Wei H, et al. Isoflurane and Sevoflurane Affect Cell Survival and BCL-2/BAX Ratio Differently. Brain Res. 2005 Mar 10;1037(1-2):139-47. PubMed PMID: 15777762.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isoflurane and sevoflurane affect cell survival and BCL-2/BAX ratio differently. AU - Wei,Huafeng, AU - Kang,Baobin, AU - Wei,Wenlin, AU - Liang,Ge, AU - Meng,Qing Cheng, AU - Li,Yujuan, AU - Eckenhoff,Roderic G, PY - 2004/08/10/received PY - 2004/12/17/revised PY - 2005/01/01/accepted PY - 2005/3/22/pubmed PY - 2005/6/17/medline PY - 2005/3/22/entrez SP - 139 EP - 47 JF - Brain research JO - Brain Res. VL - 1037 IS - 1-2 N2 - Depletion of calcium from the neuronal endoplasmic reticulum (ER) induces apoptosis. Isoflurane depletes calcium from sarcoplasmic reticulum (SR) of muscle, an analogue of ER in neurons, while sevoflurane maintains or increases SR calcium. We hypothesized that isoflurane, but not sevoflurane, induces apoptosis by depleting the ER calcium. Rat PC12 pheochromocytoma cells and primary cortical neurons were treated with equipotent doses of isoflurane and sevoflurane. Isoflurane, but not sevoflurane, at equipotent doses induced cell damage determined by both LDH release and MTT reduction assays, dose and time dependently, in both types of cells. Isoflurane at 2.4% for 24 h induced cytotoxicity in both cell types, which was characterized by nuclear condensation and fragmentation and activation of caspases 3 and 9. Isoflurane cytotoxicity was suppressed by dantrolene, a ryanodine receptor antagonist that inhibits abnormal calcium release from the ER. Isoflurane decreased the Bcl-2/Bax ratio by as much as 36% (P < 0.05). However, sevoflurane did not cause neuronal damage by apoptosis nor did it decrease the Bcl-2/Bax ratio. These results suggest that isoflurane and sevoflurane differentially affect the Bcl-2/Bax ratio and cell survival. At equipotent concentrations, isoflurane, but not sevoflurane, induces cytotoxicity in both PC12 cells and primary cortical neurons and decreases the Bcl-2/Bax ratio. SN - 0006-8993 UR - https://www.unboundmedicine.com/medline/citation/15777762/Isoflurane_and_sevoflurane_affect_cell_survival_and_BCL_2/BAX_ratio_differently_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(05)00052-1 DB - PRIME DP - Unbound Medicine ER -