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Circulating human antibodies against dengue NS1 protein: potential of recombinant D2V-NS1 proteins in diagnostic tests.
J Clin Virol. 2005 Apr; 32(4):305-12.JC

Abstract

The dengue virus (DV) causes one of the most important arthropod-borne human viral diseases throughout the tropical and subtropical countries. However, the morbidity and mortality of DV infections could be reduced with an early hospitalization care and a rapid risk identification of developing the dengue haemorrhagic fever (DHF). The nonstructural glycoprotein 1 (NS1) has been pointed as a reagent for immune-assay diagnostic test optimization. To evaluate this potential, recombinant DV2-NS1 proteins (rNS1) were produced from Escherichia coli (NS1EC) and insect cells (NS1IC) expression. The tests were performed by analysis of a human serum panel reacted against different rNS1 forms. The results demonstrated high correspondence between the DV positive sera and the assay results using native or refolded forms of either NS1IC or NS1EC. Also, the IgG and IgM anti-rNS1 level profiles showed distinct distribution, depending on protein form and disease status. However, the IgM anti-rNS1 reactions did not show sensibility to detect the DV in primary infections. The data obtained from the paired serum samples reactivity comparison suggested a heterogeneous human immune response and absence of correspondence between the IgG and IgM profile levels. Moreover, a patient with negative reference test could be detected by specific IgG anti-rNS1 assays presented here. Therefore, these results sustain the usefulness of dengue nonstructural proteins, in particular the NS1, in diagnostic tests as a complementary reagent.

Authors+Show Affiliations

Laboratório de Tecnologia Recombinante, Bio-Manguinhos, FIOCRUZ, Av. Brasil, 4365 Manguinhos, Rio de Janeiro, 21045-900 RJ, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15780810

Citation

Lemes, E M B., et al. "Circulating Human Antibodies Against Dengue NS1 Protein: Potential of Recombinant D2V-NS1 Proteins in Diagnostic Tests." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 32, no. 4, 2005, pp. 305-12.
Lemes EM, Miagostovicsh MP, Alves AM, et al. Circulating human antibodies against dengue NS1 protein: potential of recombinant D2V-NS1 proteins in diagnostic tests. J Clin Virol. 2005;32(4):305-12.
Lemes, E. M., Miagostovicsh, M. P., Alves, A. M., Costa, S. M., Fillipis, A. M., Armoa, G. R., & Araujo, M. A. (2005). Circulating human antibodies against dengue NS1 protein: potential of recombinant D2V-NS1 proteins in diagnostic tests. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 32(4), 305-12.
Lemes EM, et al. Circulating Human Antibodies Against Dengue NS1 Protein: Potential of Recombinant D2V-NS1 Proteins in Diagnostic Tests. J Clin Virol. 2005;32(4):305-12. PubMed PMID: 15780810.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Circulating human antibodies against dengue NS1 protein: potential of recombinant D2V-NS1 proteins in diagnostic tests. AU - Lemes,E M B, AU - Miagostovicsh,M P, AU - Alves,A M B, AU - Costa,S M, AU - Fillipis,A M B, AU - Armoa,G R G, AU - Araujo,M A V, PY - 2004/07/28/received PY - 2004/08/30/accepted PY - 2005/3/23/pubmed PY - 2005/9/21/medline PY - 2005/3/23/entrez SP - 305 EP - 12 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J Clin Virol VL - 32 IS - 4 N2 - The dengue virus (DV) causes one of the most important arthropod-borne human viral diseases throughout the tropical and subtropical countries. However, the morbidity and mortality of DV infections could be reduced with an early hospitalization care and a rapid risk identification of developing the dengue haemorrhagic fever (DHF). The nonstructural glycoprotein 1 (NS1) has been pointed as a reagent for immune-assay diagnostic test optimization. To evaluate this potential, recombinant DV2-NS1 proteins (rNS1) were produced from Escherichia coli (NS1EC) and insect cells (NS1IC) expression. The tests were performed by analysis of a human serum panel reacted against different rNS1 forms. The results demonstrated high correspondence between the DV positive sera and the assay results using native or refolded forms of either NS1IC or NS1EC. Also, the IgG and IgM anti-rNS1 level profiles showed distinct distribution, depending on protein form and disease status. However, the IgM anti-rNS1 reactions did not show sensibility to detect the DV in primary infections. The data obtained from the paired serum samples reactivity comparison suggested a heterogeneous human immune response and absence of correspondence between the IgG and IgM profile levels. Moreover, a patient with negative reference test could be detected by specific IgG anti-rNS1 assays presented here. Therefore, these results sustain the usefulness of dengue nonstructural proteins, in particular the NS1, in diagnostic tests as a complementary reagent. SN - 1386-6532 UR - https://www.unboundmedicine.com/medline/citation/15780810/Circulating_human_antibodies_against_dengue_NS1_protein:_potential_of_recombinant_D2V_NS1_proteins_in_diagnostic_tests_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(04)00247-1 DB - PRIME DP - Unbound Medicine ER -