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Pho d 2, a major allergen from date palm pollen, is a profilin: cloning, sequencing, and immunoglobulin E cross-reactivity with other profilins.
Clin Exp Allergy. 2005 Mar; 35(3):374-81.CE

Abstract

BACKGROUND

Up to now, some date palm pollen (DPP) allergens have been described but very few data are available about their molecular nature. The aim of this study was to identify and characterize Pho d 2, a major allergen from this pollen.

METHODS

Sera from 25 patients allergic to DPP were analysed by immunoblotting. Purification of DPP profilin was performed by poly-l-proline affinity chromatography. Profilin-encoding cDNA from DPP was cloned by using a RT-PCR strategy and recombinant allergen was expressed as a non-fusion protein in Escherichia coli. Natural and recombinant Pho d 2 were investigated by means of enzyme allergosorbent test to compare the immunologic properties of both allergens and to analyse cross-reactivity with other profilins.

RESULTS

A 14.4 kDa protein was identified as a major allergen in DPP extract. Purification, cloning, heterologous expression, and inhibition experiments identified it as profilin (Pho d 2). Pho d 2 comprises 131 amino acids and has high sequence identity with other allergenic food and pollen profilins. The prevalence of specific IgE antibody reactivity to natural Pho d 2 by ELISA was 56% and 64% by skin prick test (SPT). Pho d 2 is an important allergen as it is responsible for more than 70% of the IgE reactivity to the pollen extract. IgE directed against Pho d 2 showed a strong cross-reactivity with other profilins such as those from olive tree and grass pollens.

CONCLUSION

Pho d 2, a 14.4 kDa protein identified as profilin, is a major and relevant allergen in DPP, as confirmed by SPT and thereby may elicit clinical symptoms in sensitized patients.

Authors+Show Affiliations

Department of Research and Development, Bial-Arístegui, E-48008 Bilbao, Spain. juan.asturias@bial.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15784118

Citation

Asturias, J A., et al. "Pho D 2, a Major Allergen From Date Palm Pollen, Is a Profilin: Cloning, Sequencing, and Immunoglobulin E Cross-reactivity With Other Profilins." Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, vol. 35, no. 3, 2005, pp. 374-81.
Asturias JA, Ibarrola I, Fernández J, et al. Pho d 2, a major allergen from date palm pollen, is a profilin: cloning, sequencing, and immunoglobulin E cross-reactivity with other profilins. Clin Exp Allergy. 2005;35(3):374-81.
Asturias, J. A., Ibarrola, I., Fernández, J., Arilla, M. C., González-Rioja, R., & Martínez, A. (2005). Pho d 2, a major allergen from date palm pollen, is a profilin: cloning, sequencing, and immunoglobulin E cross-reactivity with other profilins. Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, 35(3), 374-81.
Asturias JA, et al. Pho D 2, a Major Allergen From Date Palm Pollen, Is a Profilin: Cloning, Sequencing, and Immunoglobulin E Cross-reactivity With Other Profilins. Clin Exp Allergy. 2005;35(3):374-81. PubMed PMID: 15784118.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pho d 2, a major allergen from date palm pollen, is a profilin: cloning, sequencing, and immunoglobulin E cross-reactivity with other profilins. AU - Asturias,J A, AU - Ibarrola,I, AU - Fernández,J, AU - Arilla,M C, AU - González-Rioja,R, AU - Martínez,A, PY - 2005/3/24/pubmed PY - 2005/9/1/medline PY - 2005/3/24/entrez SP - 374 EP - 81 JF - Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology JO - Clin Exp Allergy VL - 35 IS - 3 N2 - BACKGROUND: Up to now, some date palm pollen (DPP) allergens have been described but very few data are available about their molecular nature. The aim of this study was to identify and characterize Pho d 2, a major allergen from this pollen. METHODS: Sera from 25 patients allergic to DPP were analysed by immunoblotting. Purification of DPP profilin was performed by poly-l-proline affinity chromatography. Profilin-encoding cDNA from DPP was cloned by using a RT-PCR strategy and recombinant allergen was expressed as a non-fusion protein in Escherichia coli. Natural and recombinant Pho d 2 were investigated by means of enzyme allergosorbent test to compare the immunologic properties of both allergens and to analyse cross-reactivity with other profilins. RESULTS: A 14.4 kDa protein was identified as a major allergen in DPP extract. Purification, cloning, heterologous expression, and inhibition experiments identified it as profilin (Pho d 2). Pho d 2 comprises 131 amino acids and has high sequence identity with other allergenic food and pollen profilins. The prevalence of specific IgE antibody reactivity to natural Pho d 2 by ELISA was 56% and 64% by skin prick test (SPT). Pho d 2 is an important allergen as it is responsible for more than 70% of the IgE reactivity to the pollen extract. IgE directed against Pho d 2 showed a strong cross-reactivity with other profilins such as those from olive tree and grass pollens. CONCLUSION: Pho d 2, a 14.4 kDa protein identified as profilin, is a major and relevant allergen in DPP, as confirmed by SPT and thereby may elicit clinical symptoms in sensitized patients. SN - 0954-7894 UR - https://www.unboundmedicine.com/medline/citation/15784118/Pho_d_2_a_major_allergen_from_date_palm_pollen_is_a_profilin:_cloning_sequencing_and_immunoglobulin_E_cross_reactivity_with_other_profilins_ L2 - https://doi.org/10.1111/j.1365-2222.2005.02179.x DB - PRIME DP - Unbound Medicine ER -