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Apoplastic oxidation of L-asparagine is involved in the control of the green algal endophyte Acrochaete operculata Correa & Nielsen by the red seaweed Chondrus crispus Stackhouse.
J Exp Bot. 2005 May; 56(415):1317-26.JE

Abstract

Gametophytes of the marine alga Chondrus crispus are more resistant than tetrasporophytes to infection by the filamentous endophytic alga Acrochaete operculata. It has been shown recently that carrageenan oligosaccharides from the resistant gametophytic generation of C. crispus stimulate the secretion of L-asparagine (L-Asn) by the endophyte and that the host generates hydrogen peroxide and 2-oxo-succinamic acid after contact with this amino acid. Here the response of C. crispus to L-Asn and its effect on the pathogen is investigated. Chondrus crispus released hydrogen peroxide, ammonium ions, and a carbonyl compound into the medium when exposed to L-Asn. This response was correlated with an increase in oxygen consumption. Inhibitor studies indicated the involvement of a flavoenzyme in the reaction, which was sensitive to high concentrations of the reaction product, ammonium, and to chlorpromazine, quinacrine, and cyanide, inhibitors of L-amino acid oxidase. Cell wall macerate of C. crispus also responded to L-Asn, while protoplasts were inactive. Uptake of L-Asn into the cell was not necessary for the response, suggesting that the involved L-amino acid oxidase is apoplastic. Acrochaete operculata was more sensitive to hydrogen peroxide than C. crispus and settlement of A. operculata zoospores on C. crispus was reduced by 86% in the presence of L-Asn. This reduced settlement could be prevented with catalase. Chondrus crispus thus features an apoplastic amino acid oxidase, which is involved in the control of its endophytic pathogen. The modulation of the amino acid secretion in A. operculata by carrageenan oligosaccharides is therefore a key issue in the etiology of the association.

Authors+Show Affiliations

UMR 7139 CNRS, Université Pierre et Marie Curie and Laboratoires Goëmar, Station Biologique, Place Georges Teissier, F-29680 Roscoff, France. weinberg@sb-roscoff.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15784529

Citation

Weinberger, Florian, et al. "Apoplastic Oxidation of L-asparagine Is Involved in the Control of the Green Algal Endophyte Acrochaete Operculata Correa & Nielsen By the Red Seaweed Chondrus Crispus Stackhouse." Journal of Experimental Botany, vol. 56, no. 415, 2005, pp. 1317-26.
Weinberger F, Pohnert G, Berndt ML, et al. Apoplastic oxidation of L-asparagine is involved in the control of the green algal endophyte Acrochaete operculata Correa & Nielsen by the red seaweed Chondrus crispus Stackhouse. J Exp Bot. 2005;56(415):1317-26.
Weinberger, F., Pohnert, G., Berndt, M. L., Bouarab, K., Kloareg, B., & Potin, P. (2005). Apoplastic oxidation of L-asparagine is involved in the control of the green algal endophyte Acrochaete operculata Correa & Nielsen by the red seaweed Chondrus crispus Stackhouse. Journal of Experimental Botany, 56(415), 1317-26.
Weinberger F, et al. Apoplastic Oxidation of L-asparagine Is Involved in the Control of the Green Algal Endophyte Acrochaete Operculata Correa & Nielsen By the Red Seaweed Chondrus Crispus Stackhouse. J Exp Bot. 2005;56(415):1317-26. PubMed PMID: 15784529.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Apoplastic oxidation of L-asparagine is involved in the control of the green algal endophyte Acrochaete operculata Correa & Nielsen by the red seaweed Chondrus crispus Stackhouse. AU - Weinberger,Florian, AU - Pohnert,Georg, AU - Berndt,Mary-Lynn, AU - Bouarab,Kamal, AU - Kloareg,Bernard, AU - Potin,Philippe, Y1 - 2005/03/21/ PY - 2005/3/24/pubmed PY - 2005/8/12/medline PY - 2005/3/24/entrez SP - 1317 EP - 26 JF - Journal of experimental botany JO - J Exp Bot VL - 56 IS - 415 N2 - Gametophytes of the marine alga Chondrus crispus are more resistant than tetrasporophytes to infection by the filamentous endophytic alga Acrochaete operculata. It has been shown recently that carrageenan oligosaccharides from the resistant gametophytic generation of C. crispus stimulate the secretion of L-asparagine (L-Asn) by the endophyte and that the host generates hydrogen peroxide and 2-oxo-succinamic acid after contact with this amino acid. Here the response of C. crispus to L-Asn and its effect on the pathogen is investigated. Chondrus crispus released hydrogen peroxide, ammonium ions, and a carbonyl compound into the medium when exposed to L-Asn. This response was correlated with an increase in oxygen consumption. Inhibitor studies indicated the involvement of a flavoenzyme in the reaction, which was sensitive to high concentrations of the reaction product, ammonium, and to chlorpromazine, quinacrine, and cyanide, inhibitors of L-amino acid oxidase. Cell wall macerate of C. crispus also responded to L-Asn, while protoplasts were inactive. Uptake of L-Asn into the cell was not necessary for the response, suggesting that the involved L-amino acid oxidase is apoplastic. Acrochaete operculata was more sensitive to hydrogen peroxide than C. crispus and settlement of A. operculata zoospores on C. crispus was reduced by 86% in the presence of L-Asn. This reduced settlement could be prevented with catalase. Chondrus crispus thus features an apoplastic amino acid oxidase, which is involved in the control of its endophytic pathogen. The modulation of the amino acid secretion in A. operculata by carrageenan oligosaccharides is therefore a key issue in the etiology of the association. SN - 0022-0957 UR - https://www.unboundmedicine.com/medline/citation/15784529/Apoplastic_oxidation_of_L_asparagine_is_involved_in_the_control_of_the_green_algal_endophyte_Acrochaete_operculata_Correa_&_Nielsen_by_the_red_seaweed_Chondrus_crispus_Stackhouse_ L2 - https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/eri132 DB - PRIME DP - Unbound Medicine ER -