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Conditional and inducible transgene expression in mice through the combinatorial use of Cre-mediated recombination and tetracycline induction.
Nucleic Acids Res. 2005 Mar 22; 33(5):e51.NA

Abstract

Here we describe a triple transgenic mouse system, which combines the tissue specificity of any Cre-transgenic line with the inducibility of the reverse tetracycline transactivator (rtTA)/tetracycline-responsive element (tet-O)-driven transgenes. To ensure reliable rtTA expression in a broad range of cell types, we have targeted the rtTA transgene into the ROSA26 locus. The rtTA expression, however, is conditional to a Cre recombinase-mediated excision of a STOP region from the ROSA26 locus. We demonstrate the utility of this technology through the inducible expression of the vascular endothelial growth factor (VEGF-A) during embryonic development and postnatally in adult mice. Our results of adult induction recapitulate several different hepatic and immune cell pathological phenotypes associated with increased systemic VEGF-A protein levels. This system will be useful for studying genes in which temporal control of expression is necessary for the discovery of the full spectrum of functions. The presented approach abrogates the need to generate tissue-specific rtTA transgenes for tissues where well-characterized Cre lines already exist.

Authors+Show Affiliations

Samuel Lunenfeld Research Institute, Mount Sinai Hospital 600 University Avenue, Toronto, Ontario, Canada M5G 1X5.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15784609

Citation

Belteki, Gusztav, et al. "Conditional and Inducible Transgene Expression in Mice Through the Combinatorial Use of Cre-mediated Recombination and Tetracycline Induction." Nucleic Acids Research, vol. 33, no. 5, 2005, pp. e51.
Belteki G, Haigh J, Kabacs N, et al. Conditional and inducible transgene expression in mice through the combinatorial use of Cre-mediated recombination and tetracycline induction. Nucleic Acids Res. 2005;33(5):e51.
Belteki, G., Haigh, J., Kabacs, N., Haigh, K., Sison, K., Costantini, F., Whitsett, J., Quaggin, S. E., & Nagy, A. (2005). Conditional and inducible transgene expression in mice through the combinatorial use of Cre-mediated recombination and tetracycline induction. Nucleic Acids Research, 33(5), e51.
Belteki G, et al. Conditional and Inducible Transgene Expression in Mice Through the Combinatorial Use of Cre-mediated Recombination and Tetracycline Induction. Nucleic Acids Res. 2005 Mar 22;33(5):e51. PubMed PMID: 15784609.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Conditional and inducible transgene expression in mice through the combinatorial use of Cre-mediated recombination and tetracycline induction. AU - Belteki,Gusztav, AU - Haigh,Jody, AU - Kabacs,Nikolett, AU - Haigh,Katharina, AU - Sison,Karen, AU - Costantini,Frank, AU - Whitsett,Jeff, AU - Quaggin,Susan E, AU - Nagy,Andras, Y1 - 2005/03/22/ PY - 2005/3/24/pubmed PY - 2005/4/16/medline PY - 2005/3/24/entrez SP - e51 EP - e51 JF - Nucleic acids research JO - Nucleic Acids Res VL - 33 IS - 5 N2 - Here we describe a triple transgenic mouse system, which combines the tissue specificity of any Cre-transgenic line with the inducibility of the reverse tetracycline transactivator (rtTA)/tetracycline-responsive element (tet-O)-driven transgenes. To ensure reliable rtTA expression in a broad range of cell types, we have targeted the rtTA transgene into the ROSA26 locus. The rtTA expression, however, is conditional to a Cre recombinase-mediated excision of a STOP region from the ROSA26 locus. We demonstrate the utility of this technology through the inducible expression of the vascular endothelial growth factor (VEGF-A) during embryonic development and postnatally in adult mice. Our results of adult induction recapitulate several different hepatic and immune cell pathological phenotypes associated with increased systemic VEGF-A protein levels. This system will be useful for studying genes in which temporal control of expression is necessary for the discovery of the full spectrum of functions. The presented approach abrogates the need to generate tissue-specific rtTA transgenes for tissues where well-characterized Cre lines already exist. SN - 1362-4962 UR - https://www.unboundmedicine.com/medline/citation/15784609/Conditional_and_inducible_transgene_expression_in_mice_through_the_combinatorial_use_of_Cre_mediated_recombination_and_tetracycline_induction_ L2 - https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gni051 DB - PRIME DP - Unbound Medicine ER -